Effects of vasoactive intestinal peptide on synapse of MPTP-induced mice with Parkinson’s disease

doi:10.16098/j.issn.0529-1356.2018.01.005

Abstract

Abstract:

Objective To study the effects of vasoactive intestinal peptide (VIP) on synaptic and behavioral alterations in the mice model of Parkinson’s disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6tetrahydropyridine (MPTP). Methods Thirty male C57BL/6 J mice were randomly divided into normal saline (NS) group, MPTP group and MPTP + VIP group. The behavioral changes of mice were measured by the Tru Scan system. The expression of tyrosine hydroxylase (TH) in the substantia nigra (SN) and striatum and vesicle-associated membrane protein 2 (VAMP2) and synapsin 1 (SYN1) in the striatum were examined by immunohistochemistry, and the levels of VAMP2 and SYN1 were analyzed by Western blotting. The synaptic structural changes in the SN were observed by transmission electron microscopy. Results In the behavioral test, the MPTP group had a significantly lower vertical movement distance than that of the control group, while the MPTP group was markedly higher than that of MPTP group. Compared with the MPTP group, VIP treatment obviously increased the average absorbance (AA) of TH, VAMP2 and SYN1 immunolabeling in striatum and the number of dopaminergic neurons in the SN, while the levels of the VAMP2 and SYN1 proteins also elevated. Synapse density in the model group was less than that in the control group. The amount of synaptic vesicles was decreased in the model group compared to the control, but normalized in the MPTP + VIP group. Conclusion VIP administration can up-regulate the expression of VAMP2 and SYN1, mitigate synaptic damage, protect dopaminergic neurons and improve movement in the MPTP model mice.

Key words: Parkinson’s disease, Vasoactive intestinal peptide, Vesicle-associated membrane protein 2, Synapsin 1, Western blotting, Transmission electron microscopy, Mouse

CLC Number:

Ｒ742. 5

Lü E FEI Xue-chao LI Kan ZHAN Tong-xia LI Feng-jie FU Wen-yu. Effects of vasoactive intestinal peptide on synapse of MPTP-induced mice with Parkinson’s disease[J]. Acta Anatomica Sinica, 2018, 49(1): 29-34.

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