Expression of dysbindin-1 during spermatogenesis

doi:10.16098/j.issn.0529-1356.2015.04.018

Abstract

Abstract:

Objective To test dysbindin-1 expression during spermatogenesis and dysbindin-1 effect on histology of acrosome in mice. Methods Western blotting analysis was applied to detect the expression of dysbindin-1 in testis on day 7,14,21,28 and 35 postpartum and three-month old mice(n=3). The caudal spermatozoa was collected from sdy mice (no dysbindin-1expression), HE staining was performed for sperm morphology, fluorescein isothiocyanate conjugate-pisum sativum agglutinin (FITC-PSA) staining and immunofluorescence staining with antibody against sperm protein 56(sp56) for acrosome on sperm smear. Results Dysbindin-1A, not -1C, was expressed in postnatal testis development and spermatozoa. Mature sperm from sdy caudal epididymis had normal formation. Conclusion Dysbindin-1A, expressed in testis tissue and sperm during spermatogenesis, is not required for maintaining the morphology of sperm.

Key words: Dysbindin-1, Testis, Sperm, Acrosome, Western blotting, sdy mouse, Mouse

YAN Hui LIU Hui-xia WEI Zong-bo GUO Rui ZHANG Tao FENG Ya-qin*. Expression of dysbindin-1 during spermatogenesis[J]. AAS, 2015, 46(4): 548-552.

References

［1］Wei AH, Li W. Hermansky-Pudlak syndrome: pigmentary and non-pigmentary defects and their pathogenesis ［J］. Pigment Cell Melanoma Res, 2013, 26(2): 176-192.
［2］Li W, Feng YQ, Hao CJ, et al. The BLOC interactomes form a network in endosomal transport ［J］. J Genet Genomics, 2007, 34(8): 669-682.
［3］Li W, Zhang Q, Oiso N, et al. Hermansky-Pudlak syndrome type 7 (HPS-7) results from mutant dysbindin, a member of the biogenesis of lysosome-related organelles complex 1 (BLOC-1) ［J］. Nat Genet, 2003, 35(1): 84-89.
［4］Benson MA, Newey SE, Martin-Rendon E, et al. Dysbindin, a novel coiled-coil-containing protein that interacts with the dystrobrevins in muscle and brain ［J］. J Biol Chem, 2001, 2769(26): 24232-24241.
［5］Chen XW, Feng YQ, Hao CJ, et al. DTNBP1, a schizophrenia susceptiblility gene, affects kinetics of transmitter release ［J］. J Cell Biol, 2008, 18(5): 791-801.
［6］Wang H, Yuan YF, Zhang Z, et al. Dysbindin1C is required for the survival of hilar mossy cells and the maturation of adult newborn neurons in dentate gyrus ［J］. J Biol Chem, 2014, 289(42): 29060-29072.
［7］Berruti G, Ripolone M, Ceriani M. USP8, a regulator of endosomal sorting, is involved in mouse acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules ［J］. Biol Reprod, 2010, 82(5): 930-939.
［8］Nian H, Fan CH, Liao SY, et al. RNF151, a testis-specific RING finger protein, interacts with dysbindin ［J］. Arch Biochem Biophys, 2007, 465(1): 157-163.
［9］Yan S, Zhou CQ, Zhang W, et al. Beta-catenin/TCF pathway upregulates STAT3 expression in human esophageal squamous cell carcinoma ［J］. Cancer Letters, 2008, 271(1): 85-97.
［10］Zhao LM, Reim K, David JM. Complexin-I-deficient sperm are subfertile due to a defect in zona pellucida penetration ［J］. Reproduction, 2008, 136(3): 323-334.
［11］Ana LD, Marvin LM, Helio CG. Spermatogonial morphology and kinetics during testis development in mice: a high-resolution light microscopyapproach ［J］. Reproduction, 2011, 142(1):145-155.
［12］Zhou M,Wang ShP. Histological and histochemical study on the development and differentiation of mouse testes ［J］. Acta Anatomica Sinica, 1987, 18(4):434-438. (in Chinese)
周明,王士平. 小白鼠睾丸发育分化的组织学及组织化学观察［J］. 解剖学报，1987,18(4): 434-438.
［13］Berruti G, Paiardi C. Acrosome biogenesis: revisiting old questions to yield new insights ［J］. Spermatogenesis, 2011, 1(2): 95-98.
［14］Setty SR, Tenza D, Truschel ST, et al. BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles ［J］. Mol Biol Cell, 2007, 18(3):768-780.
［15］Ji YY, Yang F, Papaleo F, et al. Role of dysbindin in dopamine receptor trafficking and cortical GABA function ［J］. Proc Natl Acad Sci USA, 2009, 106(46):19593-19598.
［16］Cai Q, Lu L, Tian JH, et al. Snapin-regulated late endosomal transport is critical for efficient autophagy-lysosomal function in neurons ［J］. Neuron, 2010, 68(1):73-86.

[1]	HONG Xiao-min LI San-qiang CUI Qin-yi ZHENG Run-yue YANG Meng-li LUO Ren-li LI Qian-hui. Nuclear factor-κB signaling pathway and gender differences in alcoholic liver fibrosis [J]. Acta Anatomica Sinica, 2024, 55(1): 55-61.
[2]	WEI Xi-xi LI Chao-hong ZHAO Chen-lu TANG Jia-ping LIU Yu-zhen. Characterization of group Ⅰ metabotropic glutamate receptors in rat superior cervical ganglion and their changes following chronic intermittent hypoxia [J]. Acta Anatomica Sinica, 2024, 55(1): 3-9.
[3]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[4]	MA Ting-ting CHEN Jian-hong LIU Ai-cui LI Hai-ning. Role of inhibiting lncRNA TUG1 to down-regulate nucleotide binding oligomerization domain like receptor protein 1 inflammasome in delaying the progression of Alzheimer’s disease [J]. Acta Anatomica Sinica, 2024, 55(1): 32-42.
[5]	ZOU Cheng-gong FENG Hao CHEN Bing TANG Hui SHAO Chuan SUN Mou YANG Rong HE Jia-quan . Research on the dynamic changes of neurological dysfunction and cognitive function impairment in traumatic brain injury [J]. Acta Anatomica Sinica, 2024, 55(1): 43-48.
[6]	LÜ Hai-yan SHEN Xi-ya ZHAO Fu-sheng ZHU Mei . Effects of tricholoma matsutake polysaccharides on 1-methy-4-pehnyl-pyridine ion-induced PC12 cell damage [J]. Acta Anatomica Sinica, 2024, 55(1): 49-54.
[7]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[8]	HAO Yong-ming GUO Lei ZHOU Cheng-hui PEI Han-jun LI Li ZHAO Zhe . Establishment of a rat model of myocardial hypertrophy by a modified abdominal aortic coarctation method [J]. Acta Anatomica Sinica, 2024, 55(1): 120-124.
[9]	XU Ya-ping YU Yue-xin CHEN Jin-fu WANG Ke-ke GUO Zhikun . Structural distribution and mechanism of elastic fibers and collagen fibers in ventricular interstitium of aged rats [J]. Acta Anatomica Sinica, 2023, 54(6): 716-721.
[10]	WANG Wen-juan DING Yu-tong SU Hao REN Jie SUN Yan-rong WANG Han-fei LU Jia-li ZHANG Lin-qian BAI Yu QIN Li-hua. Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition [J]. Acta Anatomica Sinica, 2023, 54(6): 620-627.
[11]	ZHANG Qin YANG Jun-xia JIANG Qing-song. Effect of fosinopril on angiotensin converting enzyme 2 and vascular endothelial growth factor in diabetic retinopathy mice [J]. Acta Anatomica Sinica, 2023, 54(6): 628-634.
[12]	CHEN Zi-xuan SI Li-na SHU Wei-han ZHANG Xin WEI Chen-yang CHENG Lu-yang YANG Song-he QIAO Yue-bing. Mechanism of melatonin regulation of PI3K/Akt/mTOR signaling in hypothalamus delaying the initiation of puberty in female mice mice [J]. Acta Anatomica Sinica, 2023, 54(6): 644-651.
[13]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.
[14]	XU Ying LIU Bin ZHENG Xing HE Zong-zhao. Role and mechanism of complement C3a receptor in the cognitive impairment of septic rats induced by cecal ligation and perforation [J]. Acta Anatomica Sinica, 2023, 54(6): 668-675.
[15]	LIU Zhe-chuan LI Kun MA Shuai-nan MENG Jia-qi WANG Yan-qin. Effects of liraglutide on inflammation and mitochondrial fusion/division in Parkinson’s disease model of mice induced by paraquat [J]. Acta Anatomica Sinica, 2023, 54(6): 676-681.