Effects of intravenous anesthetics on lipopolysaccharide-induced proliferation of glial cells and its mechanisms

doi:10.16098/j.issn.0529-1356.2018.03.005

Abstract

Abstract:

Objective To detect the effect and mechanism of intravenous anesthetics on lipopolysaccharide (LPS) treated glial cells. Methods The proliferative ratio and the apoptotic ratio of glial cells after treated with LPS were detected by MTT assay, colony formation assay, and Annexin V-FITC/PI double staining, respectively. The protein related to the mechanisms was analyzed by Western blotting. Results Intravenous anesthetics reversed the LPS-induced proliferation of glial cells. The levels of p-Akt and p-mTOR were downregulated, while the levels of Caspase-3 and Caspase-9 were upregulated in intravenous anesthetics treated cells than untreated ones. Conclusion Intravenous anesthetics may reverse LPS-induced proliferation of glial cells by suppressing mTOR signaling pathway.

Key words: Glial cell, Lipopolysaccharide, mTOR, Western blotting, Rat

DONG Chuang WANG Hao. Effects of intravenous anesthetics on lipopolysaccharide-induced proliferation of glial cells and its mechanisms[J]. Acta Anatomica Sinica, 2018, 49(3): 299-302.

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