Expression of annexin A7 in human gastric carcinomas and the clinic significance

doi:10.16098/j.issn.0529-1356.2015.04.014

Abstract

Abstract:

Objective To dectect the expression of annexin A7 in human gastric carcinoma so as to detect the mechanism. Methods Fifty five gastric carcinoma samples and 25 normal human gastric samples were collected. Each sample was lysed in lysis buffer. Whole protein of the supernatant was estimated by BCA-100 Protein Quantitative Analysis Kit. The expression of annexin A7 in human gastric carcinoma and normal gastric tissue was examined with Western blotting and Real\|time PCR. Immunehistolchemistry was used to detect the expression position of annexin A7 in the gastric carcinoma cells. Results The expression of annexin A7 increased in gastric cancer not only on protein level but also on RNA level when compared with normal gastric tissues, the difference was significant (P<0.05); Annexin A7 mainly expressed in the cytoplasm of the gastric carcinoma cells. Conclusion Expression of annexin A7 is up-regulated in human gastric cancer, and it may involve in the development of gastric cancer.

Key words: Annexin A7, Gastric cancer, Western blotting, Real-time PCR, Human

HANG Yang XU Qian CHEN Long WANG Xiao-jie TIAN Huan-na LI Chun-hui LI Xin*. Expression of annexin A7 in human gastric carcinomas and the clinic significance[J]. AAS, 2015, 46(4): 528-531.

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