Kinesin1-mediated neuronal axoplasmic transport disorder in cerebral cortex of amyloid precursor protein/presenilin-1 transgenic mice

doi:10.16098/j.issn.0529-1356.2018.02.004

Abstract

Abstract:

Objective To understand the roles of axonal transport proteins, kinesin1 and SIM-312, in the pathogenesis and development of Alzheimer’s disease. Methods Amyloid precursor protein（APP）/ presenilin-1（PS1）transgenic mice (n=40) and wild type mice (n=40) from postnatal day 30 to postnatal day 360 were used in the study. Amyloid beta-peptides(Aβ）plaques and astrocytes in cerebral cortex were visualized with immunofluorescent labeling and Western blotting. Results Compared with wild type mice, Aβ plaques and astrocytes increased in cerebral cortex of APP/PS1 mutant mice, especially after 9 months, with statistical significance. However, after 9 months, the number of kinesin1-positive cells decreased in APP/PS1 transgenic mice, and SIM-312 positive neurofilaments appeared tangled in the cerebral cortex of AD mice as well. Conclusion Abnormal kinesin1 and SIM-312 are involved in axonal transportation disorder in AD mice, suggesting they are the important pathogenic factors of AD.

Key words: Alzheimer's disease, Axial transport barrier, Neurofilament, Kinesin1, Western blotting, Mouse

WANG Qian FAN Wen-juan SUN Yi-zheng WANG Lai DENG Jin-bo.

Kinesin1-mediated neuronal axoplasmic transport disorder in cerebral cortex of amyloid precursor protein/presenilin-1 transgenic mice

[J]. Acta Anatomica Sinica, 2018, 49(2): 158-165.

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