Objective To investigate the effects of fibroblast growth factor 2 (FGF-2) combined with tanshinoneⅡA on differentiation of rat bone marrow mesenchymal stem cells (BMSCs）into cardiomyocyte-like cells.  Methods BMSCs were isolated and cultured. The cultured cells were identified by flow cytometry, BMSCs were divided into experimental control group, FGF-2 group, tanshinoneⅡA group and the combined induction group. The activity and value of  BMSCs were detected by MTT. The Real-time PCR method was used to detect BMSCs. Expression of early myocardial transcription factors GATA-4 and Nkx2.5; Immunocytochemical staining for detection of connexin43（Cx43）and cardiac troponin-Ⅰ(cTnI);Immunofluorescence staining for detection of desmin and Tm; The expression of desmin and tropomyosin（Tm）was detected by Western blotting.  Results The cell activity and proliferation after induction were good. The expression of GATA-4 and Nkx2.5 in the induction group were stronger than that in the experimental control group, and the difference was statistically significant (P<0.05). The positive expression of Cx43 and cTnI in the induction group increased significantly. The markers of the combined group were the most obvious, and the difference was statistically significant (P<0.05). The expression of Myo-specific protein desmin and Tm in the combined induction group increased significantly, and the difference was statistically significant (P<0.05).  Conclusion Both FGF-2 and tanshinoneⅡA can promote the proliferation of BMSCs and induce the differentiation of BMSCs into cardiomyocyte-like cells. The synergistic effect of the two is better than other groups.