Effect of annexin A7 knockdown on biological behaviour of MGC-803 cells

doi:10.16098/j.issn.0529-1356.2015.02.012

Abstract

Abstract:

Objective To explore the effect of annexin A7(ANXA7) knockdown on biological behaviour of MGC-803 cells. Methods The MGC-803 cells were grouped into experiment group, negative group and blank group. RNA interference technology was used to transfect the siRNA targeted ANXA7, scrambled siRNA into MGC-803 cells with lipofectine 2000 without any treatment to the blank group. After transfection for about 48 hours, Western blotting and Real-time PCR were used to assure the suppression of ANXA7 on both protein level and mRNA level. Cell adhesion assay, detachment assay, and spreading assay and wound healing assay were used to detect the adhesion, detachment, spreading and migration abilities of MGC-803 cells. Results The expression of ANXA7 was significantly suppressed in the cells which were transfected with ANXA7 siRNA. When ANXA7 expression was suppressed, the cell adhesion rate, detachment rate, spreading rate in experiment group were significantly lower than those in negative group and blank group(P<0.05). Compared with the negative group and blank group, migration ability of experiment group was not significantly different. Conclusion The biological behaviour of MGC-803 cells change obviously when ANXA7 expression is suppressed and ANXA7 could become potential target of therapeutic strategies.

Key words: Annexin A7, Cell motility, MGC-803 cell, Western blotting

TIAN Huan-na WANG Xiao-jie CHEN Long LIANG Xiu-jun LIU Lan-fang LI Xin*. Effect of annexin A7 knockdown on biological behaviour of MGC-803 cells[J]. AAS, 2015, 46(2): 216-220.

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