Age-related changes in the total number of muscle fibers,cross-sectional areas of the muscle and slow and fast muscle fibers in the rat soleus muscle

doi:10.16098/j.issn.0529-1356.2015.05.015

Abstract

Abstract:

Objective To investigate morphological changes of the soleus muscle of SD rats with various ages. Methods The shape and total number of muscle fibers, and crosssectional area (CSA) the soleus muscle of 4-, 18-, 25- and 30-month old male SD rats (5 muscles in each age group) as well as the distributional characteristics and CSA of slow and fast muscle fibers were observed and measured using hematoxylin and eosin staining, succinate dehydrogenase activity assay and immunohistochemistry. Results The shape of muscle fibers in the soleus became irregular with advancing age, combined with splitting and grouping of muscle fibers. The total number of muscle fibers in 30-month soleus reduced significantly compared with that of 4-month muscle (P＜0.05) and the CSA of 30-month soleus also decreased in comparison with that of the other age groups (P＜0.01). The CSA of slow muscle between 4-month and 25-month and the CSA of fast muscle fibers between 18-month and 25-month groups showed no difference (P＞0.05). Conclusion The total number of muscle fibers in soleus and CSA of soleus, slow and fast muscle fibers in soleus in 30month SD rat significantly decline compared with those at the other ages. It probably implies that the serious dysfunctional changes may happen to skeletal muscle in the late stage of aging.

Key words:

Soleus, Slow muscle fiber, Fast muscle fiber, Total number, Cross-sectional area, Immunohistochemistry, Rat

WANG Ling-zhan* WANG Li-qun MENG Zhuang-zhi WANG Xiao-ping ZHANG Li-wei LI Di LIU Ming-jing. Age-related changes in the total number of muscle fibers,cross-sectional areas of the muscle and slow and fast muscle fibers in the rat soleus muscle[J]. AAS, 2015, 46(5): 660-666.

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