Table of Content

2021, Volume 52 Issue 1
06 February 2021 Previous Issue    Next Issue
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Neurobiology
Technology and Methodology
Review
Medical Education
Histology,Embryology and Developmental Biology
Cancer Biology
Anatomy


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Neurobiology

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Allopregnanolone protecting cell line SH-SY5Y against  6-hydroxydopamine induced lesion WANG Tong-tong YE Xin BIAN Wei CHEN Zhi-chi DU Juan-juan FU Wei-da CHEN Meng-jiao LI Jun-nan SUN Chen-you 2021, 52 (1):  5-13.  doi: 10.16098/j.issn.0529-1356.2021.01.001 Abstract ( )   PDF (10450KB) ( )   Objective To clarify the protective effect of allopregnanolone (APα) on cell line SH-SY5Y damaged by 6-hydroxydopamine (6-OHDA) and its possible molecular mechanism.   Methods  6-OHDA, APα, γ-aminobutyric acid A receptor(GABAAR) antagonist, voltage-gated L-type Ca2+ channel antagonist were added to the in vitro cultured cell line SH-SY5Y. Immunofluorescence cell chemical staining was used to observe the changes of tyrosine hydroxylase (TH)-positive cells. The changes of the expression of calmodulin (CaM), Ca2+/calmodulin-dependent protein kinase Ⅱ δ3 (CaMKⅡδ3) in the cytoplasm and CaMKⅡδ3, brain derived neurotrophic factor (BDNF) and cyclin-dependent kinases 1(CDK1) in the nucleus of different groups were detected by Western blotting. The interaction between CaMKⅡδ3 and CDK1/BDNF was verified by co-precipitation.   Results  Having treated with APα, the number of TH and 5-Bromo-2-deoxyuridine (BrdU)-positive cells in 6-OHDA-lesioned SH-SY5Y cells increased significantly, but the number of TH/BrdU-double positive cells did not alter significantly. In the cytoplasmic or nucleus fraction of SH-SY5Y cells, the expression of the aforementioned proteins in the APα+6-OHDA group was higher than that in 6-OHDA+DMSO group by Western blotting, in particular, it increased significantly in APα+Bic+6-OHDA group compared with the APα+6-OHDA group. Immunoprecipitation assay showed that there existed an interaction between CaMKⅡδ3 and CDK1 or BDNF.   Conclusion  In the neuroprotective effect of APα on 6-OHDA-lesioned SH-SY5Y cells, GABAAR plays a negative regulation. As a result, APα increases the number of TH-positive neurons by stabilizing the cellular inner environment, in which the Ca2+ -CaM-CaMKⅡδ3 signaling pathway and BDNF, CDK1 plays a key role. References | Related Articles | Metrics

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Amphetamine causing damage of dopamine cells via inhibiting of protein kinase B/glycogen synthase kinase-3β/collapsin response mediator protein-2 signal pathway REN Ya-li GUO Lei PAN San-qiang 2021, 52 (1):  14-20.  doi: 10.16098/j.issn.0529-1356.2021.01.002 Abstract ( )   PDF (6365KB) ( )   ObjectiveTo explore the damage mechanism  of dopamine cells induced by amphetamine (AMPH).   Methods The damage  model of dopaminergic cells in mice was established by intraperitoneal injection of AMPH. The mice were randomly grouped into control, saline, amphetamine treatment for 1 day, 7 days, 14 days and 28 days. Each group contained 10 mice. The model of cell injury was established by use of AMPH in PC12 cells. The dopaminergic fibers of corpus striatum and PC12 cells were observed by the immunohistochemistry and immunofluorescence method , and changes of proteins in the protein kinase B (Akt)/glycogen synthase kinase 3β(GSK-3β)/collapsin response mediator protein 2 (CRMP-2) signal pathway were detected by Western blotting.   Results AMPH caused the damage of dopaminergic fibers in the mouse corpus striatum and PC12 cells. Meanwhile, AMPH inhibited Akt and GSK-3β phosphorylation levels, and increased phosphorylated CRMP-2 level. Nerve growth factor(NGF), an agonist of Akt, or SB216763, an inhibitor of GSK-3β protected PC12 cells against AMPH-induced toxicity through upregulation of Aat and GSK-3β phosphorylation and downregulated of phosphorylation CRMP-2.    Conclusion AMPH causes damage of dopamine cells via inhibition of Akt/GSK-3β/CRMP-2 signal pathway. References | Related Articles | Metrics

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Role and mechanism of stabilizing microtubules of endothelial cells and pericytes in improving the microvasculature dysfunction after spinal cord injury DUAN Yang-yang ZHANG Ya-qun CHAI Yong ZHANG Lu-ping ZHAO Dong-mei YANG Cheng 2021, 52 (1):  21-29.  doi: 10.16098/j.issn.0529-1356.2021.01.003 Abstract ( )   PDF (8469KB) ( )   Objective  To explore the role and mechanism of stabilizing microtubules of endothelial cells and pericytes for ameliorating the dysfunction of microvasculature after spinal cord injury（SCI）.   Methods  The endothelial cells and pericytes from rat brain microvascular tissue (microvessel) were separated and subjected to glucose oxygen deprivation (OGD). The cell viability was detected by CCK-8 and the expression of α-tubulin was detected by immunofluorescence and Western blotting. Rats (n=36) were subjected to dorsal spinal cord transection at T9-10 vertebra. Expression of rat endothelial cell antigen-1（RECA-1）and platelet-derived growth factor receptor β (PDGFRβ)was localized by immunofluorescence. The expression levels of vascular endothelial growth factor A (VEGFA), VEGF receptor 2 (VEGFR2), platelet-derived growth factor-B (PDGFB), PDGFRβ, angiopoietin-1 (Ang-1) and tyrosine kinase with immunoglobulin-like and epidermal growth factor homology domains（Tie-2）were detected by Western blotting.   Results  The cell viability in OGD group, which was time-dependent, was significantly lower than that of the control group, whereas the cell viability in OGD and epothilone B（Epo B）group(OGD-Epo B group) was significantly higher than that of the OGD group. Rupture of cell microtubules was observed after OGD, and the breakage of microtubules was time-dependent. However, the microtubules in OGD-Epo B group were more stable and less destroyed than those of the OGD group. The expression levels of RECA-1 and PDGFRβ in the SCI group were significantly lower than those of the sham group, and the levels in SCI-Epo B group were significantly higher than those of the SCI group at day 2 and day 7 post injury. Moreover, the expression levels of VEGFA, VEGFR2, PDGFB, PDGFRβ, Ang-1 in SCI-Epo B group were significantly higher than the levels of the SCI group.   Conclusion  The endothelial cells and pericytes of microvessels decrease and the microcirculation is dysfunctional after SCI. This corresponds to the microtubule breakage in the cells. The microvessels and pericytes are protected by stabilization treatment of the microtubules, which promotes microcirculation reconstruction and the SCI recovery. References | Related Articles | Metrics

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Changes in expression of estrogen receptor α and β associated with anxiety-like behavior of dams in dams-offspring separation of C57BL/6 J mice HE Feng-qin YU Bing XIANG Quan-li ZHOU Deng-xiang QU Geng-chao 2021, 52 (1):  30-35.  doi: 10.16098/j.issn.0529-1356.2021.01.004 Abstract ( )   PDF (9943KB) ( )   Objective  To investigate the effects of dams-offspring separation on anxiety-like behaviors of dams, and if these anxiety-like behaviors of dams  are associated with estrogen receptorα（ERα）and β（ERβ）in some brain regions.   Methods  Thirty C57BL/6 J female mice were divided into three groups, control group（CG，n=10，non-isolated group）, short-term separation group（SG，n=10, dams were separated from their offspring for 15 minuts per day from the second day to the tenth day after childbirth）and long-term separation group（LG，n=10, dams were separated from their offspring for 3 hours per day from the second day to the tenth day after childbirth）. Anxiety-like behaviors of dams were evaluated in an open-field（OF）and elevated plus-maze test（EPM）. The level of ERα- immunoreactive neurons（ERα-IRs）and ERβ- immunoreactive neurons（ERβ-IRs）in three brain regions including medial preoptic area（mPOA），hypothalamic ventromedial nucleus（VMH）and medial amygdaloid nucleus（MeA）were analyzed.   Results  In OF，compared to CG group and SG group, LG group had significantly less time in center area, crossing number and total distance（P<0.001），there were no significant difference between the CG group and SG group（P>0.05）. In EPM，compared to CG group and SG group，LG group had significantly less percentage of time, distance in open arms and total distance（P<0.001）. Compared to CG group and SG group，LG group had significantly less ERα-IRs and ERβ-IRs in mPOA, VMH，and MeA（P<0.01）.   Conclusion  Dams that are long-termly separated from their offspring may have anxiety-like behavior, and this behavior may be related to the significant reduction of ERα and ERβ in these brain regions. References | Related Articles | Metrics

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Effect of ginkgo biloba extract on behaviors in rat with adolescent sedentariness CHENG Jing YOU Jian-hong CHEN Lin-lin LI Zhen-xi 2021, 52 (1):  36-40.  doi: 10.16098/j.issn.0529-1356.2021.01.005 Abstract ( )   PDF (1069KB) ( )   Objective  To investigate the effect of Ginkgo biloba extract on behaviors in rat with adolescent sedentariness and its possible mechanism.   Methods Twenty-four male SD rats (4-week-old) were randomly divided into normal control (NC), adolescent sedentarines (AS), and Ginkgo biloba extract (GBE) groups. After 4 weeks intervention with GBE, open-field test and elevated plus-maze test were performed to detect the behavioral changes. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD)in brain were determined by colorimetric method. Protein levels of glycogen synthase kinase 3β(GSK-3β) and β-catenin in cerebral white matter were determined by Western blotting.   ResultsIn open-field test, it was shown that the autonomic activity of rats in AS group increased, while the central regional travel time was reduced. Duration and number of residence in the open arm of elevated plus-maze test decreased in AS group. These anxiety-like behaviors were ameliorated by GBE intervention. Compared with the NC group, GSK-3β/β-catenin ratio and the content of MDA was upregulated in AS group while downregulated in GBE group. And activity of SOD were downregulated in AS group while significantly upregulated in GBE group (P<0.05).    Conclusion  Ginkgo biloba extract ameliorate anxiety-like behavior in rat with adolescent sedentariness. Wnt/β-catenin pathway and antioxidant regulation may play a cerebral protective role. References | Related Articles | Metrics

Technology and Methodology

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Comparison of different protocols for protein extraction from formalin-fixed paraffin embedded esophageal squamous cell carcinoma tissues JIAO Ye-lin ZHAO Yun-gang LIU Qi-wei RUAN Hao-jie GAO She-gan QI Yi-jun 2021, 52 (1):  141-145.  doi: 10.16098/j.issn.0529-1356.2021.01.023 Abstract ( )   PDF (6305KB) ( )   Objective  To explore protein extraction efficiency from formaldehyde-fixed paraffin embedded (FFPE) esophageal squamous cell carcinoma (ESCC) tissue samples with different protocols.   Methods  Six different lysis buffers with 100 ℃ or 105 ℃ treatments were used for protein extraction, followed by evaluation of protein quantity and  quality with Bradford, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis,  Western blotting and immunohistochemistry (IHC), using 8 FFPE samples of ESCC.   Results  The optimal method  for protein extraction from FFPE ESCC tissue was Laemmli lysis buffer (Buffer 4) treated with 100 ℃ incubation, evidenced by highest amount of protein recovery. Western blotting and IHC method  measured consistent 14-3-3σ expression in FFPE ESCC tissue samples. Protein precipitated by two volumes of acetonitrite acetonitrile(ACN) (0.1% trifluoroacetic acid) relative to protein amount reduced background staining on SDS-PAGE gels by commassie staining.   Conclusion  Laemmli lysis buffer combined with 100 ℃ incubation has the highest protein extraction efficiency from FFPE ESCC tissue samples for Western blotting measurement of protein biomarkers, and ACN protein precipitation can further eliminate residual cross-linked protein by FFPE. References | Related Articles | Metrics

Review

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Review on the relationship between sphingolipid metabolism and cardiovascular diseases LI Yao LI Jun-lei SUN Yan-rong YANG Qi-yue WANG Wen-juan WANG Ke QIN Li-hua ZHANG Hai-cheng 2021, 52 (1):  146-151.  doi: 10.16098/j.issn.0529-1356.2021.01.024 Abstract ( )   PDF (1123KB) ( )   As bioactive lipids, sphingolipids participate in the signal transduction of many important physiological processes such as growth and apoptosis. Besides, abnormal levels of sphingolipids were detected in a variety of clinical conditions including hypertension and coronary heart disease, suggesting that sphingolipid metabolism is involved in the occurrence and development of cardiovascular diseases. This paper reviewed the relationship between sphingolipid metabolism with four common cardiovascular diseases, coronary heart disease, hypertension, arrhythmia and heart failure, and the mechanisms involved. What’s more, the prospect of sphingolipid pathway as a potential target for the diagnosis and treatment of cardiovascular diseases is put forward. References | Related Articles | Metrics

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A new pathway for mitochondrial quality control: mitochondrial-derived vesicle GUAN Wei-kang Lü Jing YANG Chao-xian 2021, 52 (1):  152-156.  doi: 10.16098/j.issn.0529-1356.2021.01.025 Abstract ( )   PDF (864KB) ( )   Mitochondria are very complex dual membrane organelles in eukaryotic cells. Under physiological conditions, the regeneration and degradation of mitochondria are balanced. When the components of the proteins, lipids and DNA in the organelles are damaged, the steady state of the mitochondria is maintained by means of division, fusion, autophagy and the like, so as to maintain the integrity of the mitochondrial structure and function, which is commonly referred to as a “mitochondrial mass control”. Mitochondrial-derived vesicle (MDV) is a newly discovered pathway of mitochondrial quality control, which plays an important role in the early stage of cell stress and helps maintain the stability of mitochondrial function. In this paper, the discovery of MDV, the transport pathway, the choice of goods and the physiological effects on cells are reviewed. References | Related Articles | Metrics

Medical Education

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How to contribute a manuscript to Acta Anatomica Sinica Jinbo Deng 2021, 52 (1):  157-160.  Abstract ( )   PDF (821KB) ( )   References | Related Articles | Metrics

Histology,Embryology and Developmental Biology

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Decidualization induced by mouse tetraploid embryos ZHANG Bin JIAO Xi-yao LI Hua ZHANG Jing WANG Hao-yang ZHOU Rong-yan LI Xiang-yun 2021, 52 (1):  124-129.  doi: 10.16098/j.issn.0529-1356.2021.01.020 Abstract ( )   PDF (5773KB) ( )   Objective  To investigate the role of inner cell mass (ICM) in decidualization using decidua induced by two-cell embryos or tetraploid embryos through tubal embryo transfer.   Methods  Tetraploid embryo, as the inner cell mass-deficient embryo, were produced by electrofusion of mouse 2-cell embryos. Decidua was induced by 2-cell embryos or tetraploid embryos through tubal embryo transfer. Decidua induced by 2-cell embryos was employed as a control. Morphologic and implantation site of decidua were compared between two-cell embryo-induced decidua and tetraploid embryo-induced decidua. The differentially expressed microRNA (miRNA) was screened by high-throughput sequencing. The target genes of differentially expressed miRNA in two groups were screened for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG).   Results  Tetraploid embryo-induced decidual tissue and 2-cell embryo-induced decidual tissue were very similar at the implantation site, but there were significant differences in decidual morphology. There were 16 miRNAs differentially expressed in decidua of the two groups, of which 11 miRNAs（miR-466f-3p，miR-302 d-3p，miR-466i-5p，miR-465c-5p, miR-302a-5p, miR-7068-3p, miR-741-3p, miR-302a-3p, miR-433-5p, miR-144-5p, miR-878-5p）were up-regulated in tetraploid embryo-induced decidua and 5 miRNAs（miR-690, miR-193b-5p, miR-147-3p, novel_327, miR-363-3p）were down-regulated in tetraploid embryo-induced decidua. Bioinformatics analysis showed that the target genes played function such as protein binding and ion binding, and mainly involved in cyclic guanosine monophosphate-protein kinase G(cGMP-PKG) signaling pathway, estrogen signaling pathway, vascular endothelial growth factor (VEGF) signaling pathway and so on.   Conclusion  ICM plays an important role in decidualization. References | Related Articles | Metrics

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Comparison of clinical outcome between 2 and 3 embryos transfer for elderly women FU Lei ZHOU Wen-hui 2021, 52 (1):  113-117.  doi: 10.16098/j.issn.0529-1356.2021.01.018 Abstract ( )   PDF (3138KB) ( )   Objective  To explore the relationship between clinical outcomes and embryo transfer number, and to provide some proposals for transfer selection of elderly patients.   Methods  Data from 80 fresh transfer cycles with cleavage-stage embryos were analyzed. Cycles were divided into several groups according to transfer number. Clinical pregnancy rate, implantation rate, multiple pregnancy rate and live birth rate were compared.   Results  To women no younger than 38 years old, when available embryo number was larger than two, similar clinical outcomes could be achieved by transferring two and three embryos. This trend was independent of the number of transferred 8-cell embryos.    Conclusion  The number of fresh cleavage-stage embryos transfer should not exceed two in elderly women. References | Related Articles | Metrics

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Transforming growth factor β signaling pathway regulating the function of follicle stimulating hormone in ovarian granulosa cells WANG Yu-tong WEI Xiao-hong GE Ling YANG Yun-feng XU Jian 2021, 52 (1):  118-123.  doi: 10.16098/j.issn.0529-1356.2021.01.019 Abstract ( )   PDF (5581KB) ( )   Objective  To explore the interaction between follicle stimulating hormone (FSH) and transforming growth factor Beta (TGF-β) signaling pathway in rat ovarian granulosa cells.    Methods  The granulosa cells isolated from the follicles of 21 days SD rats. The experiments were divided into three groups: control group, FSH treated group and transforming growth factor beta receptor Ⅱ (TGF-β RⅡ) neutralizated group. Immunocytochemistry (ICC) and Western blotting were then used to locate and detect the expression level of TGF-β RⅡ and proliferating cell nuclear antigen (PCNA). The proliferation index (PI), cell cycle and percentage of apoptotic cells were assessed by flow cytometry (FCM), and the level of estradiol (E2) was determined by ELISA.   Results  FSH increased the expression of PCNA and PI, changed the cell cycle and inhibited apoptosis of GCs, and these actions were reduced significantly when TGF-βsignaling pathway was inhibited(P<0.05); FSH stimulated the secretion of E2, and the increase in E2 levels was not regulated by inhibition of the TGF-β signaling pathway(P>0.05).   Conclusion  The effects of FSH on ovarian granulosa cells are partly affected by the TGF-β signaling pathway. References | Related Articles | Metrics

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MicroRNA144-3p targeting histone deacetylase 2 promoting cardiomyocyte hypertrophy and cardiac function damage TAN Han-xuan PENG Shao-rong HUANG Cai-hua JIANG Hong-feng LIU Juan GONG Fang 2021, 52 (1):  130-134.  doi: 10.16098/j.issn.0529-1356.2021.01.021 Abstract ( )   PDF (3274KB) ( )   Objective  To investigate the role and possible mechanism of microRNA(miR)144-3p in promoting cardiomyocyte hypertrophy.   Methods  Forty-five C57BL/6 mice were divided into control group, myocardial hypertrophy model group (model group), and miR144-3p transfection group (transfection group) according to their transfection method. The cardiac function related indexes of three groups of mice were detected. HE staining was performed on mouse myocardial tissue.The expression of miR144-3p in mouse cardiomyocytes was detected by Real-time PCR. Antinuclear factor (ANF), β-myosin heavy chain (β-MHC), actin α1(Acta1) and histone deacetylase 2 (HDAC2) were detected by Western blotting in three groups.    Results  Compared with the control group, the interventricular septal thickness- diastolic（IVSd）, interventricular septal thickness-systolic（IVSs）, diastolic left ventricular posterior wall thickness（IVPWd）, systolic left ventricular posterior wall thickness（IVPWs）, ejection fraction（EF）, cardiac weight index and left cardiac index of the model group and the transfection group were significantly higher, while systolic left ventricular diameter（LVDs）and diastolic left ventricular diameter（LVDd）were lower (P<0.05), but there was no significant difference between the model group and the transfection group(P>0.05). Compared with the control group, the relative expression of miR144-3p in the model group and the transfection group was significantly higher than that in the model group (P<0.05). Compared with the control group, the expression levels of antinuclear factor, β-myosin heavy chain, Actinα1 and histone deacetylase 2 in the model group and the transfected group were significantly higher (P<0.05).    Conclusion  miR144-3p can aggravate cardiac hypertrophy by up-regulating HDAC2 and is expected to become a new therapeutic target. References | Related Articles | Metrics

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Impact of embryo density on the proportion of good quality cleavage embryos SHI Cheng CHEN Shu-wen WANG Ping LIANG Rong DUAN Sheng-nan CHEN Xi 2021, 52 (1):  135-140.  doi: 10.16098/j.issn.0529-1356.2021.01.022 Abstract ( )   PDF (2574KB) ( )   Objective To investigate the real correlation between embryo density and developmental outcome of cleavage embryos on day 3 by adjusting covariates.   Methods  Data of 1196 embryos from 206 couples undergoing in vitro fertilization and embryo transfer(IVF-ET) treatment were retrospectively analyzed. Embryos were hypoxia-cultivated in a fixed 30 μl microdrop in culture dishes. Embryo quality on day 3 was evaluated and proportion of good quality cleavage embryos on day 3 was used as the endpoint. Maternal age, paternal age, antral follicles, level of anti-mullerian hormone (AMH), type of infertility, controlled ovarian stimulation(COS) protocol, length of stimulation, number of retrieved oocytes, and type of insemination were chosen as covariates.   Results  A total of 1196 embryos were included and analyzed. Three embryo densities were routinely used: 30 μl/embryo ［1 embryo/(30 μl·drop)］, 15 μl/embryo ［2 embryos /(30 μl·drop)］ and 10 μl/embryo ［3 embryos/(30 μl·drop)］. The number of embryos assigned into these three groups were 434, 646 and 116 embryos separately. The average proportion of good quality embryos on day 3 in 10 μl/embryo group were lower than that in both 15 and 30 μl/embryo group (29.31% vs 40.25%，P<0.05；29.31% vs 42.17%，P<0.05), and it was comparable between groups of 15 and 30 μl/embryo (40.25% vs 42.17%，P>0.05). In the regression analysis, without adjusting any covariables, the good quality embryos rate on day 3 of the 10 μl/embryo group was 43% lower than that of the 30 μl/embryo group (0.57, 95% CI 0.32, 1.03), and there was no statistical difference (P>0.05). In the minimum-adjusted model 2 (adjusted the level of AMH and type of insemination), proportion of good quality embryos on day 3 in group of 10 μl/embryo significantly decreased by 51% (0.49, 95%CI 0.27，0.90, P<0.05) compared with that in group of 30 μl/embryo.    ConclusionIn a 30 μl microdrop, compared with individual cul turing, group culturing with the density of 10 μl/embryo did not benefit the development of the cleavage embryos and 30 μl/embryo was the optimal embryo density. References | Related Articles | Metrics

Cancer Biology

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Effects of Angelica Sinensis polysaccharide on proliferation in vitro and transplantation of human leukemia stem cells in vivo DENG Fang-fang GENG Shan JIANG Rong WANG Zi-ling XIAO Han-xian-zhi QI Rong-jia HUANG Cai-hong ZENG Di LI Geng WANG Lu WANG Ya-ping 2021, 52 (1):  41-48.  doi: 10.16098/j.issn.0529-1356.2021.01.006 Abstract ( )   PDF (7071KB) ( )   Objective  To investigate the effect of Angelica Sinensis polysaccharide (ASP) on proliferation, differentiation and transplantation of human leukemia stem cells(LSCs).   Methods  1. Effect of angelica sinensis polysaccharides on proliferation of CD34+CD38-human LSCs in vitro. By using immunomagnetic bead sorting, CD34+CD38-cells isolated from the bone marrow of normal persons and myeloid leukemia patients were divided into normal CD34+CD38-group, CD34+CD38-LSCs group, CD34+CD38-ASP group and CD34+CD38-LSCs ASP group. The latter two groups were added ASP (terminal concentration 40 mg/L) on the basis of conventional culture. The purity of CD34+CD38-cells was detected by flow cytometry. The viability of cells was detected by trypan blue staining. The proliferation of CD34+CD38-cells were detected by cell counting kit 8(CCK-8) and colony forming unit-mixture (CFU-Mix). The expression of aging-related gene was detected by RT-PCR. 2. Effect of Angelica Sinensis polysaccharide on mouse model of LSCs transplantation. After to establish CD34+CD38-LSCs transplantation mice model, the mice were randomly divided into ASP transplantation model group (intraperitoneal injection with ASP,  200 mg/kg, Qd×14 days) and transplantation model group (intraperitoneal injection with the same volume of saline at the same time). On the 2nd day after drug injection was finished, leukocytes count and classification and cells morphology were observed. By using immunofluorescence cytochemistry, CD34+CD38-LSCs in bone marrow of recipient mice was detected. The percentage of senescence assosiated β-galactosidase(SA-β-Gal) positive staining cells in bone marrow mononuclear cells (BMMCs) was detected. Cell cycle distribution of BMMCs was detected by flow cytometry. The colony formation ability of BMMCs was detected by CFU-Mix.  ResultsThe purity of CD34+CD38- cells was (91.14±1.02)% and the viability of cells was (95.42±3.52)%. The proliferation and CFU-Mix formation ability of CD34+CD38-LSCs group were significantly higher than that of normal CD34+CD38-group. The proliferation and CFU-Mix formation ability of CD34+CD38- LSCs ASP group were obviously lower than that of CD34+CD38-LSCs group. The expressions of p16INK4a, p19Arf, p53, p21Cip1/Waf1 genes in CD34+CD38-LSCs ASP group increased. Human LSCs existed in the bone marrow of recipient mice transplanted LSCs. The count of leukocytes in the transplantation model group increased, the proportion of neutrophils increased and lymphocytes decreased. After injection of ASP, the count of leukocytes and the proportion of neutrophils were markedly reduced, meanwhile, the proportion of lymphocytes increased. The number of BMMCs in G0/G1 phase increased and in S phase decreased. The percentage of SA-β-Gal positive staining cells increased significantly; the number of CFU-Mix decreased.  Conclusion  ASP inhibits the proliferation of CD34+CD38-LSCs in vivo or in vitro. It is suggested that the mechanism is closely related to the expression of genes related to the regulation of cellular senescence. References | Related Articles | Metrics

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Inhibition of T lymphocytes secreting EphrinAl-Caspase-3 on proliferation of tumor tissue in nude mice with breast cancer SHI Ya-qi TANG Xi-min LI Zhuang ZHANG Li-mei LU Xue-jing ZHOU Ling-zhi XU Mang HUANG Yu LI Yan-jiao ZHANG Ben-si 2021, 52 (1):  49-54.  doi: 10.16098/j.issn.0529-1356.2021.01.007 Abstract ( )   PDF (3922KB) ( )   Objective  To study the inhibitory effect of T lymphocytes secreting EphrinAl-Caspase-3 in vivo and on the growth of cancer cells in nude mice with breast cancer.   Methods  Nude mice（n=35）were inoculated with breast cancer cells to construct a nude mouse model of breast cancer. When the tumor volume reached 0.1 cm3, 30 nude mice with average size tumor tissue were randomly divided into PBS group,uninfected adenovirus group, T lymphocyte infected with Ad-EphrinA1-Caspase-3 group, and intratumoral transplantation. Tumor size was measured every day 2 to 3. Three groups of tumor-bearing nude mice were selected. After the above-mentioned cell transplantation, the subcutaneous tumor tissue homogenate was obtained every day 2 to 3, and the content of EphrinA1-Caspase-3 was detected by ELISA. At the end of the experiment, the animals in each group were sacrificed by cervical dissection and sliced. The presence of T lymphocytes expressing green fluorescent protein was observed under a fluorescence microscope, and Caspase-3 and Ki-67 were detected by immunofluorescence.    Results  After one week of inoculation of breast cancer cells into nude mice, the presence of subcutaneous tumors could be touched by hand, which proved that the tumor-bearing animals of breast cancer cells were successfully modeled. On the 8th day after inoculation, the tumor volume of the nude mice in each group became larger, and the difference between the treatment group and the PBS group/T lymphocyte group was extremely significant (P<0.05). Although the tumor volume of the T lymphocyte transplantation group was slower than that of the PBS control group, there was no statistically significant difference between the two. The expression of EphrinA1-Caspase-3 was detected in the EphrinA1-Caspase-3 treatment group on the 2nd day, reached the peak on the 8th day, and then the secretion decreased gradually. No expression of EphrinA1-Caspase-3 was detected in the PBS control group and the T lymphocyte group. The presence of dispersed green fluorescent protein-labeled EphrinAl-Caspase-3-T lymphocytes was observed in the tumor tissues of the treatment group, while the presence of green fluorescent protein was not detected in the PBS group and the T lymphocyte groups. In the infected cells of the treatment group, the proportion of Caspase-3 positive cells was up-regulated, and the proportion of Ki-67 positive cells was down-regulated. No expression of EphrinAl-Caspase-3 was detected in the PBS group and the T lymphocyte group.   ConclusionEphrinAl-Caspase-3 can significantly inhibit the growth of breast cancer cells, thereby exerting an anti-tumor effect. References | Related Articles | Metrics

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Construction of Rho GDIα-sgRNAs plasmids by clustered regularly interspaced short palindromic repeats/associated protein 9 and the effect on migration of Hepa 1-6 cell line in mouse QU Ming-juan LI Yan-min XIE Jing QIN Miao-miao WANG Jing ZHOU Ju-hua 2021, 52 (1):  55-59.  doi: 10.16098/j.issn.0529-1356.2021.01.008 Abstract ( )   PDF (5112KB) ( )   Objective  To construct the recombinant plasmids of knocking down Rho guanine dissociation inhibitor α (GDIα) gene by using clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) technique, and investigate the effect of Rho GDIα interference on the migration of Hepa 1-6 cells of mouse in order to provide the method  of prevention and treatment of liver cancer.   Methods  To construct and identify the PX458-Rho GDIα-single guide(sg)RNAs by using CRISPR/Cas9 technique. And the Hepa 1-6 cells were transfected by liposomes with PX458-Rho GDIα-sgRNAs for 48 hours respectively, and cells treated with PX458 plasmids were used as control. The migration ability of Hepa 1-6 was checked by wound healing assay and Transwell assay, respectively.  Results  The expression of Rho GDIα was depressed in group of PX458-Rho GDIα-sgRNA1 transfection which was detected by using RT-PCR. The migration distance of Hepa 1-6 in PX458-Rho GDIα-sgRNA1 transfection group was significantly promoted comparing with the control group which was transfected with PX458 only, and the cell number of PX458-Rho GDIα-sgRNA1 group was more than that in control group by using transwell assay, indicating concluded that knocking down of Rho GDIα promoted the migration ability of Hepa1-6 cells.   Conclusion  The result  is explicit that in vivo, Rho GDIα may inhibit the migration of Hepa1-6 partially. Overexpression of Rho GDIα  might be used as an important method  to prevent the metastasize of carcinoma. References | Related Articles | Metrics

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Effects of Rab1A on proliferation and apoptosis of multiple myeloma cell line 8226 WU Han WANG Xiu-hong WU Ting-ting YANG Su 2021, 52 (1):  60-66.  doi: 10.16098/j.issn.0529-1356.2021.01.009 Abstract ( )   PDF (3047KB) ( )   Objective  To investigate the effect of Rab1A on proliferation and apoptosis of multiple myeloma(MM) cell line 8226.   Methods  The siRNA interference was used to knockdown Rab1A gene. The multiple myeloma cell line 8226 was divided into blank control group, negative control group and Rab1A siRNA group. In the blank control group, the multiple myeloma cells were not treated. Multiple myeloma cells 8226 in the negative control group were transfected with negative control siRNA. The Rab1A siRNA group was transfected with Rabl A-targeted siRNA. The effect of Rab1A on multiple myeloma cell 8226 proliferation was analyzed by  colony forming test and cell counting kit-8 (CCK-8) assay. The apoptosis of multiple myeloma cell 8226 was detected by flow cytometry. Western blotting and Real-time PCR were used to observe the effect of Rab1A siRNA on the expression of c-Myc, cyclin D1, Bcl-2 and Bax.   Results  The expressions of Rab1A mRNA and Rab1A protein in the Rab1A siRNA group were significantly down-regulated compared with those in the negative control group. The result  of colony formation and CCK-8 assay showed that Rab1A siRNA inhibit the proliferation of multiple myeloma cells 8226. The early and late apoptosis ratio of multiple myeloma cell 8226 in Rab1A siRNA group increased significantly compared with the negative control group (P<0.05). The expression of cyclin D1 and Bcl-2 in the Rab1A siRNA group were significantly down-regulated compared with the negative control group (P<0.05), and the expression of c-Myc and Bax were significantly up-regulated compared with the negative control group (P<0.05).   ConclusionRab1A may promote the proliferation of multiple myeloma cells 8226 by regulating the expression of c-Myc, cyclin D1, Bcl-2 and Bax, while RablA siRNA can effectively inhibit the expression of RablA in rpmi-8226 cells, thereby inhibiting its proliferation and promoting apoptosis. References | Related Articles | Metrics

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Effect of melittin on programmed necrosis of human hepatocellular carcinoma cell line SMMC-7721 LI Ya-wei ZHANG Wei LI Yan HOU Jian-cheng ZHANG Hong ZHU Wen-he 2021, 52 (1):  67-72.  doi: 10.16098/j.issn.0529-1356.2021.01.010 Abstract ( )   PDF (6413KB) ( )   Objective  To investigate the lethal effect of melittin on human hepatocellular carcinoma cell line SMMC-7721 and its possible mechanism.   Methods  MTT assay was used to investigate the killing effect of different concentrations melittin on human hepatocellular carcinoma cells SMMC-7721. Meanwhile, the inhibitory effect of specific programmed necrosis inhibitors necrostain-1(Nec-1) on melittin killing SMMC-7721 cells was detected. Programmed cell necrosis observed by Hoechst 33342 and PI double staining. The necrotosis rate was detected by flow cytometry. Ultrastructural changes of cell was detected by transmission electron microscopy. The expression of receptor interacting protein 1(RIP1) was detected by Western blotting.   Results  Compared with the control group, the proliferation activity of SMMC-7721 cells was significantly decreased after treatment with different concentrations of melittin for 24 hours (P<0.05). Cells stained in dark blue and red. Cell membrane integrity was destroyed, organelle swelling, organelle membrane was destroy, that demonstrates cell was necrosis. Westen blotting result  showed an increased proportion of RIP1 expression in SMMC-7721 cells. Compared with the melittin group, cell proliferation activity was significantly increased, cell necrotosis rate was decreased, and intracellular RIP1 expression was down-regulated in the Nec-1 pretreatment group.  Conclusion  Melittin induces cell death in SMMC-7721 cells through the RIP1-mediated programmed necrosis pathway. References | Related Articles | Metrics

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MicroRNA-513c-5p expression in cervical cancer and targeting histone deacetylase 1 regulating cervical cancer  cell migration and invasion WANG Li-na LIU Yong-juan ZHANG Ying LIU Rong-xia LIANG Shan SONG Chun-hong CUI Na 2021, 52 (1):  73-77.  doi: 10.16098/j.issn.0529-1356.2021.01.011 Abstract ( )   PDF (4562KB) ( )   Objective  To investigate the expression  of microRNA (miR)-513c-5p in cervical cancer and the mechanism  of targeting histone deacetylase 1 (HDAC1) regulating cervical cancer cell migration and invasion.   Methods  Clinically collected 86 patients with cervical cancer. The levels of miR-513c-5p in tumor tissues and adjacent tissues were detected by Real-time PCR. The relationship between miR-513c-5p and pathological characteristics of cervical cancer was analyzed. It was verified that miR-513c-5p targets HDAC1 by a dual luciferase report. Cervical cancer HeLa cells were divided into four groups: control group, mimic group, mimic+HDAC1 group and HDAC1 group. MiR-513c-5p and(or) HDAC1 were overexpressed by plasmid transfection technology. Real-time PCR and Western blotting were used to detect the expression level of RNA or protein, respectively. The cell growth, migration, and invasion capabilities of each group were measured by CCK-8 method , cell scratch test, and Transwell test.   Results  The level of miR-513c-5p in cervical cancer tissues was significantly lower than that in adjacent tissues. Low levels of miR-513c-5p were associated with higher local invasion, lymphatic metastasis, and distal metastasis (P<0.05). MiR-513-5p targeted HDAC1 expression. Overexpression of miR-513c-5p inhibited significantly the growth, migration and invasion of cervical cancer cells (P<0.05). Overexpression of HDAC1 promoted growth, migration and invasion (P<0.05), and reversed the inhibitory effect of miR-513c-5p (P<0.05).   Conclusion  Low levels of miR-513c-5p might be related to cervical cancer metastasis, and miR-513c-5p could inhibit the growth, migration and invasion of cervical cancer HeLa cells by targeted inhibition of HDAC1 protein expression. References | Related Articles | Metrics

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Expression and mechanism of microRNA-27a in cervical cancer ZHANG Hong-ping LI Feng NIU Zhan-jie 2021, 52 (1):  78-83.  doi: 10.16098/j.issn.0529-1356.2021.01.012 Abstract ( )   PDF (3256KB) ( )   Objective  To investigate the effects of microRNA (miR)-27a on proliferation, apoptosis and invasion of cervical cancer cells by targeting F-box and WD repeat domain containing protein 7( FBXW7) expression.   Methods  Thirty cases of cervical cancer and paracancerous tissues and 30 cases of normal tissues were used in the experiment. The expression of miR-27a in cervical cancer, paracancerous tissue, normal cervical tissue, cervical cancer cells (SiHa, Caski, HeLa, HCC94) and cervical squamous epithelial immortalized cell H8 were detected by Real-time PCR. MiR-27a inhibitor and its negative control were transfected into SiHa cells by liposome transfection. CCK-8 assay, flow cytometry and Transwell assay were used to detect the effects of miR-27a on the proliferation, cell cycle, apoptotic rate and invasive ability of SiHa cells.Bioinformatics was used to predict the targeting gene of miR-27a. Double luciferase reporter gene assay combined with Western blotting was used to verify the targeting regulation of miR-27a on FBXW7.   Results  Compared with the normal cervical tissues and the adjacent tissues, the expression of miR-27a was higher in cervical cancer tissues (P<0.05); Compared with the cervical squamous epithelial immortalized cells H8, the expression of miR-27a in cervical cancer cells SiHa, Caski, HeLa and HCC94 was higher (P<0.05). Inhibiting the expression of miR-27a in SiHa cells could significantly reduce the proliferation activity of cells (P<0.05), increase the proportion of G0/G1 cells (P<0.05), decrease the proportion of G2/M cells (P<0.05), increase the apoptosis rate (P<0.05), and inhibit the invasive ability of cells (P<0.05). Bioinformatics predicted that FBXW7 might be a target regulatory gene of miR-27a. Double luciferase reporter gene assay showed that miR-27a could specifically bind to the 3’UTR region of FBXW7 (P<0.05), and negatively regulate the expression of FBXW7 protein (P<0.05).   Conclusion  MiR-27a plays an oncogene role in the occurrence and development of cervical cancer. Inhibiting the expression of miR-27a can significantly inhibit the malignant biological behavior of cervical cancer cells, and its mechanism may be related to targeted regulation of FBXW7 expression. References | Related Articles | Metrics

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Three-dimensional digital measurement of occipital condyle and foramen magnum in Inner Mongolia WU Chao WANG Yi-dan GUAN Huan-huan GAO Ming-jie ZHANG Yun-feng WANG Hai-yan HE Yu-jie GAO Shang LI Zhi-jun LI Xiao-he 2021, 52 (1):  84-90.  doi: 10.16098/j.issn.0529-1356.2021.01.013 Abstract ( )   PDF (3938KB) ( )   Objective  To measure the anatomical structure of the occipital condyle (OC) and the occipital foramen (FM) by three-dimensional reconstruction, and to analyze the morphological characteristics and relative positional relationship of the occipital condyle and occipital foramen, in order to provide anatomical parameters for the imaging diagnosis of the craniocervical junction and the choice of surgical approach.    Methods  Sixty normal subjects were selected with CT scans of the skull and upper cervical spine, including 30 males and 30 females, aged 20-65 (48.18±16.17) years old. The data were imported into the Syngo.via VB10B software, and the skull was reconstructed in three dimensions. To observe the shape of the occipital condyle and occipital foramen, and to measure the occipital condyle length, width, height, condyle inclination angle(CIA), longitudinal diameter, transverse diameter, area of the occipital foramen, the maximum distance between the cranial eyebrow and the posterior cranial point (SML), the crimson eyebrow on the SML line, the distance from the interpoint to the posterior margin of the occipital condyle (GOCP), the vertical distance between the anterior edge of the occipital foramen to the posterior margin of the occipital condyle (AOCP), and the distance from the medial margin of the left and right occipital condyles to the Y axis (OC-M), left and right occipital condyle posterior margin to X ax  s distance (OC-P); occipital condyle classification index (OCI), occipital condyle relative index of head (SOCI), midpoint on the SML straight line to the occipital condyle Marginal connection distance (COCP,COCP=GOCP-SML/2), and determine the type of relative positional relationship between left and right occipital condyles.   Results  The differences in anatomical length, width and height of the occipital condyle were statistically significant (P<0.05), and men were larger than women; the occipital foramen area, longitudinal diameter of the occipital foramen, SML, GOCP, AOCP had statistical differences (P<0.05). The lateral differences of occipital condyle inclination were statistically significant (P<0.05), and the left side was greater than the right side. The differences in OC-M and OC-P sides were statistically significant (P<0.05). The former was larger on the right than on the left; the latter was larger on the left than on the right. The longitudinal diameter of the occipital foramen was positively correlated with the area of the occipital foramen and AOCP; OCI classification result  were as follows: type Ⅰ (OCI<0.45) had 8 cases (13.33%), type Ⅱ (0.45≤OCI<0.50) had 47 cases (78.33%), type Ⅲ (OCI≥0.50) had 5 cases (8.33%). SOCI classification result  were as follows: type Ⅰ (SOCI<0.60) had 2 cases (3.33%), type Ⅱ (0.60≤OCI<0.75) had 54 cases (90.00%), type Ⅲ (SOCI≥0.75) had 4 cases (6.67%).   Conclusion  The anatomical parameters of the occipital condyle in Inner Mongolia can be implanted with occipital condylar screws. The position of the occipital condyle relative to the foramen magnum and the skull is highly variable. References | Related Articles | Metrics

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Susceptibility weighted imaging of the veins around foramen of monro YAO Xiao-xiao LI Chang-sheng CHEN Dai-xi GUO Yu MIAO Hui-zhong YANG Xin-dong CHEN Zheng-zhen CHEN Cheng-chun 2021, 52 (1):  91-97.  doi: 10.16098/j.issn.0529-1356.2021.01.014 Abstract ( )   PDF (11238KB) ( )   Objective  To image the veins around the foramen of monro (FM), to build the 3D model of them, to construct venous network in this area and to explore the spatial positional correlation between FM and veins around it.   Methods  Totally 60 healthy subjects were selected to get the original images on 3.0 T MR and procesed the original images by minimum intensity projections (mIP) and Materialise’s interactive medical image control system (Mimics), built the 3D model of the veins around FM, observed and analyzed the morphology of FM and the veins around it on original and processed images.    Results  The displaying rate of FM was 65% (78 sides), the displaying rate of internal cerebellar veins (ICV) was 100% (120 sides), the diameter was (2.13±0.30)mm. The displaying rate of anterior septal vein (ASV) was 100% (120 sides), the diameter was(0.69±0.19)mm. The displaying rate of superior thalamostriate vein (STV) was 98.3% (118 sides), the diameter was (1.47±0.38)mm. The displaying rate of superior choroidal vein (SCV) was 82.5% (99 sides), the diameter was(0.40±0.18)mm. According to the relationship between the converging point of the tributaries of ICV and the location of FM, FMs were classified into 5 types:ⅠA, 24.2% (29 sides), ASV converged into ICV at the venous angle and closed to the posterior edge of FM; ⅠB, 13.3% (16 sides), ASV converged into ICV away from the venous angle and the posterior edge of FM; ⅡA, 45% (54 sides), ASV converged into ICV at the false venous angle and closed to the posterior edge of FM; ⅡB, 15.8% (19 sides), ASV converged into ICV away form the false venous angle  and the posterior edge of FM. Ⅲ, 1.7% (2 sides), STV was absent.  Conclusion FM and the veins around it are visible on the susceptibility weighted imaging(SWI). It can be constructed by Mimics that the 3D model of ICV, its tributaries, FM and the converging points of the major veins. The classification of FMs is meaningful to the option of surgical approaches through FM. References | Related Articles | Metrics

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Digital measurement and clinical significance of imaging parameters of occipital-C2 angle and posterior occipitocervical angle QU Xing-yue BU Jia-qi SHI Jun DUAN Jin-dong ZHOU Wen-hui LI Zhi-jun WANG Xing ZHANG Shao-jie 2021, 52 (1):  98-102.  doi: 10.16098/j.issn.0529-1356.2021.01.015 Abstract ( )   PDF (2110KB) ( )   Objective  To explore the differences and correlation of imaging parameters of occipital-C2 angle (OC2A) and posterior occipitocervical angle (POCA) between sex and age, so as to provide theoretical reference for fixing the position of head and neck in occipitocervical fusion.   Methods  The imaging data of 473 cases (339 males and 134 females) were collected and divided into 2 groups according to sex. Each group was subdivided into 6 groups according to age: ≤29 year-old, 30-39 year-old, 40-49 year-old, 50-59 year-old, 60-69 year-old and ≥70 year-old. OC2A and POCA were measured in Mimics software, and their differences with sex and age were statistically analyzed.   Results  There was no significant difference in OC2A and POCA between sexes (P>0.05). In the OC2A male group, except for the male group aged 30-39 year-old and ≤29 year-old, there were significant differences among the other groups (P<0.05), but there was no significant difference among all age groups in the female group (P>0.05); There were significant differences in POCA between the male group of 30-39 year-old and ≤29 year-old (P<0.05), but between the group of 40-49 year-old and each group (P<0.05). In the female group, there was significant difference between the group of ≤29 year-old and all age groups(P<0.05), but there was no significant difference among the other groups (P>0.05). Pearson correlation analysis showed that there was a positive correlation between OC2A and POCA (r=0.038, P>0.05), that is, there was no correlation between them.   ConclusionThere is no difference in OC2A and POCA values between sexes; there are differences in OC2A and POCA values in males among different age groups, suggesting that clinical attention should be paid to the age differences in males, while there is no difference in OC2A values in females, but POCA is different in different age groups. The changes of OC2A and POCA values in different age groups and sex provide a parameter basis for fixing the anatomical reduction angle of head and neck in occipitocervical fusion. References | Related Articles | Metrics

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Measurement of spino-pelvic anatomical parameters and finite element analysis of lower lumbar vertebra in lumbarization patients BI Dian-hai LUO Shao-lin PEI Na ZENG Huan-gao 2021, 52 (1):  103-107.  doi: 10.16098/j.issn.0529-1356.2021.01.016 Abstract ( )   PDF (10647KB) ( )   Objective  To measure the sagittal anatomical parameters of the spine and pelvis based on EOS imaging system, and to evaluate the stress of the lower lumbar spine by finite element analysis(FEA).   Methods  A total of 44 subjects examined by EOS imaging system were included, including 11 sacral lumbar vertebra patients and 33 normal subjects. The sagittal plane parameters of lumbar lordosis (LL), pelvic incidence (PI), pelvic tilt (PT) and sacral slope (SS) were measured and compared in both groups. Pearson test was used to analyze the correlation between PI and LL in the two groups. At the same time, the finite element model of the lower lumbar vertebra was established. The stress condition of the lumbar spine model during forward bending, backward extension and left and right bending was evaluated by FEA method.   Results  The differences of PI, PT, SS and LL between the two groups were statistically significant (P<0.05). The correlation study found that there was a positive correlation between the two groups of subjects’ PI and LL, including the lumbarization group (r=0.69, P<0.05) and the normal group (r=0.52, P<0.05). Under the conditions of forward bending, backward stretching, left bending and right bending, the bending moment of the model was 2 Nmm, and the stress concentration gradually decreased from bottom to top. The maximum stress concentration point was located at the lower articular process.   Conclusion  The physiological curvature and stress distribution of the lumbar spine in lumbarization population were different than normal, especially the stress concentration of the transitional intervertebral disc and articular process joint was obvious, and early degeneration of the spine was easy to occur. References | Related Articles | Metrics

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Laparoscopic precise positioning for radical prostatectomy or bladder cancer reducing the risk of delayed recovery LI Ying-yun ZHOU Zhi-jun 2021, 52 (1):  108-112.  doi: 10.16098/j.issn.0529-1356.2021.01.017 Abstract ( )   PDF (858KB) ( )   Objective  To analyze the risk factors of delayed recovery after laparoscopic prostate or bladder cancer radical surgery, and to provide information for early clinical prevention.   Methods  Three hundred and twenty-two cases of patients who underwent laparoscopic radical prostatectomy or bladder cancer surgery from September 2016 to January 2019 were enrolled in this study. The clinical data and surgical data of the patients were collected, the incidence of postoperative recovery delay was counted, and the risk factors of delayed recovery were analyzed by Logistic regression analysis.   Results   Six-noe cases of delayed recovery of laparoscopic prostate or bladder cancer were detected, the incidence rate was 18.7% (61/327); Univariate analysis found that  delayed laparoscopic recovery of prostate or bladder cancer after radical surgery and age, combined with coronary heart disease, diabetes, respiratory disease, anemia, smoking, alcohol consumption, American Society of Anesthesiologists(ASA) classification, anesthesia time, intraoperative infusion，location clarity of anatomical landmarks were related. There was a correlation between the total amount, combined epidural anesthesia and intraoperative blood transfusion (P<0.05). Multivariate logistic regression analysis found that the age, diabetes, respiratory disease, anemia, smoking, alcohol consumption, location clarity of anatomical landmarks and intraoperative total infusion were independent risk factors for delayed recovery after laparoscopic prostate cancer or radical surgery for bladder cancer (P<0.05).   Conclusion  There is correlation between delayed laparoscopic recovery of prostate cancer or bladder cancer after radical operation and age, diabetes, respiratory disease, anemia, smoking, alcohol consumption, location clarity of anatomical landmarks and intraoperative total infusion. Accurate anatomical location can reduce the risk of postoperative recovery delay. It is conducive to the recovery of the patients after operation, and the corresponding hospitalization time of the patients is greatly shortened. References | Related Articles | Metrics