Effect of repulsive guidance molecule a specific inhibitor 6FNⅢ on cortex neuron autophagy in rats after cerebral ischemia/reperfusion injury

doi:10.16098/j.issn.0529-1356.2017.04.003

Abstract

Abstract:

Objective To determine the effect of repulsive guidance molecule a (RGMa) specific inhibitor 6FNⅢ on cerebral ischemia/reperfusion(I/R) injury-induced autophagy and neurological function. Methods Different concentrations of 6FNⅢ were injected into the right lateral ventricles of adult male Sprague-Dawley rats before I/R surgery. The most proper concentration of 6FNⅢ was assessed by Western blotting. The rats were randomly divided into sham operation group（sham）,cerebral I/R group（I/R）,I/R plus normal saline group（I/R+NS）,I/R plus 6FNⅢ intervention group（I/R+6FNⅢ）, 18 rats per group. NS and 6FNⅢ were injected into the right lateral ventricle of the rats before I/R surgery. The blood was occluded for 120 minutes, followed by 24 hours reperfusion. Neurological function was evaluated. The expression of LC3Ⅱ/Ⅰ in the brain tissue was detected by Western blotting and immunohistochemical assay. Transmission electron microscope was used to observe the autophagosome formation. Results According to the expression of CRMP-2 , the optimum concentration of 6FNⅢ was 0.5 g/L. 6FNⅢ treatment improved neurological deficits(P<0.05), decreased LC3Ⅱ/Ⅰ levels(P<0.05), and attenuated autophagosome formation at 24 hours after cerebral I/R injury. Conclusion RGMa-specific inhibitor 6FNⅢ may protect the brain against strokeinduced injury through inhibiting autophagy.

Key words: Repulsive guidance molecule a, 6FNⅢ, Cerebral ischemia/reperfusion, Autophagy, Western blotting, Rat

XIE Fei QIN Xin-yue ZHANG Rong-rong LI Min.

Effect of repulsive guidance molecule a specific inhibitor 6FNⅢ on cortex neuron autophagy in rats after cerebral ischemia/reperfusion injury

[J]. Acta Anatomica Sinica, 2017, 48(4): 381-386.

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