Expression of anoctamin 1 in the process of myocardial fibrosis

doi:10.16098/j.issn.0529.1356.2019.02.006

Abstract

Abstract:

Objective To clarify the expression feature of anoctamin 1(ANO1) in the process of cardiac fibrosis and seek new targets for preventing it. Methods The myocardial infarction (MI) rats were prepared by coronary artery ligation. The left ventricular from MI group and the sham operation group were taken at one week (1 week) after MI. The changes of ANO1 expression in the two groups were detected by immunohistochemical staining and immunofluorescence double labeling. The expression of ANO1 in cardiac fibroblasts (CFs) was detected by immunofluorescence labeling, Real-time PCR and Western blotting. Results ANO1 exhibited high expression in MI and co-expressed with α-smooth muscle actin(α-SMA), and it was clearly expressed in the nucleus membrane and cytoplasm of CFs. The expression intensity of ANO1 was coincident with α-SMA. Furthermore, ANO1 expression increased significantly in the CFs after 48 hours culture comparing with the new isolated CFs. Conclusion The expression of ANO1 enhances markedly during the transformation of CFs into myocardial fibroblasts, suggesting that ANO1 may play an important role in the process of cardiac fibrosis.

Key words: Anoctamin1, Calcium-activated chloride channels, Cardiac fibroblasts, Myocardial fibrosis, Immunohistochemistry, Rat

TIAN Xiang-qin TAN Zhao-yang LI Xin-zhi MA Ke-tao LI Li SI Jun-qiang﹡. Expression of anoctamin 1 in the process of myocardial fibrosis [J]. Acta Anatomica Sinica, 2019, 50(2): 173-178.

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