AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在大鼠肝再生过程中的表达变化

doi:10.16098/j.issn.0529-1356.2016.03.005

解剖学报 ›› 2016, Vol. ›› Issue (3): 315-322.doi: 10.16098/j.issn.0529-1356.2016.03.005

AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在大鼠肝再生过程中的表达变化

邢雪琨¹李梦华²徐存拴^3*

1.新乡医学院生命科学技术学院，河南新乡 453003; 2.山东大学国家糖工程技术研究中心，济南 250100; 3. 河南师范大学生命科学学院，河南新乡 453007

收稿日期:2015-10-19 修回日期:2015-12-11 出版日期:2016-06-06 发布日期:2016-06-06
通讯作者: 徐存拴 E-mail:csxu@x263.net
基金资助:
国家973 项目前期研究专项资助项目;河南省高等学校重点科研项目计划

Expression changes of AKT, ATM, D4-GDI and p53 cell apoptosis pathway genes in the process of rat liver regeneration

XING Xue-kun ¹ LI Meng-hua ² XU Cun-shuan^3*

1. Department of Life Science and Technology, Xinxiang Medical University, He’nan Xinxiang 453003, China; 2. National Glycoengineering Research Center, Shandong University, Ji’nan 250100, China; 3. College of Life Science, He’nan Normal University, He’nan Xinxiang 453007, China

Received:2015-10-19 Revised:2015-12-11 Online:2016-06-06 Published:2016-06-06
Contact: XU Cun-shuan E-mail:csxu@x263.net

摘要/Abstract

摘要：

目的探讨AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在肝再生（LR）过程中的表达变化。方法将大鼠随机分为实验组和对照组，每组6只，雌雄各半，用Rat Genome 230 2.0 芯片检测大鼠部分肝切除（PH）后不同恢复时间点4条细胞凋亡通路基因表达情况，并用生物信息学方法对其进行分析。结果 AKT、ATM、D4-GDI和p53 4条细胞凋亡通路中63、8、6和7个基因与肝再生相关。它们主要在肝再生启动阶段起始表达。表达的相似性分为均上调、上调占优势、均下调、下调占优势、上调和下调相近等5类，大多数基因表达加强，少数基因表达降低。它们表达的时间相关性分为11组。基因协同作用模型（Et）分析表明，AKT通路几乎在整个肝再生中抑制细胞凋亡；ATM、D4-GDI和p53 3条细胞凋亡通路几乎在整个肝再生中促进细胞凋亡。结论 AKT、ATM、D4-GDI和p53 4条细胞凋亡通路与再生肝生长、发育和肝量控制密切相关。

Abstract:

Objective To investigate the expression changes of four cell apoptosis pathway genes, AKT, ATM, D4-GDI and p53 in the process of liver regeneration.
Methods The rats were randomly divided into enperiment group and control group, 6 rats in each group, half male and half female. Genome Rat 230 2.0 was used to detect the expression of genes in four cell apoptosis pathways in rats after partial hepatectomy (PH), and the biological information method was used to analyze it. Results 63, 8, 6 and 7 genes were associated with liver regeneration in the four cell apoptosis pathways.They were mainly expressed at the start stage of liver regeneration. The similarity of their expression was including totally up regulation, up regulation advantage, totally down regulation, down regulation advantage, up regulation equal to down regulation of 5 categories. The expression of most genes in liver regeneration was enhanced, and the expression of few genes was decreased. Time correlation was divided into 11 groups. Analysis of the role of apoptosis pathway related genes in liver regeneration by using the gene synergy model (Et) indicated that AKT pathway inhibited the apoptosis of cells in the whole liver regeneration; ATM, D4-GDI and p53 three cell apoptosis pathway in the promotion of cell apoptosis in the whole liver regeneration. Conclusion Four apoptosis pathways, AKT, ATM, D4-GDI and p53, are closely related to the growth, development, and liver volume control of liver regeneration.

邢雪琨李梦华徐存拴*. AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在大鼠肝再生过程中的表达变化[J]. 解剖学报, 2016, (3): 315-322.

XING Xue-kun LI Meng-hua XU Cun-shuan *. Expression changes of AKT, ATM, D4-GDI and p53 cell apoptosis pathway genes in the process of rat liver regeneration[J]. AAS, 2016, (3): 315-322.

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AKT、ATM、D4-GDI和p53 4条细胞凋亡通路基因在大鼠肝再生过程中的表达变化

Expression changes of AKT, ATM, D4-GDI and p53 cell apoptosis pathway genes in the process of rat liver regeneration

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