Morphology and protein labeling of telocytes in cardiac neonatal rats

doi:10.16098/j.issn.0529-1356.2016.05.009

Abstract

Abstract:

Objective To study the morphological characteristics of telocytes（TCs）in neonatal rats and to verify whether TCs express the special markers of neural cells and glial cells. Methods Cardiac TCs were isolated and cultured from neonatal rats with collagenase digestion method. Immunocytochemistry, immunofluorescence and scanning electron microscopy were used to identify TCs, and to detect whether TCs had characteristics of neural and glial cells. Neonatal rat brain tissue was used as a positive control. Results Cardiac TCs were in a good state and of high purify. The immunocytology staining showed that cultured cells positively expressed vimentin and co-expressed vimentin and CD34 rather than neuron-specific enolase(NSE), glia fiber acidic protein(GFAP), OX42 and 2’3’-cyclic nucleotide 3’-phosphodiesterase (CNpase). The morphology of TCs was clear and distinguishable under a scanning electron microscope. Conclusion Cardiac TCs do not have the characteristics of neuronal and glial cells though they have some similar morphology.

Key words: Telocyte, Nerve cell, Glial cell, Primary cell culture, Neonatal rat

GAN Li CHANG Yu-qiao ZHANG San-lin NIU Yan-fei YU Xia GUO Zhi-kun. Morphology and protein labeling of telocytes in cardiac neonatal rats[J]. Acta Anatomica Sinica, 2016, 47(5): 633-637.

References

［1］Gherghiceanu M,Manole CG,Popescu LM,et al.Telocytes in endocardium:electron microscope evidence［J］.J Cell Mol Med, 2010,14(9):2330-2334.

［2］Popescu LM,Manole CG,Gherghiceanu M,et al.Telocytes in human epicardium［J］.J Cell Mol Med,2011,14(8):2085-2093.

［3］Popescu LM,Gherghiceanu M,Suciu LC,et al.Telocytes and putative stem cells in the lungs：electron microscopy,electron tomography and laser scanning microscopy［J］.Cell Tissue Res,2011,345(3):391-403.

［4］Rusu MC,Jianu AM,Mirancea N,et al.Tracheal telocytes［J］.J Cell Mol Med,2012,16(2)：40l-405.

［5］Qi G,Lin M,Xu M,et al.Telocytes in the human kidney cortex ［J］. J Cell Mol Med, 2012,16(12):3116-3122.

［6］Suciu L,Popescu LM,Gherghiceanu M,et al.Telocytes in human term placenta:morphology andpheno type［J］.Cells Tissues Organs,2010,192(5):325-339.

［7］Rusu MC,Mirancea N,Mnoiu VS, et al.Skin telocytes［J］.Ann Anat,2012,194(4):359-367.

［8］Popescu LM,Manole E,Serboiu CS,et al.Identification of telocytes in skeletal muscle interstitium:implication for muscle regeneration［J］.J Cell Mol Med,2011,15(6):1379-1392.

［9］Popescu LM,Faussone-P ellegrini MS.Telocytes-a case of serendipity:the winding way from interstitial cells of Cajal(ICC).via interstitial Cajal-like cells(ICLC)to telocytes［J］.J Cell Mol Med,2010,14(4):729-740.

［10］Beltrami AP,Barlucchi L,Torella D,et al. Adult cardiac stem cells are multipotent and support myocardial regeneration ［J］. Cell,2003,114(6) :763-776.

［11］Suciu L,Popescu LM,Gherghiceanu M,et al.Telocytes in human term placenta:morphology and phenotype ［J］.Cells Tissues Organs,2010,192(5):325-339.

［12］Manole CG,Cismasiu V,Gherghiceanu M,et al.Experimental acute myocardial infarction：teloeytes involvement in neoangiogenesis ［J］.J Cell Mol Med,2011,15(11):2284-2296.

［13］Manole CG, Cisma?瘙塂iu V, Gherghiceanu M,et al.Experimental acute myocardial infarction: telocytes involvement in neo-angiogenesis［J］. J Cell Mol Med, 2011, 15(11): 2284-2296.

［14］ChangY,Li C,Guo Z,et al. Multiple immunophenotypes of cardiac telocytes ［J］.Exp Cell Res,2015,338(2):239-244.

［15］Chang YQ,Li CX,Guo ZhK,et al. Division mode of cultured rat cardiac telocytes［J］. Acta Anatomica Sinica,2015,46(5):629-633.(in Chinese)

常玉巧，李辞霞，郭志坤，等. 新生大鼠心 telocytes在体外培养状态下的分裂方式［J］. 解剖学报，2015,46(5):629-633.

［16］Faussone Pellegrini MS,Cortesini C,Romagnoli P.Ultrastructure of the tunica muscularis of the cardial portion of the human esophagus and stomach,with special reference to the so-called Cajal’s interstitial cells ［J］.Arch Ital Anat Embriol,1977,82(2):157-177.

［17］Gherghiceanu M, Popescu LM. Cardiac telocytes-their junctions and functional implications ［J］. Cell Tissue Res,2012,384(2):265-279.

［18］Suciu L，Nicolescu MI,Popscue LM. Cardiac telocytes: serial dynamic images in cell culture ［J］.J Cell Mol Med,2010,14(11):2687-2692.

［19］Zhao B, Chen S, Liu J,et al. Cardiac telocytes were decreased during myocardial infarction and their therapeutic effects for ischaemic heart in rat ［J］. J Cell Mol Med,2013,17(1):123-133.

［20］Yonghua Z, Chunxue B, Xiangdong W.Telocyte morphologies and potential roles in diseases［J］.J Cell Physiol, 2012, 227(6):2311-2317.

［21］Popescu LM, Gherghiceanu M, Manole CG, et al. Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocytes progenitors in epicardial stem cell niches ［J］. J Cell Mol Med, 2009,13(5):866-886.

[1]	BIAN Wei LI Meng-yi ZHOU Peng LI Jun-wei ZHANG Ting WU An-ting QI Shuang-shuang CUI Huai-rui SUN Chen-you. Effects of activating mTORC2/Akt signaling pathway on dopaminergic neurons and behaviors in 6-hydroxydopamin model mice [J]. Acta Anatomica Sinica, 2023, 54(1): 13-22.
[2]	HUANG Jing-wen ZHANG Ren-fei JIAN Xiao-ting LIAO Zhao-hong LAN Hai-qiang HUANG Tao HU Ji-jie LIAO Hua. Regulation effect of endoplasmic reticulum stress and unfolded protein response activation on skeletal muscle fiber immune behavior [J]. Acta Anatomica Sinica, 2022, 53(6): 727-736.
[3]	XU Hong-bei LUO Yong. Triggering receptor expressed on myeloid cell-2 modulating the polarization of mouse M2 microglia with oxygen-glucose deprivation/re-oxygenation model [J]. Acta Anatomica Sinica, 2021, 52(3): 329-336.
[4]	ZOU Lin-qing YI Xin HE Hui SHAN Bo-quan JIN Guo-hua LI Hao-ming. Expression of RUNX1T1 and neuronal differentiation in radial glial cells after deafferented injury of hippocampus in vitro [J]. Acta Anatomica Sinica, 2020, 51(6): 809-814.
[5]	LIN Ren LUO Li-li LI Qiu-hua CHEN Ting QIU Rong-hui LUO Dao-shu. Effect of chronic compression injury of trigeminal nerve root on expression of myelin basic protein and P75 in trigeminal ganglion cells [J]. Acta Anatomica Sinica, 2019, 50(6): 707-712.
[6]	LI Mei-yu MU Jiao WANG Ting-ting LI Wei-min ZHANG Guo-hui. Glial response following diffuse axonal injury [J]. Acta Anatomica Sinica, 2019, 50(5): 554-560.
[7]	PENG Li-juan HUANG Yue YAN Hui-wen GAO Jie HU Rong SU Min. Imatinib mesylate regulating the effect of Telocytes on salivary secretion of submandibular gland [J]. Acta Anatomica Sinica, 2019, 50(1): 91-97.
[8]	CHANG Yu-qiao LI Ci-xia JIA Yang-yang GUO Zhi-kun. Multiple phenotypes in cardiac fibroblasts [J]. Acta Anatomica Sinica, 2018, 49(3): 317-323.
[9]	DONG Chuang WANG Hao. Effects of intravenous anesthetics on lipopolysaccharide-induced proliferation of glial cells and its mechanisms [J]. Acta Anatomica Sinica, 2018, 49(3): 299-302.
[10]	ZHU Zhan-zhan HOU Bin SUN Wen-wen YUAN Hui-fang ZHANG Yong-chun CAI Xin-hua. Cardiac fibroblasts of the rat transfected by lenti virus-stromal cell-derived factor-1α-green fluorescent protein vector [J]. Acta Anatomica Sinica, 2017, 48(1): 43-47.
[11]	XU Sha-sha LIAO Zhao-fu CAI Dong-qing. Distribution of cardiac telocytes in the aged rat heart [J]. Acta Anatomica Sinica, 2016, 47(6): 802-806.
[12]	CUI Zhan-jun BIANKe ZHAO Kai-bing SHI Shu-qin DENG Jin-bo*. Relationship between nerve cells, glial cells and blood vessels during the development of mouse cerebral cortex [J]. AAS, 2016, (2): 152-158.
[13]	CHANG Yu-qiao LI Ci-xia GUO Zhi-kun* LI He*GAN Li NIU Yan-fei. Division mode of cultured rat cardiac telocytes [J]. AAS, 2015, 46(5): 629-633.
[14]	LU Zhao-hui CHANG Yu-qiao GUO Zhi-kun* ZHAO Guo-an LI Qiong LI Ci-xia. Expression of nanog protein in fibroblasts from multiple organs of the neonatal rat [J]. AAS, 2015, 46(4): 488-494.
[15]	QIN Jian-bing CHENG Min JIN Guo-hua* LI Hao-ming SHI Jin-hong ZOU Lin-qing TIAN Mei-ling. Effects of Jagged1 on hippocampal radial glial cells’proliferation and neuronal differentiation [J]. AAS, 2014, 45(5): 585-590.