Nitrite exposure and DNA methylation and histone deacetylation in mouse brain

doi:10.16098/j.issn.0529-1356.2017.06.002

Abstract

Abstract:

Objective To investigate the inflammatory damage in the mouse neocortex after nitrite exposure, and to understand the regulation mechanism of DNA methylation and histone deacetylation. Methods C57BL/6 J adult male mice were used to establish the models of prenatal nitrite exposure, wherefore control group (physiological saline), low dose nitrite exposure group (3 g/L) and high dose nitrite exposure group (6 g/L) were included. Immunocytochemistry and Western blotting were carried out to analyze the inflammatory damage, histone deacetylation and DNA methylation in the neocortex. Results The expressions of cyclooxygenase 2(COX2), interleukin-1β(IL-1β), ionized calcium binding adapor molecule-1(Iba1), c-Fos and IL-6 were significantly more than those in the control group (P<0.01) after chronic nitrite exposure. The expression of 5-methylcytosine(5-mC), DNA methyctrasferace 1(DNMT1), DNA DNMT3a and histone deacetylase 1(HDAC1) in treatment groups were significantly lower than that in the control group (P<0.01) with dose dependency. Conclusion Nitrite exposure can induce cell immune inflammatory damage in the neocortex of mice, and DNA methylation and histone deacetylation may be involved in regulation of immune inflammatory reaction after nitrite exposure.

Key words: Nitrite exposure, Inflammatory reaction, DNA methylation, Histone deacetylation, Western blotting, Mouse

LIU Jun ZHANG Li-li SHI Zhen-yu DENG Jin-bo. Nitrite exposure and DNA methylation and histone deacetylation in mouse brain[J]. Acta Anatomica Sinica, 2017, 48(6): 642-650.

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