解剖学报

解剖学报 ›› 2016, Vol. ›› Issue (3): 337-340.doi: 10.16098/j.issn.0529-1356.2016.03.008

• 细胞和分子生物学 • 上一篇    下一篇

突触孔蛋白和细胞色素C在棕榈酸诱导足细胞损伤中的表达及意义

戚昉1 臧东钰2 艾浩3 李晓明1,3*   

  1. 1. 锦州医科大学组织学与胚胎学教研室，辽宁 绵州 121001;2. 锦州医科大学附属第三医院胸外科，辽宁 绵州 121001; 3.辽宁省卵泡发育与生殖健康重点实验室， 辽宁 锦州 121001
  • 收稿日期:2015-10-26 修回日期:2016-01-05 出版日期:2016-06-06 发布日期:2016-06-06
  • 通讯作者: 李晓明 E-mail:lixiaoming68327@163.com
  • 基金资助:

    辽宁省高等学校优秀人才支持项目

Expression and significance of synaptopodin and cytochrome C in podocytes injuries induced by palmitate acid

QI Fang1 ZANG Dong-yu2 AI Hao3 LI Xiao-ming 1,3*   

  1. 1. Department of Histology and Embryology,Jinzhou Medical University, Liaoning Jinzhou 121001,China; 2. Thoracic Surgery Department of the Third Affiliated Hospital Jinzhou  Medical University, Liaoning Jinzhou 121001,China; 3. Follicular Development and Reproductive Health in Liaoning Province Key Laboratory, Liaoning  Jinzhou 121001,China

  • Received:2015-10-26 Revised:2016-01-05 Online:2016-06-06 Published:2016-06-06
  • Contact: LI Xiao-ming E-mail:lixiaoming68327@163.com

摘要：

目的 探讨细胞骨架蛋白突触孔蛋白（synaptopodin)和细胞色素C（Cyt C）在棕榈酸所致足细胞损伤中的表达及意义。 方法 体外培养小鼠肾足细胞，分为正常对照组和棕榈酸组。各组细胞分别培养 24h、72h和120h。应用MTT方法检测棕榈酸干预后足细胞活性的变化。应用免疫荧光及免疫印迹法检测棕榈酸干预后足细胞中synaptopodin和Cyt C蛋白的表达变化。 结果 与正常对照组相比，棕榈酸组足细胞活性明显下降，且呈时间依赖性。棕榈酸可上调足细胞中Cyt C蛋白的表达，并下调synaptopodin的表达。 结论 棕榈酸可诱导足细胞中Cyt C蛋白释放以及细胞骨架蛋白synaptopodin异常表达，可能参与了足细胞损伤。

Abstract:

Objective To investigate the expression and significance of synaptopodin and cytochrome C(Cyt C) in podocyte injuries induced by palmitate acid. Methods Podocytes were cultured and randomly assigned into two groups, normal group and palimitate acid group.The two groups were cultured for 24hours, 72hours and 120hours. Cell viability of podocytes induced by palmitate acid was examined by MTT. The expressions of synaptopodin and Cyt C of podocytes were examined by immunofluorescence and western blotting. Results Compared with the normal group, cell viability of podocytes was decreased in the palimitate acid group in a time dependence manner. The immunofluorescence and Western blotting results showed that expression of Cyt C was enchanced in podocytes induced by palmitate acid. The expression of synaptopodin was reduced in podocytes induced by palmitate acid. Conclusion Palmitate acid induces that Cyt C is released and synaptopodin is abnormally expressed in podocytes, which may result in podocyte injuries eventually.