Apoptosis of olfactory bulb cells in MPTP cynomolgus monkey model of Parkinson’s dise

doi:10.16098/j.issn.0529-1356.2019.01.001

Abstract

Abstract:

Objective To explore the apoptosis and the proliferation of glia cells in olfactory bulb of cynomolgus monkeys damaged by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Methods Three adult cynomolgus monkeys were injected with MPTP to induce the damage of olfactory bulb as MPTP group (model group) and other three adult cynomolgus monkeys as saline group (control group). Immunohistochemical staining was performed to examine the expression of Caspase-3, Bcl-2, ionized calcium binding adaptor molecule-1(Iba-1) and glial fibrillary acidic protein(GFAP) in the olfactory bulb. The numbers of Caspase-3, Bcl-2, Iba1 and GFAP-positive cells were counted and the average absorbance was measured by Image J v1.8.0 in control and MPTP group. Results Compared with the normal control group, Caspase-3-positive cells increased significantly in the glomerular layer (GL) of olfactory bulb, and Bcl-2-positive cells decreased significantly in MPTP group after MPTP injury, and GFAP-positive and Iba-1-positive cells were located in GL and external plexiform layers (EPL). The number of GFAP-positive and Iba-1-positive cells increased in olfactory bulb in the MPTP group. Conclusion MPTP can induce the cell apoptosis, astrocyte and microglial activation in olfactory bulb of cynomolgus monkeys, which may be associated with the dysosmia in Parkinson’s disease.

Key words: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Olfactory bulb, Immunohistochemistry, Cynomolgus monkey

CHEN Ming-yu CHENG Wei YAN Li-li GUAN Yun-qian WU Di YUE Feng REN Zhen-hua. Apoptosis of olfactory bulb cells in MPTP cynomolgus monkey model of Parkinson’s dise [J]. Acta Anatomica Sinica, 2019, 50(1): 3-7.

References

［1］Braak H, Sastre M, Bohl JR, et al. Parkinson’s disease: lesions in dorsal horn layer Ⅰ, involvement of parasympathetic and sympathetic pre-and postganglionic neurons［J］. Acta Neuropathol， 2007, 113(4): 421-429.

［2］Kadhim S, Pringsheim T, Le A, et al. Validating screening tools for depression in Parkinson’s disease［J］. Mov Disord， 2018, 33(7): 1184-1186.

［3］Cury RG, Carvalho MJ, Lasteros F, et al. Effects of subthalamic stimulation on olfactory function in Parkinson disease［J］. World Neurosurg， 2018, 114:e559-e564.

［4］Haehner A, Boesveldt S, Berendse HW, et al. Prevalence of smell loss in Parkinson’sdisease—a multicenter study［J］. Parkinsonism Relat Disord， 2009, 15(7): 490-494.

［5］Brodoehl S, Klingner C, Volk GF, et al. Decreased olfactory bulb volume in idiopathic Parkinson’s disease detected by 3.0-tesla magnetic resonance imaging［J］. Mov Disord， 2012, 27(8): 1019-1025.

［6］Kurlan R, Kim MH, Gash DM. Oral levodopa dose-response study in MPTP-induced hemiparkinsonian monkeys: assessment with a new rating scale for monkey parkinsonism［J］. Mov Disord， 1991, 6(2): 111-118.

［7］Cheng W, Ren ZhH, Guan YQ, et al. Impact on the dopaminergic neurons of olfactory bulb in cynomolgus monkeys damaged by MPTP［J］. Acta Anatomica Sinica, 2014, 45(3): 333-337. (in Chinese)

程炜，任振华，关云谦，等. 1-甲基-4-苯基-1,2,3,6-四氢吡啶对食蟹猴嗅球多巴胺能神经元的影响［J］. 解剖学报, 2014，45(3): 333-337.

［8］Tissingh G, Berendse HW, Bergmans P, et al. Loss of olfaction in de novo and treated Parkinson’s disease: possible implications for early diagnosis［J］. Mov Disord， 2001, 16(1): 41-46.

［9］Haehner A, Hummel T, Reichmann H. A clinical approach towards smell loss in Parkinson’s disease［J］. J Parkinsons Dis， 2014, 4(2): 189-195.

［10］Sharifi H, Mohajjel NA, Farajnia S, et al. Effect of buspirone, fluoxetine and 8-oh-dpat on striatal expression of Bax, Caspase-3 and Bcl-2 proteins in 6-hydroxydopamine-induced hemi-Parkinsonian rats［J］. Adv Pharm Bull， 2015, 5(4): 491-495.

［11］Liu Y, Guo Y, An S, et al. Targeting Caspase-3 as dual therapeutic benefits by RNAi facilitating brain-targeted nanoparticles in a rat model of Parkinson’s disease［J］. PLoS One， 2013, 8(5): e62905.

［12］Yamada M, Kida K, Amutuhaire W, et al. Gene disruption of caspase-3 prevents MPTP-induced Parkinson’s disease in mice［J］. Biochem Biophys Res Commun， 2010, 402(2): 312-318.

［13］Edlich F. BCL-2 proteins and apoptosis: recent insights and unknowns［J］. Biochem Biophys Res Commun，2018, 500(1): 26-34.

［14］Sridharan S, Mohankumar K, Jeepipalli SP, et al. Neuroprotective effect of valeriana wallichii rhizome extract against the neurotoxin MPTP in C57BL/6 mice［J］. Neurotoxicology， 2015, 51: 172-183.

［15］Ahmed Z, Shaw G, Sharma VP, et al. Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry［J］. J Histochem Cytochem，2007, 55(7): 687-700.

［16] Che Y, Hou L, Sun F, et al. Taurine protects dopaminergic neurons in a mouse Parkinson’s disease model through inhibition of microglial M1 polarization［J］. Cell Death Dis， 2018, 9(4): 435.

[1]	YUAN Lei YANG Zhi-wei YANG Hui LIU Zheng-hai HE Jie WAN Wei. Panax notoginseng saponin relieving the inflammatory pain caused by complete Freund’s adjuvant by inhibiting the activation of astrocytes in mice [J]. Acta Anatomica Sinica, 2024, 55(1): 25-31.
[2]	ZHENG Li-ping LIU Xia DU Xiao-hua WU Ya-juan LIU Shan-shan . Expression and distribution of brain-derived neurotrophic factor in different cerebrum regions of yak and cattle [J]. Acta Anatomica Sinica, 2024, 55(1): 10-16.
[3]	LIU Li-ping ZHU Meng-ni XU Hui . Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide induced preeclampsia rats [J]. Acta Anatomica Sinica, 2023, 54(6): 722-729.
[4]	LIANG Pan-pan YU Ai-mei DU Jing KOU Wen-hui WANG Huan-huan SONG Ai-xia. Inhibitory effect of sodium ferulate on inflammatory response in migraine rats based on c-Jun N-terminal kinase/p38 mitogen-activated protein kinase signaling pathway [J]. Acta Anatomica Sinica, 2023, 54(6): 652-659.
[5]	YAN Kun WU Xiao-lin LIU Ying-juan GE Ke-li1 REN Lei-ming LI Hong-yun . Effect of cerebrotein hydrolysate-Ⅰon intestinal microflora regulation of mice with Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(5): 497-504.
[6]	ZHUANG Hai-rong WU Zi-dong CHEN Xue-hong LI Cheng-jun. Repairing effect of sulodexide on diabetic retinopathy and the regulation of MAPK pathway in rats [J]. Acta Anatomica Sinica, 2023, 54(4): 392-399.
[7]	YU Hua-jing WEI Lu-yang LIU Shan-shan GUAN Cheng-jian ZHANG Zhong-tao. Role and clinical significance of MLLT1 super elongation complex subunit in the occurrence and development of hepatocellular carcinoma [J]. Acta Anatomica Sinica, 2023, 54(4): 425-433.
[8]	WANG Xiao LI Liang CUI Cheng-li CAI Zhi-ping YU Chang ZHANG Li-jia . Endurance exercise combined with intermittent cold stimulation promoting white adipose tissue browning in rats [J]. Acta Anatomica Sinica, 2023, 54(3): 348-356.
[9]	ZHANG Yi-zhou LI Sha MI Shi-xiong ZUO Hong-chun ZHANG Qian-qian LI Han-lin LEI Zi-han ZHANG Dong-ze CUI Hui-xian. Effect of estradiol regulating sortilin-related receptor A expression on hippocampal spine density and synaptic protein expression via estrogen receptor of mouse [J]. Acta Anatomica Sinica, 2023, 54(3): 261-268.
[10]	LIU Ke-ting CHEN Bin-bin SHUI Yu-lian SHI Qing-ming XIAO Li. Neuroprotective effect of exogenous hydrogen sulfide and the expression of matrix metalloproteinases-9 and Caspase-1 in mice model of Parkinson’s disease [J]. Acta Anatomica Sinica, 2023, 54(3): 289-295.
[11]	CHEN Bo-yu JIANG Jin-xiang ZHANG Jia-wei YANG Li . Alterations in dendritic length and branch and K+ -Cl - cotransporter 2 expression of the olfactory bulb in young APP/PS1 mice [J]. Acta Anatomica Sinica, 2023, 54(2): 127-133.
[12]	LIU Xia ZHENG Li-ping DU Xiao-hua WANG Yu-jiao WU Ya-juan . Expression and localization of neuroglobin in the brain tissue of Saiga antelope [J]. Acta Anatomica Sinica, 2023, 54(2): 188-194.
[13]	LI Qiao LIU Xia DU Xiao-hua MI Xiao-yu WEN Yong-qiang FANG Ying-dong . Expression and localization of vascular endothelial growth factor-B in diencephalon and brainstem related tissues of the Yak’s brain [J]. Acta Anatomica Sinica, 2023, 54(1): 30-35.
[14]	SU Xiao-yun HE Ji-ping CUI Jian-mei SHI Xian-feng MEN Jie. Effects of estradiol on depressive-like behavior of ovariectomized rats and its possible mechanism [J]. Acta Anatomica Sinica, 2023, 54(1): 36-41.
[15]	GAO Sai-hong ZHANG Xiao-liang YANG Ying-chun QIAO Hai-bing. Activation of p38 MAPK and its effect on the expression of Bax and Bcl-2 after cerebral ischemia-reperfusion in hyperlipidemia rats [J]. Acta Anatomica Sinica, 2023, 54(1): 50-55.