解剖学报

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1. 热应激猪睾丸葡萄糖转运蛋白1和葡萄糖转运蛋白2的表达与定位

席华明范小瑞张禛梁亚俊贺俊平

解剖学报 2017, 48 (4): 445-451. DOI: 10.16098/j.issn.0529-1356.2017.04.013

摘要（3059）

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目的探讨正常和热应激条件下，葡萄糖转运蛋白1（GLUT1）和葡萄糖转运蛋白2（GLUT2）在成年猪睾丸的表达和定位。方法性成熟长白公猪9头，随机分为3组。局部阴囊热刺激组（n=3），用自制电热毯置阴囊42 ℃加热1 h；环境热应激组（n=3），每天置于37～40 ℃猪舍环境3 h，连续7 d，每天于热处理结束后，将实验猪驱赶回21～25 ℃猪舍环境；对照组（n=3），饲养在21～25 ℃猪舍环境。局部热刺激6 h后和环境热应激处理结束24 h后，手术摘除双侧睾丸。用Real-time PCR、Western blotting和免疫组织化学技术检测猪睾丸组织内GLUT1和GLUT2的表达。结果 Real-time PCR和Western blotting结果显示，与对照组相比，环境热应激组GLUT1蛋白和mRNA的表达差异不显著，局部阴囊热刺激组GLUT1蛋白和mRNA表达显著升高；环境热应激组和局部阴囊热刺激组，GLUT2蛋白和mRNA表达均显著升高。免疫组织化学结果发现，热处理前后，GLUT1蛋白在曲精小管内定位于精母细胞和圆形精子细胞；环境热应激组GLUT1蛋白染色与对照组相比，无明显差异，局部阴囊热刺激后，GLUT1染色变深，表达升高。热处理前后，GLUT2蛋白在曲精小管定位于生精细胞和支持细胞，环境热应激和局部阴囊热刺激导致GLUT2染色变深，表达升高。结论葡萄糖转运蛋白GLUT1和GLUT2表达于猪睾丸曲精小管，环境高温和阴囊局部热刺激导致GLUT1和GLUT2在猪睾丸的表达水平改变，提示这两种葡萄糖转运蛋白在猪精子发生过程中发挥重要作用。

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2. 白藜芦醇对NIH3T3细胞Shh信号通路的影响

郭霜廖鸿雁刘杰唐凡人杨琴

解剖学报 2018, 49 (2): 179-184. DOI: 10.16098/j.issn.0529-1356.2018.02.007

摘要（2937）

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目的探讨白藜芦醇对NIH3T3细胞Shh信号通路的影响。方法实验分为对照组和白藜芦醇组。白藜芦醇处理NIH3T3细胞24 h。CCK-8法测定细胞活力，免疫荧光法检测初级纤毛蛋白（Ac-tu）、Smo和Gli-1的表达，Western blotting法检测Shh、Ptc、Smo、Gli-1蛋白的表达。结果 1. 0.5和1 μmol/L白藜芦醇组（0.679±0.047, 0.774±0.054）细胞活力分别较对照组（0.585±0.039）明显增强（P＜0.05），其中以1 μmol/L白藜芦醇组最强。之后随白藜芦醇浓度（10、20、40、80 μmol/L）的增高，细胞活力逐渐降低（0.428±0.043, 0.395±0.031, 0.373±0.017, 0.361±0.016），且均较对照组明显减弱（P＜0.05），而0.1 μmol/L（0.602±0.065）和5 μmol/L组（0.556±0.041）细胞活力与对照组比较差异无显著性（P>0.05）。2. NIH3T3细胞表达Ac-tu、Shh、Ptc-1、Smo、Gli-1从蛋白，有1根初级纤毛，Smo和Gli-1蛋白位于细胞质。白藜芦醇处理24h时，Gli-1从细胞质进入细胞核，Smo从细胞质进入初级纤毛，且Shh（0.756±0.659比0.441±0.769，P＜0.05）、Ptc-1（0.655±0.347比0.351±0.026，P＜0.05）、Smo（0.779±0.064比0.451±0.035，P＜0.05）和Gli-1（0.856±0.044比0.560±0.058，P＜0.05）蛋白表达较对照组高。结论白藜芦醇可能通过激活Shh信号通路增强NIH3T3细胞的活力。

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3. 索拉非尼对大鼠佐剂性关节炎的抑制作用

刘菲龚永芳王真珍张晓明邓雪飞焦轶黄学应

解剖学报 2017, 48 (2): 187-192. DOI: 10.16098/j.issn.0529-1356.2017.02.012

摘要（2830）

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目的探讨索拉非尼对大鼠佐剂性关节炎(AA)的抑制作用。方法 36只雄性SD大鼠均分为6组，除正常组外，其余各组大鼠均制备AA模型。足容积法检测AA大鼠继发侧足爪容积；流式细胞术检测外周血CD4⁺及CD8⁺T细胞亚群的变化；免疫组织化学链霉卵白素-过氧化物酶法(SP)检测滑膜组织微血管密度(MVD)的改变。结果与模型组相比，索拉非尼组大鼠足爪容积下降，滑膜组织MVD减小，其中索拉非尼(20、40mg/kg)组可使外周血CD4⁺T细胞比例降低，CD8⁺T细胞比例增加，差异均有统计学意义(P<0.05)。结论索拉非尼具有抑制大鼠AA效应，该作用可能与索拉非尼引起AA大鼠外周血CD4⁺, CD8⁺T细胞亚群的偏移以及降低滑膜组织MVD有关。

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4. 颈椎曲度改变的眩晕患者危险因素分析

王英殷闯徐建可尉娜谭军

解剖学报 2018, 49 (5): 641-645. DOI: 10.16098/j.issn.0529-1356.2018.05.013

摘要（2681）

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目的分析合并颈椎生理曲度改变的中青年眩晕患者的相关危险因素。方法收集2016年6月～2017年3月在新乡医学院第三附属医院神经内科住院的中青年眩晕患者病历，共78例，其中男性32例，女性46例。根据是否有颈椎生理曲度改变分为颈椎生理曲度正常组（n=22）和颈椎生理曲度异常组（n=56），比较两组的既往病史、实验室检查，对结果进行统计学分析。结果空腹血糖（FBG）、糖化血红蛋白（HbA1c）、同型半胱氨酸（HCY）、吸烟史及职业组间差异有显著性（P＜0.05）；性别、年龄在两组之间差异无显著性（P＞0.05）。结论合并颈椎生理曲度改变的中青年眩晕患者中FBG、HbA1c、HCY、吸烟史、糖尿病及职业是常见的危险因素。

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5. 实验性糖尿病大鼠肺组织AGEs、TGF-β1、CTGF免疫组织化学分析

张宏 ;赵伟;王瑞琳 ;张宝生;徐延光;张廷威 ;刘昌起

2008, 39 (6): 910-914.

摘要（2626）

Objective To approach the changes of advanced glycosylated endproducts (AGEs), transforming growth factor(TGF-β1) and connective tissue growth factors(CTGF) in the lungs of experimental diabetic rats and their relationship. Methods Totally 48 male SD rats were divided into the diabetes mellitus（DM） group and the control group，with 24 rats in each group.The DM rat model was made by the injection of streptozocin (60mg/kg) into the caudal vein. The rats were killed and the lungs were taken at the end of the 4 th, 12 th and 20th weeks respectively after the models were established. The changes of AGEs,CTGF,TGF-β1 in rat lungs were observed with immunohistochemical assay and the image was analyzed. Results A great quantity of AGEs positive cells were observed in the alveolar epithelial cells, bronchial mucosal epithelium, angio-endothelial cells and smooth muscle cells of the DM rats. The average gray(AG) was lower than that of the controls（EM>P/EM><0.05） and decreased with the DM course（EM>P/EM><0.01）. In the 4_week DM rats, there were a few CTGF and TGF-β1 positive cells in the bronchial mucosal epithelium, angio-endotheliial cells and lung interstitial cells. In the 12_and 20_week DM rats, there were a great many CTGF and TGF-β1 positive cells. The AG was lower than that of the controls（EM>P/EM

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6. 兔卵巢组织冷冻移植后卵母细胞成熟及受精发育能力的研究

晁岚 ;邓晓惠;李晓梅;于璇;于红玲;甄军晖 ;傅庆诏

2008, 39 (5): 742-746.

摘要（2603）

Objective To investigate the effects of transplantation of frozenthawed ovarian tissues on the maturation, fertilization and further developmental potency of oocytes retrieved from grafts. Methods Twenty five New Zealand white female rabbits were divided into three groups randomly, group 1(EM>n/EM>=5), control group; group 2 (EM>n/EM>=10), fresh ovarian tissues were autologously transplanted into the mesometrium; and group 3(EM>n/EM>=10), frozenthawed ovarian tissues were autologously transplanted into the mesometrium. Three months after the transplantation, rabbits were stimulated with folliclestimulating hormone and oocytes were retrieved from antral follicles 13 hours after human chorionic gonadotropin injection. In vitro maturation (IVM) and intracytoplasmic sperm injection (ICSI) were performed to evaluate the fertility potency of the oocytes from frozen ovarian grafts. Results The number of retrieved oocytes in group 2 and 3 were lower than those of the control group (P<0.05); But no significant differences were observed between group 2 and group 3 (EM>P/EM>>0.05); There were no significant differences both in the percentage of immature oocytes and the maturation rate after IVM, among the 3 groups (EM>P/EM>>0.05); Also, among the 3 groups or in each group, the fertilization rate, cleavage rate and blastocyst formation rate showed no difference, no matter the oocytes matured EM>in vivo/EM> or EM>in vitro/EM> (EM>P/EM>>0.05); The blastocyst formation rate derived from oocytes that matured in vitro was significantly lower than oocytes that matured EM>in vivo/EM> (EM>P/EM><0.05). Conclusion

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7. 成年国人冠状动脉管径的CT解剖学研究

陈艳;韩萍;梁波;史河水;雷子乔;刘永华

2008, 39 (6): 936-940.

摘要（2591）

Objective To evaluate the CT features of the coronary artery in normal adults and to measure the diameter of coronary artery using 16-slice computed tomography. Methods 16-slice CT coronary angiography was performed in this study. Totally 104 cases whose coronary arteries were normal were divided into 3 groups according to age and dominant pattern of coronary artery. The diameter of right artery (RCA), including the proximal, middle and distant segments were measured by using CT, so was the diameter of left main artery (LM), left anterior descending artery (LAD) including the proximal, middle and distant segments, and left circumflex artery proximal and distant segments. Results The diameters of coronary arteries increased with the age. There was a difference of LM diameter between the elder group and the youth group and middle group. However there was no difference in the youth group and the elder group. No difference were detected from other coronary artery segment’s diameter. The diameter of LM in left dominant pattern was the largest, and it showed a difference when compared with the other two groups, bt there was no difference between the latter two groups. The diameters of LAD and LCX in left dominant pattern were the largest, and that of in right dominant pattern were the smallest. The diameter of LAD had no difference among the three groups. The diameter of LCX proximal segment had no difference between the balanced dominant pattern and right dominant pattern, but there was a difference compare to the left dominant pattern. The diameters of LCX distant segment showed differences between any two groups. The diameter of RCA in the right dominant pattern was the largest. The diameters of RCA proximal segments showed no difference between the balanced dominant pattern and left dominant pattern, and there was difference among any other groups.Conclusion MSCT can display coronary artery from any angle and position. The diameter of coronary artery segment increases with the increase of age. Coronary artery size measured by MSCT can provide valuable information for coronary disease.

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8. CD2相关蛋白在肾脏细胞中的分布及其在足细胞中的作用

姜华军;张春;朱忠华;刘建社;邓安国

2008, 39 (5): 688-692.

摘要（2562）

Objective To study the distribution of CD2-associated protein (CD2AP) in normal renal cell lines and its interaction with nephrin and F-actin in podocytes. Methods The human mesangial cells (HMC) and HK-2 were cultured in DMEM. Conditionally immortalized murine podocyte cell line was cultured in RPMI 1640 The expressions of CD2AP and nephrin in podocytes were examined by RT-PCR and Western blotting. The distribution of CD2AP in HMC, HK-2, differentiated and undifferentiated podocytes was observed by laser scanning confocal microscopy. The coexistence of CD2AP with nephrin and F-actin in undifferentiated podocytes were also detected. Results CD2AP was distributed within the cytoplasm and perinulcear region of HK-2 and undifferentiated podocytes, but was absent in HMC cells. Its distribution profile changed and presented as peripheral accumulation when podocytes were put into differentiationpermissive conditions. CD2AP was located together with nephrin and F-actin in podocytes. Conclusion CD2AP can be detected in epithelial-originated renal cells. The alteration of distribution profile of CD2AP indicates it may participate in the process of podocytes differentiation and be involved in the regulation of slit diaphragm and cytoskeleton.

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9. 骨形态发生蛋白4在人胚胎卵黄囊造血过程中的表达

王跃嗣;李建远;靳韶华

2008, 39 (5): 723-727.

摘要（2551）

Objective To further explore the mechanism by which bone morphogenetic protein 4（BMP-4）might be involved in hematopoietic differentiation of the yolk sac. We observed the expression of BMP-4,CD34, CD133 and tyrosine kinase receptors（KDR） in the blood island of the yolk sac at embryonic 3 to 8 weeks. Methods Gene expression was analyzed by RT-PCR and the presence of BMP-4, CD34, CD133 and KDR proteins was confirmed by immunohistochemistry in 57 human embryos. Results In the human yolk sac, we found that BMP-4 was expressed at high levels from the 16th day to the 7th week, and decreased quickly after week 7 The results showed that KDR, CD133 and CD34 largely appeared on the 21st and 30th day, then increased at the 6th week, and decreased quickly after week 7. Furthermore, Ihh，SCl, GATA-1, GATA-2 and PU.1 mRNAs showed that PU.1 was not expressed on the 16th day; however, other factors were expressed all the time. Conclusion The distribution of BMP-4,KDR,CD34,CD133 and transcription factors expression highly suggested that BMP-4 was secreted from the yolk sac which might exert its effects on the specification of human hemangioblast and hematopoietic ste

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10. 人胎盘CD133^{+细胞具有高增殖潜能集落形成细胞特性}

刘佳云;陈代雄;方宁;章涛;刘祖林;祁莹;万卫红;刘金伟

2008, 39 (6): 915-918.

摘要（2534）

Objective To investigate whether human placenta tissue (PT) CD133SUP>+/SUP> cells possess the high proliferative potential colony forming cells(HPP-CFC) and analyze its properties in order to verify that human PT contains primitive hematopoietic stem/progenitor cells(HSPC). Methods Single cell suspension liquid of human PT was prepared by mechanical method. After that the mononucler cells(MNC) from PT was separated by Histopaque-1007 agent. CD133SUP>+/SUP> cells contained in MNC were isolated and purified by magnetic activated cell sorting(MACS) method. After 28 days of HPP-CFC expansion culture of CD133SUP>+/SUP> cells, the frequency and morphology of HPP-CFC were counted and observed. Phenotypes of purified CD133SUP>+/SUP> cells and HPP-CFC were analyzed by flow cytometer(FCM). In parallel, human umbilical cord blood(UCB) samples underwent the same protocols for comparison. Results After 28 days of culture the expansion fold of PTSUP>-/SUP>CD133SUP>+/SUP> cells was 266, lower than that of UCB-CD133SUP>+/SUP> cells which was 362 (P<0.01). The number of HPP-CFC in CD133SUP>+/SUP> cells derived from human PT and UCB were (32.4±11.2)/5×10SUP>3/SUP> and (17.7±5.7)/5×10SUP>3/SUP> respectively, the number of the former obviously higher than that of the latter (P<0.01). FCM analysis results showed that, except for CD133SUP>+/SUP>CD34SUP>-/SUP> subset from UCB-CD133SUP>+/SUP> cells, the proportion of CD133SUP>+/SUP>CD34+, CD133SUP>-/SUP>CD34SUP>+/SUP> subsets both from PT- and UCB-CD133SUP>+ /SUP>cells and CD133SUP>+/SUP>CD34SUP>- /SUP>subset from PT-CD133SUP>+/SUP> cells were decreased through 4 weeks of culture BR>.Conclusion Human PT-CD133SUP>+ /SUP>cells can form HPP-

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11. FGA等15个短串联重复序列在融水县苗族群体中的遗传学分析

周丽宁;覃耀春;徐林;李松峰;邓琼英;龚继春

2008, 39 (5): 683-687.

摘要（2521）

P>Objective To investigate the distribution patterns of 15 short tandem repeats (STR) loci (TPOX，TH01，CSF1PO，D19S433，vWA，D18S51，D5S818，FGA，D8S1179，D21S11，D7S820，D3S1358，D13S317，D16S539，D2S1338 ) in Miao ethnic group of Rongshui County in Guangxi Province. Methods The sodium citratedblood specimens were collected from 208 healthy unrelated Miao individuals (man: 102, female: 106) in Rongshui County in Guangxi Province, and then the DNAs from the samples were extracted by phynolchloroform technique. The DNAs were amplified by using AmpFlSTR IdentifilerTM PCR Amplification Kit, and finally the data were detected with 3100 Genetic Analyzer. Result Altogether 5.20 alleles and 11.62 genotypes of 15 STR were found in 208 healthy unrelated Miao individuals of Rongshui County in Guangxi Province. The allele frequency and genotype frequency were 0.004 8-0.466 3 and 0.004 8-0.317 3 respectively. Total discrimination power wa

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12. 小鼠骨髓基质细胞和蚕丝丝素材料的体外生物相容性

吴红;胡楠;杨宇民;顾晓松

2008, 39 (6): 831-835.

摘要（2507）

Objective To investigate the biocompatibility of bone marrow stromal cells (BMSCs) of mice EM>in vitro/EM> with silk fibroin materials and to explore a novel scaffold material to fabricate tissue-engineered nerve with introduction of BMSCs. Methods BMSCs were typically isolated from other cells by adherence to plastic. The mice-derived bone marrow stromal cells were cultured on the substrate of silk fibroin fibers and the cell attachment and growth during culture was observed by using light and electron microscopy. Mice-derived BMSCs were also cultured in the silk fibroin extract fluid. The cell ultrastructure was observed by transmission electron microscopy. MTT test was used to detect cell viability in different media for 12, 24, 48, 72 hours and 7 days respectively (the test was repeated 12 times for each group). Flow cytometry was employed to detect BMSCs cell cycle and phenotypes (the test was repeated 3 times). Results BMSCs cells were tightly attached to the silk fibroin fibers and grew along the silk fibroin fibers; some of them enwrapped the silk fibroin fibers and they exhibited either a spherical or spindle shape. The results of transmission electron microscopy, MTT test and flow cytometry analysis showed that there was no significant difference between BMSCs cultured in the silk fibroin extract fluid and those in plain IMDM medium in their morphology, cell viability, proliferation and phenotypes.Conclusion These data indicate that silk fibroin has good biocompatibility with BMSCs and is also beneficial to the survival of BMSCs without exerting any significant cytotoxic effects on their phenotype; thus it’s a potential scaffold material to fabricate tissue-engineered nerve with introductio

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13. 骨髓基质细胞条件培养液调节神经干细胞分化有效成分的蛋白质微阵列分析

顾平;马芹颖;王彦永;温雅;王铭维;崔冬生 ;刘力 ;耿媛

2008, 39 (6): 845-849.

摘要（2503）

Objective To detect the cytokines in the bone marrow stromal cells (BMSCs) conditioned medium (neurobasal conditioned medium ,N-CM) that can regulate the differentiation of neural stem cells (NSCs) into high proportional neurons and explore the mechanism that BMSCs regulate the differentiation of NSCs.Methods The collected N-CM was divided into two parts(>5kD and <5kD)by means of ultrafiltration after misce bene. The two parts were used to culture NSCs separately, and the effective part that could regulate the differentiation of NSCs was detected by protein microarray analysis. Results The N-CM>5kD could promote the NSCs to differentiate into high proportional neurons, so this part was detected by protein microarray analysis, 7 cytokines CINC-3, CNTF, IFN-γ, IL-1α, MCP-1, TIMP-1and VEGF were detected to up-regulate 1.5 times compared with the neurobasal medium (molecular weight above 5kD). Conclusion The dissolvable molecules excreted by BMSCs could promote the NSCs to differentiate into neurons. Some cytokines with the molecular weight of above 5kD play a crucial role during this process.

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14. shRNAs 介导的EM>Smad2/EM> 基因在小鼠成纤维细胞NIH/3T3中表达的沉默

郑嵘;熊琪;蒋思文;左波;李凤娥;徐德全;任竹青;熊远著

2008, 39 (5): 661-665.

摘要（2502）

Objective To construct five shRNA-expression plasmids and to investigate the expression of EM>Smad2/EM> in TGFβ/ Smads signal transduction treated with shRNA-expression plasmid. Methods Five shRNA-EM>Smad2/EM> DNA sequences from mRNA sequence of mouse EM>Smad2/EM> gene were designed and synthesized. DNA oligonucleotides encoding an appropriate shRNA were inserted to shRNA expression vector respectively. Five shRNAEM>Smad2/EM> expression plasmids were obtained and then transfected into NIH/3T3 cells. The suppressed expression of EM>Smad2/EM> was assessed by RT-PCR and Westernblotting. Results The shRNA-expression plasmid numbered 2.4 could markedly reduce the expression of EM>Smad2/EM>. The suppression effect of the RNAipool composed of four different plasmids was more obvious than that of any single. Conclusion The shRNAexpression plasmids were successfully constructed, which could specifically and effectively suppress the expression of EM>Smad2

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15. 体外培养的人源神经干-祖细胞超微结构分析

赵耀东;张天一;黄强;王爱东;董军;兰青;秦正红;顾晓松

2008, 39 (5): 615-619.

摘要（2475）

Objective Neural stem cells have become a major concern in the current research of neuroscience, for they are involved in the neural injuries and recoveries, the origin of neural tumors, as well as other fields. The study on their ultrastructures, which is still limited at present, is indispensible. To offer more information is the aim of this paper. Methods Neural stem cells/progenitors from human fetal brain tissue were cultivated EM>in vitro/EM> and observed under a scanning electron microscope (SEM) and a transmission electron microscope (TEM). Results Neural stem cells/progenitors presented to be neurospherelike after days of culture EM>in vitro/EM>. The neruospheres were made up of neural stem cells/progenitors and nonfixiform material inside, and cells in neruospheres could be divided into lucent and dark ones according to electron densities. Between adjacent cells as well as on the cytoplasmic sides of the apposed plasma membranes, there were vesiclelike structures. Cell membrane fusions were also observed between some adjacent cells. Single neural stem cell /progenitor was spherical with rough surface under SEM. Many kinds of organellas, e.g. Golgi’s complex and endocytoplasmic reticulum, were underdeveloped in neural stem cells/progenitors, which generally had big, nuclei and scanty cytoplasm. The numbers, types and maturities of cellular organs in different cells were not always identical, which showed their heterogeneities. For instance, both neurofilaments and microtubules could only be observed in a few neural stem cells/progenitors； lysosomes were very abundant in some, but even hardly founded in others. What’s more, autophagosomes at different stages and in differernt formations could be seen in most cells. The nuclei, frenquently containing huge amounts of euchromatin and a small quantity of heterochromatin, mostly were globular, sometimes reniform or lobulated; most neural stem cells/progenitors had only one chromatospherite, seldom two or more, and sometimes no obvious chromatospherite could be seen. Conclusion Developed autophagosomes, vesiclelike structures between adjacent cells as well as on the cytoplasmic sides of the apposed plasma membranes and cellular membrane fusions could be seen in the human embryooriginated neural stem cells/progenitors and

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16. 实验性变态反应性神经炎周围神经轴膜离子通道的分布和作用

李建萍;吕传真

2008, 39 (5): 649-655.

摘要（2473）

Objective To reveal the distribution and effects of peripheral nerve axolemma ion channels in experimental allergic neuritis (EAN). Methods The alteration of Na+ and K+ channels on peripheral nerves (PNs) in the course of EAN was observed and the relationship between the channels and nerve conduction properties was analyzed by assessing histology and electrophysiology of PNs as well as clinical severity. Results Na+ and K+ channels obviously decreased on day 9 post immunization (p.i.), a time point of disease onset, and quickly became undetectable in next two weeks. Undergoing a slow and incomplete regeneration, neither of the channels regained the normal appearance on day 85 p.i. even if the clinical symptom disappeared several weeks before. Na+ and K+ channels had a synchronous development during disease course and remained a close correlation with the alteration of paranodal myelin. Electrophysiological abnormality kept consistent with the disturbance of PNs just in the acute period of EAN (923d p.i.) and the compound muscle action potential (CMAP) amplitude partly reflected the distribution of axolemma ion channels. Conclusion Loss of axolemma ion channels of PNs might be one of the reasons directly leading to the early symptoms of EAN. As a structural component, the channels were liable to damage and difficult to restore. The clustering and maint

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17. 强力霉素诱导表达脑啡肽细胞株的构建及其对慢性神经痛大鼠的镇痛作用

徐颖;田玉科;田学愎等

2006, 37 (6): 617-621.

摘要（2442）

P>Objective To establish an immortalized rat astrocyte strain (IAST) expressing enkephalin regulated by doxycycline (Dox) and observe its analysesic effect on rat chronic neuropathic pain. Methods Retrovirus infection method was employed to develop an immortalized rat astrocyte strain expression enkephalin regulated by doxycycline. hPPE gene expression level of IAST/TetOn/hPPE strain was detected by Real time PCR and radioimmunoas say. Its analgesic potential was investigated by mechanical paw withdrawal thresholds after these cells were implanted into the subarachnoid space of chronic constrictive injury (CCI) rats. The expression of Fos protein in the dorsal horn of spinal cord was determined by immunohistochemistry. Results An immortalized rat astrocyte strain secreting enkephalin under the control of doxycycline was established successfully. After transplantation of IAST/TetOn/hPPE cell into the subarachnoid space of chronic constrictive injury (CCI) rats, the sensitivity of mechanical allodynia and the expression of Fos protein were significantly decreased (P<005), so the transplantation of IAST/Tet On/hPPE cell alleviated significantly CCIinduced chronic neuropathic pain in rats and the analgesic effect was also able to be regulated by Dox.Conclusion An immortalized rat astrocyte strain expressing enkephalin regulated by Dox has been established, which may provide a new tool for regulatable

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18. 星形胶质细胞条件培养基对培养海马神经元GluR2和PICK1 mRNA表达的调控

刘子建;朱长庚;刘庆莹;魏瑛;王伟

2007, 38 (1): 2-5.

摘要（2437）

Objective To explore the regulating mechanism of the astrocytes on the expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) subunit in epileptogenesis. Methods The astrocytic conditional medium (ACM) was collected after being stimulated by glutamate, and then ACM was added to the cultured hippocampal neurons. The expression changes of neuronal GluR2 and protein interacting with C-kinase-1 (PICK1) mRNA were detected by RT-PCR. Results In the cultured hippocampal neurons, the GluR2 mRNA expression was significantly decreased at the 2nd, 8th, and 12th hours after the administration of ACM compared with that in the control group (P<0.05). However, the PICK1 mRNA expression was significantly increased at the same time points after the administration of ACM compared with that in the control group (P<0.05). The ionotropic glutamate receptor antagonists D-AP5 and CNQX could not fully block the action of ACM. Conclusion In epileptogenesis, the activation of astrocytes can downregulate the expression of neuronal AMPARs GluR2 subunit by upregul

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19. 慢性复合应激对大鼠海马生长休止蛋白7表达的影响

廖家万 ;胡红梅;钟敬亮;孙朝越;孙新明;彭建安;刘胜洪

2008, 39 (6): 826-830.

摘要（2429）

Objective To study the expression of growth-arrest-specific protein 7 (Gas7) in the hippocampus of rats after chronic multiple-stress. Methods thirty-six Wistar rats were randomly divided into two groups: the chronic multiple stressed group and the control group. Rats in the stressed group were administered with four kinds of stressors including vertical rotation，sleeping deprivation，restraint(6hours/day) and illumination irregularly and alternately for 6 weeks. Then the expression of Gas7 protein in the hippocampus of rats was assayed by immunocytochemical method and Western blotting technique respectively and apoptotic cells were observed. Results The expression of Gas7 was extensively found in the hippocampus of both the control group and the experimental group mainly in the endochylema and processes of the neurons in the CA1 area. Positive staining, average absorbance in the CA1 and dentate gyrus of the stressed rats were higher than that in the control group (P<0.05). There was no difference in the expressions in the CA3 areas in the two groups (P>0.05). Caspase-3 positive cells were also obserued in the stressed rats. Western blotting detection indicated that the expression of Gas7 in the stressed rats was significantly higher than that of the control group(P<0.05).Conclusion Gas7 may participate in protecting neurons in the stess and promote the neurogenesis an development of neurons.

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20. 叉头框C2在人胚淋巴管发生过程中的表达

田丽娜 ;邓伟国;邹积艳;马莉;丁言良;宋文龄;付艳

2008, 39 (6): 886-889.

摘要（2418）

Objective To study the human lymphangiogenesis in the early stage of embryo and the expression of forkhead box C2(FOXC2) in human lymphangiogenesis. Methods Lymphatic vessel endothelial haluronic acid receptor-1(LYVE-1) was used as the special marker of lymphatic vessels.Immunohistochemical and double immunofluorescence staining were used to detect the expressions of FOXC2 and LYVE-1 in lymphatic vessels of 85 human embryos of 5 to 11 weeks pregnancy and analyze the features of lymphatic vessel genesis and development. Results LYVE-1 was initially detected in lymphatic vessels in 7-week human embryos. The lymphatic vessel endothelial cells presented brown staining for LYVE-1 in jugular and thoracic region. LYVE-1 was also found to express in human embryonic mesentery lymphatic vessels on the 70th day of pregnancy. The expression of FOXC2 in human embryo was detected prior to that of LYVE-1 At the beginning of the 6th week of pregnancy, FOXC2 was obviously seen in human embryonic mesoderm mesenchyme.FOXC2 expressed not only in human embryonic lymphatic vessels,but also in the vertebral body,cardiovascular wall and other tissues.The expressions of FOXC2 and LYVE-1 were still seen in human embryonic lymphatic vessel endothelial cells after 80 days of pregnancy. Conclusion Lymphangiogenesis of human embryo begins between the 7th and 8th weeks of pregnancy. FOXC2 and LYVE-1 could have some relation with the human embryonic lymphatic vessel genesis and development.

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