Analysis of Human Brain Bank samples from Hebei Medical University

doi:10.16098/j.issn.0529-1356.2024.04.009

Abstract

Abstract:

Objective To understand the current situation of human brain donation in Hebei Province by analyzing the basic information of Human Brain Bank samples of Hebei Medical University in order to provide basic data support for subsequent scientific research. Methods The samples collected from the Human Brain Bank of Hebei Medical University were analyzed (from December 2019 to February 2024), including gender, age, cause of death, as well as quality control data such as postmortem delay time, pH value of cerebrospinal fluid and and RNA integrity number and result of neuropathological diagnosis. Results Until February 2024, 30 human brain samples were collected and stored in the Human Brain Bank of Hebei Medical University, with a male to female ratio of 9∶1. Donors over 80 years old accounted for 53%. Cardiovascular and cerebrovascular diseases (36.67%) and nervous system diseases (23.33%) accounted for a high proportion of the death causes. The location of brain tissue donors in Shijiazhuang accounted for 90% donations, and the others were from outside the city. The postmortem delay time was relatively short, 90% within 12 hours and 10 % more than 12 hours. 69.23% of the brain samples had RNA integrity values greater than 6. Cerebrospinal fluid pH values ranged from 5.8 to 7.5, with an average value of 6.60 ± 0.45. Brain weights ranged from 906-1496g, with an average value of（1210.78 ± 197.84）g. Three apolipoprotein E (APOE) alleles were detected including five genotypes (ε2/ε3, ε2/ε4, ε3/ε3, ε3/ε4, ε4/ε4). Eleven staining methods related to neuropathological diagnosis had been established and used. A total of 12 cases were diagnosed as neurodegenerative diseases (including Alzheimer’s disease, Parkinson’s disease, multiple system atrophy, corticobasal degeneration and progressive supranuclear palsy，etc.), accounting for 40% donated brains. The comorbidity rate of samples over 80 years old was 100%. Conclusion The summary and analyses of the data of brain donors in the Human Brain Bank of Hebei Medical University can reflect the current situation of the construction and operation of the brain bank in Hebei Province, and it can also be more targeted to understand and identify potential donors. Our information can provide reference for the construction of brain bank and provides more reliable materials and data support for scientific research.

Key words: Human Brain Bank, Human brain sample, Basic information, Quality control information, Neuropathological diagnosis, Neurodegenerative disease, Comorbidity

CLC Number:

R741

DU Juan MI Shi-xiong JIN Yu-chuan YANG Qian MA Min ZHAO Xue-ru LIU Feng-cang ZHAO Chang-yi ZHANG Zhan-chi FAN Ping CUI Hui-xian. Analysis of Human Brain Bank samples from Hebei Medical University[J]. Acta Anatomica Sinica, 2024, 55(4): 437-444.

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