Inhibitory effect of oridonin on proliferation and migration of human esophageal squamous cancer cell lines KYSE-150 and KYSE-450

doi:10.16098/j.issn.0529-1356.2019.05.010

Abstract

Abstract:

Objective To explore the effect of oridonin (ORI) on proliferation, apoptosis, cell cycle and migration of esophageal squamous carcinoma cell (ESCC) lines KYSE-150 and KYSE-450. Methods The effect of ORI on the proliferation and clony formation of esophageal cancer cells were detected by MTT and colony formation assays. Flow cytometry was performed to examine the impact of ORI on cell apoptosis and cell cycle. Transwell assay was applied to detect the role of ORI on cell migration. The effect of ORI on the expression of anti-apoptotic protein Bcl-2, cell cycle inhibitory protein p21Cip1/Waf1, epithelialmesenchymal transition (EMT) related markers were examined by Western blotting. Results ORI had a significant inhibitory effect on the proliferation, migration and clone formation of KYSE-150 and KYSE-450 cells (P<0.05) in a time and dose-dependent manner. With the increase of ORI concentration, apoptosis rate and the proportion of cells in G₂/M phase increased significantly (P<0.05), and the proportion of cells in G₀/G₁ phase decreased significantly (P<0.05). Bcl-2, vimentin and β-catenin were down-regulated and p21Cip1/Waf1, E-cadherin were up-regulated after treatment of ORI on ESCC cells for 48 hours. Conclusion ORI may inhibit ESCC cell proliferation and clony formation by inducing apoptosis and resting cells in G₂/M phase, and suppress ESCC cell migration via inhibiting EMT process.

Key words: Esophageal squamous carcinoma cell, Oridonin, Proliferation, Migration, Flow cytometry, Human

HUANG Ke-ke LIU Yu-zhen CHEN Yan-min CHEN Zhi LIU Dan-hui ZHAO Bao-sheng. Inhibitory effect of oridonin on proliferation and migration of human esophageal squamous cancer cell lines KYSE-150 and KYSE-450[J]. Acta Anatomica Sinica, 2019, 50(5): 601-607.

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