三七总皂苷预处理对炎性内脏痛大鼠脊髓背角谷氨酰胺合成酶表达的影响

doi:10.16098/j.issn.0529-1356.2015.01.004

解剖学报 ›› 2015, Vol. 46 ›› Issue (1): 20-24.doi: 10.16098/j.issn.0529-1356.2015.01.004

三七总皂苷预处理对炎性内脏痛大鼠脊髓背角谷氨酰胺合成酶表达的影响

张仁丽¹万炜¹ 刘红¹曾杰² 彭田红¹ 刘政海^1*

1.南华大学医学院临床应用解剖研究室，湖南衡阳 421001; 2.长沙医学院生物学教研室，长沙 41021

收稿日期:2014-06-18 修回日期:2014-07-30 出版日期:2015-02-06 发布日期:2015-02-06
通讯作者: 刘政海 E-mail:46926537@qq.com
基金资助:
湖南省教育厅科学研究项目;湖南省自然科学基金

Panax notoginseng saponins pretreatment affects the inflammatory visceral pain via upregulation of the glutamine synthetase in the rat spinal dorsal horn

ZHANG Ren-li¹ WAN Wei¹LIU Hong¹ ZENG Jie²PENG Tian-hong¹LIU Zheng-hai ^1*

1. Discipline of Anatomy of University of South China, Hu’nan, Hengyang 421001, China; 2. Biology Department of Changsha Medical University, Changsha 41021, China

Received:2014-06-18 Revised:2014-07-30 Online:2015-02-06 Published:2015-02-06
Contact: LIU Zheng-hai E-mail:46926537@qq.com

摘要/Abstract

摘要：

目的探讨三七总皂苷（PNS）预处理调节脊髓背角谷氨酰胺合成酶（GS）表达，进而影响大鼠炎性内脏痛的病理生理机制。方法将27只SD大鼠随机分为正常对照组、PNS预处理组、溶媒对照组，给予相应处理15d后腹腔注射1%乙酸（10ml/kg）制作模型，每隔15min记录内脏痛指数；取小肠组织进行HE染色，光学显微镜下观察炎症反应；应用免疫组织化学技术检测胸段脊髓背角GS表达情况。结果溶媒对照组内脏痛指数明显高于PNS预处理组；腹腔注射乙酸后小肠组织都有明显的炎症反应，但两实验组黏膜下层厚度无明显差异；两实验组脊髓背角GS表达较正常组明显降低，但PNS预处理组脊髓背角GS表达明显高于溶媒对照组。结论 PNS预处理能上调脊髓后角GS的表达，从而缓解乙酸所致炎性内脏痛。

关键词: 炎性内脏痛, 三七总皂苷, 谷氨酰胺合成酶, 内脏痛指数, 免疫组织化学, 大鼠

Abstract:

Objective To explore the effect of panax notoginseng saponins (PNS) pretreatment on the expression of glutamine synthetase (GS) in the inflammatory visceral pain in the rat spinal dorsal horn. Methods Twenty-seven SD rats were randomly divided into normal group, PNS pretreatment group, and vehicle control group. Fifteen days after pretreatment, the model of inflammatory visceral pain was made by intraperitoneal injection of 1% acetic. The visceral pain index of experimental rat was record every 15 minutes. The small intestine tissue was stained with HE. The thoracic spinal cord slices were immunostained with anti-glutamine-synthetase antibody, then observed and compared under an optical microscope. Results The score result of the vehicle control group was significantly higher than that of the PNS pretreatment group. Compared with the normal group, the small intestine tissues of the two experimental groups showed significantly inflammatory response with thickness submucosa, but there was no significantly difference among experimental comparison groups. Compared with the normal group, the GS expression of the two experimental groups was reduced apparently, but the group of PNS pretreatment was significantly higher than the vehicle control group. Conclusion PNS pretreatment may affect the acid-induced inflammatory visceral pain via promoting the experience of GS in the spinal dorsal horn.

Key words: Inflammatory visceral pain, Panax notoginseng saponins, Glutamine synthetase, Visceral pain index, Immunohistochemistry, Rat

张仁丽万炜刘红曾杰彭田红刘政海*. 三七总皂苷预处理对炎性内脏痛大鼠脊髓背角谷氨酰胺合成酶表达的影响[J]. 解剖学报, 2015, 46(1): 20-24.

ZHANG Ren-li WAN Wei LIU Hong ZENG Jie PENG Tian-hong LIU Zheng-hai*. Panax notoginseng saponins pretreatment affects the inflammatory visceral pain via upregulation of the glutamine synthetase in the rat spinal dorsal horn[J]. AAS, 2015, 46(1): 20-24.

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三七总皂苷预处理对炎性内脏痛大鼠脊髓背角谷氨酰胺合成酶表达的影响

Panax notoginseng saponins pretreatment affects the inflammatory visceral pain via upregulation of the glutamine synthetase in the rat spinal dorsal horn

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