Acta Anatomica Sinica

›› 2010, Vol. 41 ›› Issue (03): 339-343.doi: 10.3969/j.issn.0529-1356.2010.03.002

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Differentiation of bone marrow stromal cells into neurospheres induced by basic fibroblast growth factor combined with epidermal growth factor in rats

  

  1. 1.Department of Neurology, the First Hospital Affiliated, Chongqing Medical University, Chongqing 400016, China;2.Department of Neurology, Zhengzhou Central Hospital, Zhengzhou 450007, China;3.College of Bioengineer, Chongqing Institute of Technology, Chongqing 400050, China
  • Received:2009-03-13 Revised:2009-05-19 Online:2010-06-06
  • Contact: XIE Peng

Abstract: P>Objective To observe whether bone marrow stromal cells(MSCs) can be induced to form neurospheres by basic fibroblast growth factor(bFGF) combined with epidermal growth factor (EGF) in rats. Methods MSCs were cultured with whole bone morrow. Effect of bFGF and EGF, inducing MSCs to differentiated into neurospheres was studied by morphology, immunofluorescence, and Western blotting. Results MSCs can be effectively induced to form neurospheres by bFGF and EGF.The neurospheres can passage and differentiated into neural cells.Immunofluorescence and Western blotting showed these neurospheres expressed nestin protein. Cells differentiated from neurospheres expressed marker proteins of neurons, glial cells and oligodendrocytes. Conclusion The results indicate that MSCs can be effectively induced to form neurospheres by bFGF and EGF. The neurospheres may amplify and differentiate into neurons, glial cells and oligodendrocytes./P>

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