Objective  To investigate the effect of obesity on the expression of inflammatory factors, and to explore the molecular mechanism of inflammatory factors in the adipose tissue.   Methods  Twenty Lepob/ob mice were randomly selected as the research object, and the wild type C57BL/6 J mice of the same nest and age as the control group. The body weight, fat mass, blood glucose level, glucose tolerance and insulin tolerance were measured. HE staining was performed to examine the morphological changes in the both white and brown adipose tissues. Western blots were conducted to analyze the protein expression of inducible nitric oxide synthase (iNOS), nuclear factor kappa-B (NF-κB), protein kinase B (Akt) and p-Akt. Furthermore, Real\|time PCR was performed to measure the mRNA expression of CC chemokine Iigand 2 (CCL2), CD44, colony stimulating factor 2(CSF2), glial fibrillary acidic protein (GFAP), Iba1, interleukin (IL)-1α, IL-6, IL-7, JUN and S100β in different adipose tissues.   Results  Comparing to the control group, the body weight of Lepob/ob mice increased significantly (P<0.001). Both fat mass and blood glucose level increased significantly (P<0.01, P<0.01, P<0.01), the tolerance of glucose was low(P<0.001) and insulin resistance was produced (P<0.001). Histological analysis of adipose tissue from Lepob/ob mice revealed an increased lipid accumulation and diameter of adipocytes compared to wild type littermates. The macrophage infiltration was also observed in adipose tissues. The expression of Janus kinase (JAK2), p-JAK2, iNOS, NF-κB, Akt and p-Akt in adipocytes of Lepob/ob mice increased significantly (P<0.05), and the expression of CCL2, CD44, IL-1α, IL-6, IL-7, JUN and S100β in the Lepob/ob mice increased significantly.   Conclusion  Obesity induces the significant expression of inflammatory factors in adipose tissue, which leads to disorder of cell secretion and conduction, and ultimately results in inflammatory cascade.