Expression profile of circular RNA and its regulatory effect on autophagy during rat liver regeneration

doi:10.16098/j.issn.0529-1356.2021.04.013

Abstract

Abstract:

Objective Studies have shown that circular RNA (circRNA) is a molecular sponge of microRNA(miRNA) and can be used for the treatment and diagnosis of liver diseases. Therefore, this study aims to further investigate the expression profile of circRNA and its effect on autophagy in rat liver regeneration. Methods Based on the high-throughput sequencing in regenerated liver of 0 hour, 2, 6, 12, 24, 30, 36, 72, 120 and 168 hours after 2/3 hepatectomy in 60 rats, the expression profiles of circRNAs, miRNAs and mRNA were analyzed and their relationship were predicted by miRanda aug 2010 software. Furthermore, according to gene function → miRNA function → circRNA function, circRNAs and miRNAs that regulating autophagy were obtained in liver regeneration and compting endogenous(ceRNA) network was constructed. Results There were 102 circRNAs, 42 miRNAs and 153 mRNAs associated with autophagy involved in liver regeneration. Using bioinformatics analysis, 44 types of circRNAs, 13 types of miRNAs and 17 types of mRNA were found to interact with each other, and their co-expression network was constructed. Conclusion In this study, we screened out the circRNAs, miRNAs and mRNAs related to autophagy in liver regeneration and built the interaction network map.

Key words: Autophagy, Liver regeneration, High-throughput sequencing, Rat

CLC Number:

Q954

WANG Qi-wen YAO Fen-jie YANG Zhuo-fei GUO Xue-qiang MU Meng-meng ZHOU Yun XU Cun-shuan. Expression profile of circular RNA and its regulatory effect on autophagy during rat liver regeneration[J]. Acta Anatomica Sinica, 2021, 52(4): 581-588.

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