Abstract:

Objective To investigate the effects of 3-N-butylphthalide (NBP) pretreatment on the score of neurological deficit, oxidative stress and pathomorphology in rats with cerebral ischemia reperfusion injury (CIRI). Methods Ninety male SD rats were randomly divided into sham operation group (Sham group), model group (IR group), NBP pretreatment low dose group (NBPⅠgroup), NBP pretreatment middle dose group(NBPⅡgroup) and NBP pretreatment high dose group(NBPⅢ group), 18 rats per group. Pretreatment was given once a day within 1 week before establishing the model of cerebral ischemia reperfusion injury. The model of middle cerebral artery occlusion(MCAO) was subjected by suture method. The score of neurological deficit was executed after ischemia for 2h and reperfusion for 24h in all the rats. The cerebral infarction was observed by TTC staining. The pathologic change of brain was observed by HE staining under the microscope. Hydroxylamine method was used to detect activity of SOD, chemical colorimetry method was used to measure activity of GSH-PX, and TBA method was used to detect content of MDA.
Results (1) In Sham group, the score of neurological deficit and the percentage of infarction volume were zero, the morphology of nerve cell was regular, and activity of SOD, GSH-PX and content of MDA of brain tissue were normal. (2) Compared with IR group, the score of neurological deficit was significantly reduced in NBP pretreatment groups (all P＜0.01); the score of neurological deficit was decreased progressively in turn in NBP Ⅰ,Ⅱ,Ⅲ group (all P＜0.05). (3) Compared with IR group, the percentage of infarction volume was cut down progressively in turn in NBPⅠ,Ⅱ,Ⅲ group (all P＜0.05), and neuron injury was also induced obviously in NBP pretreatment groups.(4) Activity of SOD, GSH-PX was largely increased, and content of MDA was greatly decreased in NBP pretreatment groups(P＜0.01). Activity of SOD, GSH-PX went up progressively in turn, and contents of MDA were cut down progressively in turn in NBP Ⅰ,Ⅱ,Ⅲgroup (all P＜0.05). Conclusion 3-N-butylphthalide can significantly up-regulate the activity of SOD and GSH-PX, decrease the content of MDA, reduce the percentage of infarction volume, and relieve the damage of nerve cell to preventively protect the rats with cerebral ischemia reperfusion injury.

Key words: 3-N-butylphthalide, Pretreatment, Cerebral ischemia reperfusion, Oxidative stress, HE staining, Rat