解剖学报

解剖学报 ›› 2019, Vol. 50 ›› Issue (1): 8-12.doi: 10.16098/j.issn.0529-1356.2019.01.002

• 神经生物学 • 上一篇    下一篇

应用转录组和外显子组测序分析不同年龄组的胶质母细胞瘤中基因表达与突变的差异

王志刚1 刘伟2*   

  1. 1. 中国医学科学院基础医学研究所医学生物工程学系;  2. 人体解剖学与组织学胚胎学系，北京 100005
  • 收稿日期:2018-03-22 修回日期:2018-04-12 出版日期:2019-02-06 发布日期:2019-04-18
  • 通讯作者: 刘伟 E-mail:2436914829@qq.com

Analysis of the difference in gene expression and mutation of glioblastoma in different age groups by using the transcriptional and exome sequencing

WANG Zhi-gang1 LIU Wei 2*   

  1. 1. Department of Medical Engineering; 2. Department of Anatomy and Histology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing 100005, China
  • Received:2018-03-22 Revised:2018-04-12 Online:2019-02-06 Published:2019-04-18
  • Contact: LIU Wei E-mail:2436914829@qq.com

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摘要：

目的 探讨通过转录组和外显子组测序分析不同年龄组的胶质母细胞瘤的差异。 方法 多形性胶质母细胞瘤（GBM）数据下载自TCGA项目。将样本分为2组，不高于60岁的样本为中低年龄组，共78例；高于60岁的样本为高年龄组，共76例。使用R语言DESeq2软件包对RNA-seq原始基因水平的reads数目数据进行差异表达分析。使用Cytoscape插件ClueGO进行不同年龄组别差异表达基因功能富集分析。 结果 GBM致病基因在两组之间大部分基因表达趋势一致，但存在差异基因。在中低龄组中差异基因主要和神经前体细胞增殖相关，而高年龄组偏重于代谢方面。在两组中高突变的基因有很多重叠。样本突变率不同的基因主要为中低年龄组特有，且具有高样本突变率的基因。 结论 中低年龄组和高龄组GBM患者在基因表达和基因突变上存在明显差异。

关键词: 转录组测序, 年龄, 胶质母细胞瘤, 全外显子组测序, 人

Abstract:

Objective To explore the gene expression difference of glioblastoma in different age groups through the sequencing of the transcriptome and exome. Methods Glioblastoma multiforme(GBM) data was downloaded from the Cancer Genome Atlas(TCGA), and the samples were divided into two groups. There were 78 cases in the middle and low age group not more than 60 years old, compared with the high age group of 60 years old, with a total of 76 cases. DESeq2 package was used to analyze differentially expressed genes based on gene level reads numbers. Cytoscape plug-in ClueGO was used to analyze gene function enrichment in different age groups. Results Most of the gene expression trends in the two groups were consistent between the two groups, but there were some different genes. In the middle and low age group, the differential gene was mainly related to the proliferation of neural precursor cells, while the high age group was metabolic. In the two groups, the high mutation genes were basically consistent, but the mutation rates were different. The mutation differentially genes were concentrated in the middle and low age groups. Conclusion There is a significant difference in gene expression and gene mutation between the middle and low age group and the elderly GBM group.

Key words: RNA-sequencing, Age;Glioblastoma, Exome-sequencing, Human