Abstract:

Objective To establish an OSKM induced C3A-c-Jun of liver cancer reprogrammed cell line, and to explore the effects of exogenous c-Jun on liver cancer cell reprogramming. Methods OSKM was utilized to induce C3A-c-Jun to reprogram. Alkaline phosphatase staining, Real-time fluorescent quantitative polymerase chain reaction(Real-time PCR), Western blotting, and immunofluorescence technology were used to identify cells, and establish the C3A-c-Jun-iCSCs. Results C3A-c-Jun-iCSCs cells formed dome-shaped clones, in which cells were small, closely packed and multi-layered. Alkaline phosphatase staining was positive. They expressed pluripotent markers Oct4, Sox2, Nanog at the mRNA level and express OCT4, and SOX2 at the protein level. C3A-c-Jun-iCSCs had obviously higher gene expression of Sox2 compared with C3A-iCSCs, whatever in exogenous expression or endogenous expression. C-Jun continually expressed in the process of reprogramming. Conclusion OSKM induces C3A/C3A-c-Jun of liver cancer reprogramming and built the C3A-i-CSCs/C3A-c-J-un-iCSCs. Exogenous c-Jun has a promoting effect on liver cancer cell reprogramming process by upregulating Sox2, which then can activate downloaded genes.

Key words: c-Jun, Induce, Cancer stem cell, Reprogramming, Western blotting, C3A cell