Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#

doi:10.16098/j.issn.0529-1356.2021.05.007

Abstract

Abstract:

Objective To investigate the effect of fibroblast growth factor (FGF) on the proliferation and transdifferentiation of cardiac fibroblasts (CFs) into myofibroblasts (MFs). Methods Rat CFs were isolated and cultured, and then induced by FGF. CCK-8 was used to detect the cell activity and proliferation. Immunofluorescence and Western blotting were used to detect the expression of α smooth muscle actin (α-SMA) and collagen Ⅰ (ColⅠ). Results The expression and activation of α-SMA and ColⅠ increased with the increase of CFs culture generation. The number of CFs induced by FGF did not increased significantly; the expression of α-SMA in CFs induced by FGF1 and FGF2 decreased, and the number of activated MFs decreased. Conclusion FGF family has no effect on the proliferation of CFs, but FGF1 and FGF2 can inhibit the activation of CFs and reduce the differentiation into MFs.

Key words: Fibroblast growth factor, α-Smooth muscle actin, Myofibroblast, Cardiac fibroblast, Western blotting, Rat

CLC Number:

LIU Meng-xin SUN Chang-ye Li Duo REN Ming-fen GUO ZhI-kun. Effect and mechanism of fibroblast growth factor on proliferation and transdifferentiation of cardiac fibroblasts in rats#br#[J]. Acta Anatomica Sinica, 2021, 52(5): 712-719.

References

［1］ Virag JA, Rolle ML, Reece J, et al.Fibroblast growth factor-2 regulates myocardial infarct repair: effects on cell proliferation, scar contraction, and ventricular function［J］. Am J Pathol,2007,171(5):1431-1440.
［2］ Shimbori C, Bellaye PS, Xia J, et al. Fibroblast growth factor-1 attenuates TGF-beta1-induced lung fibrosis［J］. J Pathol,2016,240(2):197-210.
［3］ Furtado MB, Nim HT, Boyd SE, et al. View from the heart: cardiac fibroblasts in development, scarring and regeneration［J］. Development, 2016, 143(3): 387-397.
［4］ Svystonyuk DA, Ngu JMC, Mewhort HEM, et al. Fibroblast growth factor-2 regulates human cardiac myofibroblast-mediated extracellular matrix remodeling［J］. J Transl Med, 2015, 13: 147.
［5］ Rooij VE. Cardiac repair after myocardial infarction［J］. N Engl J Med, 2016, 374(1): 85-87.
［6］ Sun C, Li Y, Taylor SE, et al. HaloTag is an effective expression and solubilisation fusion partner for a range of fibroblast growth factors［J］. Peer J, 2015, 3: e1060.
［7］ Sun C, Liu M, Sun P, et al. Sulfated polysaccharides interact with fibroblast growth factors and protect from denaturation［J］. FEBS Open Bio, 2019, 9(8): 1477-1487.
［8］ Zhang JY, Yin GT, Guo ZhK. Endothelial cell differentiation potential of neonatal rat cardiac fibroblasts ［J］. Acta Anatomica Sinica, 2018,49 (4): 443-449. (in Chinese)
张俊悦,殷国田,郭志坤.新生大鼠心肌成纤维细胞的内皮细胞分化潜能［J］.解剖学报,2018,49(4):443-449.
［9］ Liu ML, Liu D, Liu YX, et al. Optimization of isolation and culture of primary myocardial fibroblasts from adult SD rats ［J］. Progress in Modern Biomedicine, 2016,16 (27): 5223-5226, 5236. (in Chinese)
刘美丽,刘丹,刘艳霞,等.成年SD大鼠心肌原代成纤维细胞分离及培养的优化方案［J］.现代生物医学进展,2016,16(27):5223-5226, 5236.
［10］ Souders CA, Bowers SL, Baudino TA. Cardiac fibroblast: the renaissance cell［J］. Cir Res, 2009,105(12):1164-1176.
［11］ Santiago JJ, Dangerfield AL, Rattan SG,et al. Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts［J］. Devel Dynam, 2010,239(6):573-584.
［12］ Deb A, Ubil E. Cardiac fibroblast in development and wound healing［J］. J Mol Cell Cardiol, 2014, 70: 47-55.
［13］ Papetti M,Shujath J,Riley KN.FGF-2 antagonizes the TGF-beta1-mediated induction of pericyte alpha-smooth muscle actin expression: a role for myf-5 and Smad-mediated signaling pathways［J］. Investig Ophthalmol Vis Sci,2003,44(11):4994-5005.
［14］ Maltseva O, Folger P, Zekaria D, et al. Fibroblast growth factor reversal of the corneal myofibroblast phenotype［J］. Investig Ophthalmol Vis Sci, 2001, 42(11): 2490-2495.
［15］ Mattey DL, Dawes PT, Nixon NB, et al. Transforming growth factor β1 and interleukin 4 induced α smooth muscle actin expression and myofibroblast-like differentiation in human synovial fibroblasts in vitro: modulation by basic fibroblast growth factor［J］. Ann Rheum Dis, 1997, 56(7): 426-431.
［16］ Cushing MC, Mariner PD, Liao JT, et al. Fibroblast growth factor represses Smad-mediated myofibroblast activation in aortic valvular interstitial cells［J］. FASEB J, 2008, 22(6): 1769-1777.
［17］ Fedak PWM, Bai L, Turnbull J, et al. Cell therapy limits myofibroblast differentiation and structural cardiac remodeling: basic fibroblast growth factor-mediated paracrine mechanism［J］. Circ Heart Fail, 2012, 5(3): 349-356.
［18］ Mewhort HEM, Turnbull JD, Meijndert HC, et al. Epicardial infarct repair with basic fibroblast growth factor-enhanced CorMatrix-ECM biomaterial attenuates postischemic cardiac remodeling［J］. J Thorac Cardior Sur, 2014, 147(5): 1650-1659.
［19］ Santiago JJ, Dangerfield AL, Rattan SG, et al. Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts［J］. Devel Dynam, 2010, 239(6): 1573-1584.
［20］ Itoh N, Ornitz DM. Fibroblast growth factors: from molecular evolution to roles in development, metabolism and disease［J］. J Biochemistry, 2011, 149(2): 121-130.
［21］ Turner N, Grose R. Fibroblast growth factor signalling: from development to cancer［J］. Nat Rev Cancer, 2010, 10(2): 116-129.
［22］ Zhang M, Pan X, Zou Q, et al. Notch3 ameliorates cardiac fibrosis after myocardial infarction by inhibiting the TGF-β1/Smad3 pathway［J］. Cardiovasc Toxicol, 2016, 16(4): 316-324.
［23］ Chen PY, Qin L, Li G, et al. Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression［J］. EMBO Mol Med, 2016, 8(7): 712-728.

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