Abstract: Objective To observe the survival and migration of olfactory ensheathing cells (OECs) after transplanted into amyloid precursor protein (APP) transgenic mice and investigate its therapeutical effect on Alzheimer disease. Methods OECs from olfactory ensheathing bulb of enhanced green fluorescent protein(EGFP) transgenic mice were isolated, then transplanted into an APP transgenic mice. Stereotaxic coordinates and anterior-posterior were appilicated for microinjection. Confocal microscope was used to observe the survival and migration of the cells, immunohistochemistry and Western blotting were carried out to survey amyloid burden, 4 mice in each group. Results The results showed that OECs survived well and migrated away from injection site after transplanted into APP transgenic mice, the vascular phenomena of OECs was also observed, and the therapy reduced amyloid burden of Alzheimers disease model.Quantitative test showed that APP reduced significantly（EM>P/EM><0.05）. Conclusion Olfactory ensheathing cells do some help to reduce the amyloid burden in the brain of APP transgenic mice.

Key words: Alzheimer disease, β-amyloid protein, Cell transplantation, Western blotting, Amyloid precursor protein transgenic mouse