关键词: 三氮唑席夫碱衍生物, 肝癌细胞, 多倍体巨细胞, 微管, 免疫荧光, 人

Abstract: Objective To analyze the features of polyploid giant cells that come from SMMC-7721 cells after being induced by triazole Schiff base derivative(LH-37) EM>in vitro/EM>. Methods The SMMC-7721 cells（1×10SUP>4/SUP>/ml） were cultivated in medium containing of 1×10SUP>-5/SUP> mol/L LH-37 for 72 hours, and their DNA content was analyzed by flow cytometry. The Co-staining with Hoechst 33258 and propidium iodide were used to observe cell death. Behavior of the microtubule(MTs) and laminB configuration apparatus in cells were examined by immunofluorescence, counterstained with Hoest33258 staining，and the ultrastructure of giant tumor cells was observed by JEM 100CX-II electron microscopes.Results Increased the proportion of mono-nucleated or multi-nucleated giant cells (MOGC and MNGC) after SMMC-7721 cells were cultured in the presence of LH-37 at 72 hours. The proportion of the giant cells in MOGC and MNGC was 12.32%and 0.92% respectively. Arrangement and radial segregation of subnuclei, subnuclei were united through MTs which radically emerged and embraced them from a single microtubule organizing centre (MTOC),thick microtubule tracks reached individual nucleoli; sub-nuclei of this radial arrangement was encircled by the intact lamin; and most of the MNGCs died by apoptotic crisis. MTs of MOGC uniformly spread throughout the cytoplasm from the nuclear envelope and usually accumulated in the perinuclear region. Abnormal mutipolar mitosis, and spontaneous chromosome condensation were observed. In addition electron micrographs demonstrated features of autophagy during apoptosis of some MOGCs. Conclusion The data presented indicate th

Key words: Triazole Schiff base derivative, Human hepatocarcinoma cell, Polyploid giant cells, Microtubule, Immunofluorescence, Human