Dicer requiring for astrocyte quiescence in developing brains

doi:10.16098/j.issn.0529-1356.2022.04.001

Abstract

Abstract:

Objective To explore whether microRNAs(miRNA) in astrocytes participate in regulating the phenotypic switch in the developing central nervous system(CNS). Methods The hGFAP-CreERT;Dicer fl/fl;mT/mG and Dicer fl/fl;mT/mG mice were generated and induced by tamoxifen(TMF). They were divided into 7 days, 10 days, 14 days Dicer conditional knock out (Dicer CKO) group and control group according to their age and the expression of hGFAP-CreERT. These 24 mice were divided into six groups and each group contained four mice. Then the change of astrocytes in the developing CNS was observed by immunofluorescenct staining. Results Both of the densities of astrocytes and microglial cells increased in the Dicer CKO mice brains as compared to littermate controls. Conclusion Dicer and miRNA of astrocytes are important for astrocytes quiescence in developing brains.

Key words: White matte, Astrocyte, microRNA, Microglia, Glial fibrillary acidic protein, Immunofluorescence, Mouse

CLC Number:

Q189

LIU Kun CHEN Jing-fei WANG Shou-yu LI Tao GAO Xing YU Bin MEI Feng XIAO Lan. Dicer requiring for astrocyte quiescence in developing brains[J]. Acta Anatomica Sinica, 2022, 53(4): 407-411.

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