MicroRNA-539 inhibiting the occurrence and development of hepatocellular carcinoma by targeting E2F transcription factor 3

doi:10.16098/j.issn.0529-1356.2018.04.009

Abstract

Abstract:

Objective To investigate the expression of microRNA-539 (miR-539) in hepatocellular carcinoma (HCC) and the possible mechanism of miR-539 inhibition cancer cells. Methods Ninety cases of liver cancer patients after liver cancer tissue and adjacent tissue specimens were collected, and the expression of miR-539 in clinical tissues was detected by Real-time PCR. MiR-539 mimics was transfected into QGY-7701, Huh7 and PCL/PRF5 hepatoma cells, and the expression of miR-539 was detected by Real-time PCR. The effect of miR-539 on the proliferation of HCC cells was detected by MTT assay. The effect of miR-539 on the apoptosis of hepatocellular carcinoma cells was detected by flow cytometry. The effect of miR-539 on the expression of E2F transcription factor 3(E2F3) protein in hepatocarcinoma cells was detected by Western blotting. Results The expression of miR-539 in hepatocellular carcinoma was significantly lower than that in adjacent tissues. The expression of miR-539 was not correlated with sex, age and histological differentiation of hepatocellular carcinoma (P>0.05). The expression of miR-539 was correlated with tumor diameter and lymph node metastasis (P<0.05). The expression of miR-539 in miR-539 mimics group was significantly higher than that in blank group and miR-539 NC group (P<0.05). In QGY-7701 cells, overexpression of miR-539 significantly inhibited cell proliferation (P<0.05), decreased E2F3 protein expression (P<0.05), but was no significant effect on apoptosis (P>0.05). Conclusion miR-539 is a tumor suppressor factor in the development of hepatocellular carcinoma, which may inhibit the proliferation of cancer cells through the down-regulation of E2F3 protein level.

Key words: Hepatocellular carcinoma, microRNA-539, Transcription factor, Proliferation, Real-time PCR, Western blotting, Human

DENG Xue-jun GONG Shao-xin YANG Sheng-hui HUANG Li-rong ZHOU Fang. MicroRNA-539 inhibiting the occurrence and development of hepatocellular carcinoma by targeting E2F transcription factor 3[J]. Acta Anatomica Sinica, 2018, 49(4): 469-474.

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