Abstract:

Objective To investigate the features of CT reconstruction imags, expressions of genes associated with epithelial-mesenchymal transition(EMT) in lung adenocarcinoma with micropapillary pattern (MPP) and the relationships between them. Methods Thirty-seven cases of invasive lung invasive adenocarcinoma (IAC) with MPP, 80 cases without MPP and their corresponding para-cancerous tissues were selected. The clinicopathological features and features of CT reconstruction images were compared. Real-time PCR and Western blotting were used to detect the expression of EMT markers. Results In MPP group, the proportions of lymph node metastasis, spicule, pleural indentation, solid type and lobulation were higher（P<0.001）.In MPP group, the expressions of E-cadherin, β-catenin were decreased（P<0.05）, and the expressions of N-cadherin, vimentin,Snail and transforming growth factor(TGF)-β were increased（P<0.05）. In spicule group, the percentage of decreased expression of E-cadherin and β-catenin was higer, while the percentage of increased expression of vimentin was higher（P<0.05）. In the group of solid type, the percentage of decreased expression of E-cadherin was higher, while the percentages of increased expression of N-cadherin and vimentin were higher（P<0.05）. Conclusion Lung invasive adenocarcinoma has characteristics of CT reconstruction images and EMT changes. CT reconstruction images are associated with the expression of the related molecules associated of EMT.

Key words: Micropapillary pattern, Epithelial-mesenchymal transition, Lung adenocarcinoma, Real-time PCR, Western blotting, Human