Expression of gene associated with retinoic-interferon-induced mortality-19 and its effect on mouse embryo implantation

doi:10.16098/j.issn.0529-1356.2015.04.006

Abstract

Abstract:

Objective To explore the expression of gene associated with retinoic-interferon-induced mortality-19(GRIM-19) gene and the effect of GRLM-19 on the embryonic implantation. Methods The expression of GRIM-19 in the pregnant mouse uterus was detected by immunohistochemistry. The expression of GRIM-19 in the mouse uterus was detected by Real-time PCR and Western blotting with early pregnancy,hormone treatment and pseudopregnancy models. TUNEL method was used to detect apoptosis in uterine tissues of pregnant mice. An optimized model of R95-2 spheroids and a Bewo monolayer co-culture model were employed to study the effect of GRIM-19 in the embryo-endometrial epithelium attachment. Apoptosis was assayed by flow cytometry. Mitochondrial membrane potential was assayed by JC-1. Western blotting and Real-time PCR were used to detect signal transducer and activator of transcription(STAT3), tumor necrosis factor(TNF)-α and interleukin (IL)-11 mRNA and protein level. Results The immunohistochemical positive staining for GRIM-19 was distributed in the luminal epithelium and glandular epithelium in pregnant mice. The intensities of both GRIM-19 protein and mRNA positive staining were decreased to the lowest level at pregnancy day 4 in the pregnancy group, no significant difference in the pseudopregnancy group, and lower in the estrogen treatment group. Overexpression of GRIM-19 made RL95-2-BeWo co-culture spheroid adhesion rate decreased, apoptosis increased, and mitochondrial membrane potential reduced; STAT3 and IL-11 mRNA and protein levels reduced, but TNF-α protein and mRNA levels increased. Conclusion GRIM-19 plays an important role in embryo implantation, possibly through the regulation of apoptosis and immune tolerance to provide protection for the embryo implantation.

Key words: GRIM-19, Embryo, Implantation, Uterus, Immunohistochemistry, Rat

SUN Yan-yan DENG Xiao-hui CHAO Lan* YANG Yang CHENG Lai-yang DU Tiao-qi WANG Jing. Expression of gene associated with retinoic-interferon-induced mortality-19 and its effect on mouse embryo implantation[J]. AAS, 2015, 46(4): 471-479.

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