脑红蛋白在成年西门塔尔牛几种主要器官组织中的分布

doi:10.16098/j.issn.0529-1356.2015.01.006

解剖学报 ›› 2015, Vol. 46 ›› Issue (1): 32-37.doi: 10.16098/j.issn.0529-1356.2015.01.006

脑红蛋白在成年西门塔尔牛几种主要器官组织中的分布

高雪晋¹ 刘霞¹ 杨阳²梁春花² 杜晓华^2*

1.甘肃农业大学生命科学技术学院；2.动物医学院，兰州 730070

收稿日期:2014-09-09 修回日期:2014-10-27 出版日期:2015-02-06 发布日期:2015-02-06
通讯作者: 杜晓华 E-mail:duxh@gsau.edu.cn
基金资助:
甘肃省教育厅研究生导师项目;甘肃省高等学校基本科研业务费资助项目

Distribution of neuroglobin in several major organs and tissues of the adult simmental

GAO Xue-jin¹ LIU Xia¹ YANG Yang² LIANG Chun-hua² DU Xiao-hua ^2*

1. College of Life Science and Technology; 2.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received:2014-09-09 Revised:2014-10-27 Online:2015-02-06 Published:2015-02-06
Contact: DU Xiao-hua E-mail:duxh@gsau.edu.cn

摘要/Abstract

摘要：

目的探寻脑红蛋白（NGB）在成年西门塔尔牛不同组织细胞的分布特征。方法选择健康成年西门塔尔牛5只，利用免疫组织化学染色SP法观察NGB在其不同组织细胞的分布特征。结果结果显示，NGB在成年西门塔尔牛大脑和小脑皮质部、嗅球，海马，脊髓、肾上腺皮质部与髓质部、垂体、松果体、胰、睾丸、附睾、卵巢、子宫、胃、肠、肝、颌下腺、腮腺、心肌、脾脏、肺脏和肾脏等组织细胞中均有表达，NGB免疫阳性物质主要定位于细胞质。此外，本研究发现NGB在西门塔尔牛十二指肠段小肠腺细胞的表达强度要高于十二指肠腺细胞。结论 NGB在成年西门塔尔牛多种器官组织细胞中的广泛分布，提示NGB不仅在氧的利用及功能活动中发挥作用，在其他生理活动中可能也担负着重要的功能。

关键词: 脑红蛋白, 分布, 免疫组织化学, 西门塔尔牛

Abstract:

Objective To explore the localization of neuroglobin (NGB) in adult simmental tissues and cells. Methods Five health adult simmentals were used in this study. NGB expression in adult simmental tissues and cells was localized using the immunohistochemical SP technique. Results NGB-positive cells were distributed in tissues and cells of the cerebral and cerebellar cortex, olfactory bulb, hippocampus, spinal cord, adrenal cortex and medulla, hypophysis, pineal body, pancreas, testis, epididymis, ovary, uterus, stomach, intestines, liver, submandibular gland, parotid gland, cardiac muscle, spleen, lung and kidney, NGB-immunoreactive product was mainly located in the cytoplasm of these cells. In addition, the expression of NGB-positive in duodenum intestinal crypt cells was higher than that the expression of glandulae duodenales cells in simmental. Conclusion The widely expression of NGB in various organs, tissues and cells of the adult simmental suggest that NGB not only plays a role in the utilization of oxygen and physiological functions, there may have other important functions.

Key words: Neuroglobin, Distribution, Immunohistochemistry, Simmental

高雪晋刘霞杨阳梁春花杜晓华*. 脑红蛋白在成年西门塔尔牛几种主要器官组织中的分布[J]. 解剖学报, 2015, 46(1): 32-37.

GAO Xue-jin LIU Xia YANG Yang LIANG Chun-hua DU Xiao-hua*. Distribution of neuroglobin in several major organs and tissues of the adult simmental[J]. AAS, 2015, 46(1): 32-37.

参考文献

［1］Burmester T, Weich B, Reinhardt S, et al. A vertebrate globin expressed in the brain ［J］.Nature, 2000, 407(6803): 520-523. 
［2］Chen TF. A preliminary study of neuroprotective mechanism, expression, regulation and applications of neuroglobin (NGB) ［D］. Beijing: Academy of Military Medical Sciences，2005.(in Chinese)
陈婷方.脑红蛋白(NGB)神经保护机理与表达调控及其应用的初步研究(D).北京：中国人民解放军军事医学科学院， 2005.
［3］Chan AS, Saraswathy S, Rehak M, et al. Neuroglobin protection in retinal ischemia［J］. Invest Ophthalmol Vis Sci, 2012, 53(2): 704-711.
［4］Shang A, Liu K, Wang H, et al. Neuroprotective effects of neuroglobin after mechanical injury［J］. Neurol Sci, 2012, 33(3): 551-558.
［5］Qin H, Guo Y, Zhang C, et al. The expression of neuroglobin in astrocytoma［J］. Brain Tumor Pathol, 2012, 29(1): 10-16.
［6］Hota KB, Hota SK, Srivastava RB, et al, Neuroglobin regulates hypoxic response of neuronal cells through HIF-1α- and Nrf2-mediated mechanism［J］. J Cereb Blood Flow Metab, 2012, 32(6): 1046-1060.
［7］Wang HY, Deng MY, Wang J, et al. Expressions of neuroglobin gene in different cellular tissues of human body［J］. Journal of Applied Clinical Pediatrics, 2004, 19(4):278-280. (in Chinese)
王航雁, 邓美玉, 王静,等. 脑红蛋白基因在人体不同细胞组织中的表达［J］.实用儿科临床杂志，2004, 19(4): 278-280.
［8］Xu ShZh, Li JY, Ren HY, et al. The chinese simmental breeding and its progress［J］. Chinese Livestock and Poultry Breeding, 2008, 4(3): 13-15. (in Chinese)
许尚忠, 李俊雅, 任红艳, 等. 中国西门塔尔牛选育及其进展［J］. 中国畜禽种业, 2008, 4(3): 13-15.
［9］Reuss S, Saaler-Reinhardt S, Weich B, et al. Expression analysis of neuroglobin mRNA in rodent tissues［J］. Neuroscience, 2002, 115(3): 645-656.
［10］Huang B, Wang DL. The latest progress of neuroglobin［J］. Guangdong Medical Journal, 2010, 31(11): 1495-1497. (in Chinese)
黄飚, 王德林. 脑红蛋白的最新研究进展［J］. 广东医学, 2010, 31(11): 1495-1497.
［11］Greenberg DA, Jin K, Khan AA. Neuroglobin: an endogenous neuroprotectant［J］. Curr Opin Pharmacol, 2008, 8(1): 20-24.
［12］Sun Y, Jin K, Mao XO, et al. Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic-injury［J］.Proc Natl Acad Sci USA, 2001, 98(26): 15306-15311.
［13］Zhang KY, Niu SQ, Chen Y. The research of human nucleus orientation［J］. Journal of Jilin Medical College, 2008,29(3): 157-159. (in Chinese)
张阔野, 牛松青, 陈禹. 人脑神经核团空间定位研究［J］. 吉林医药学院学报, 2008, 29(3): 157-159.
［14］Laufs TL, Wystub S, Reuss S, et al. Neuron-specific expression of neuroglobin in mammals［J］. Neurosci Lett, 2004, 362(2): 83-86.
［15］Chen XQ, Qin LY, Zhang CG, et al. Presence of neuroglobin in cultured astrocytes［J］. Glia, 2005, 50(2): 182-186.
［16］Avivi A, Gerlach F, Joel A. Neuroglobin, cytoglobin, and myoglobin contribute to hypoxia adaptation of the subterranean mole rat spalax［J］. Proc Natl Acad Sci USA, 2010, 107(50): 21570-21575.
［17］Shang AJ, Zhou DB, Meng XH, et al. Distribution of neuroglobin in spinal cord of adult rat［J］. Acta Anatomica Sinica, 2006, 37(5): 492-494. (in Chinese)
尚爱加, 周定标, 孟祥辉, 等. 脑红蛋白在成年大鼠脊髓中的分布［J］. 解剖学报, 2006, 37(5): 492-494.
［18］Cao L, Liu X, Shi NN, et al. Distribution of neuroglobin in different tissues and cells of the adult yak ［J］. Acta Anatomica Sinica, 2013, 44(1): 71-75. (in Chinese)
曹亮, 刘霞, 石宁宁, 等. 脑红蛋白在成年牦牛不同组织细胞中的分布［J］. 解剖学报, 2013, 44(1): 71-75.
［19］Qin HJ, Zhang LSh, Wu N, et al. Study on the distribution and location of neuroglobin in human tissues and cells ［J］. Journal of Sichuan University(Medical Science Edition), 2008, 39(4): 609-611. (in Chinese)
秦豪杰, 张录顺, 武娜,等. 脑红蛋白在人体组织细胞的定位分布及意义［J］. 四川大学学报(医学版), 2008, 39(4): 609-611.
［20］Zhang ChG, Li L,Deng MY, et al. Coding region cDNA sequence cloning of rat neuroglobin gene, its polymorphism feature and tissue expression profile analysis［J］. Acta Genetica Sinica, 2001, 28(11): 997-1001. (in Chinese)
张成岗, 李林, 邓美玉,等.大鼠脑红蛋白基因编码区的克隆、多态性分析及该基因组织表达谱研究［J］.遗传学报,2001,28(11): 997-1001.
［21］Ma L, Bai ZhZh, Jin GE,et al. Tissue expression profile analysis of neuroglobin gene on Tibetan antelope［J］. Journal of Qinghai Medical College, 2012, 33(1): 12-15. (in Chinese)
马兰, 白振忠, 靳国恩, 等. 藏羚羊脑红蛋白基因的组织表达谱分析［J］. 青海医学院学报, 2012, 33(1): 12-15.

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脑红蛋白在成年西门塔尔牛几种主要器官组织中的分布

Distribution of neuroglobin in several major organs and tissues of the adult simmental

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