母鼠注射麻黄素再灌胃中药混合液对仔鼠肝组织结构和抗氧化酶活性的影响

doi:10.16098/j.issn.0529-1356.2016.03.014

解剖学报 ›› 2016, Vol. ›› Issue (3): 368-373.doi: 10.16098/j.issn.0529-1356.2016.03.014

• 组织学胚胎学发育生物学 • 上一篇下一篇

母鼠注射麻黄素再灌胃中药混合液对仔鼠肝组织结构和抗氧化酶活性的影响

李重阳¹ 杨亚飞¹赵文成¹俞诗源^2.3*

1.甘肃政法学院公安分院，兰州 730070; 2.西北师范大学生命科学学院，兰州 730070; 3. 西北师范大学学报编辑部，兰州 730070

收稿日期:2015-11-25 修回日期:2016-02-09 出版日期:2016-06-06 发布日期:2016-06-06
通讯作者: 俞诗源 E-mail:syyu006@nwnu.edu.cn
基金资助:
甘肃省自然科学基金资助项目

Effects of the ephedrine injection followed by a stomach irrigation of Chinese medicine compound in the pregnant mouse on hepatic tissue structure and antioxidant enzymes activity of the filial mice

LI Chong-yang¹ YANG Ya-fei¹ ZHAO Wen-cheng¹ YU Shi-yuan ^2,3*

1. Department of Public Security，Gansu Political Science and Law Institute，Lanzhou 730070，China; 2. College of Life Science，Northwest Normal University，Lanzhou 730070，China; 3. Editoral Department of the University Journal， Northwest Normal University，Lanzhou 730070，China

Received:2015-11-25 Revised:2016-02-09 Online:2016-06-06 Published:2016-06-06
Contact: YU Shi-yuan E-mail:syyu006@nwnu.edu.cn

摘要/Abstract

摘要：

目的探讨母鼠注射麻黄素再灌胃中药混合液对仔鼠肝组织结构与抗氧化酶活性的影响。方法 30例受孕母鼠分为正常对照组、实验对照组及实验组3组，实验对照组和实验组母鼠连续腹腔注射0.2ml浓度为6.0 g/L麻黄素溶液（每天两次），正常对照组注射等量生理盐水，实验组母鼠在注射麻黄素1 h后再灌胃30.0g/L的中药混合液0.2ml，正常对照组和实验对照组灌胃等量的生理盐水。分别于分娩5、10、15 d时，用比色法测定仔鼠肝组织总抗氧化能力（T-AOC）、谷胱甘肽-S转移酶（GST）活力、丙二醛（MDA）含量及血浆谷丙转氨酶(ALT)活性的变化，用生物显微技术观察仔鼠肝组织结构的变化，用免疫组织化学法检测仔鼠肝组织TGF-β1表达的变化。结果实验对照组仔鼠肝组织T-AOC、GST活性明显降低，MDA含量升高，血浆ALT活性升高，肝组织有不同程度的损伤，肝组织TGF-β1表达增强(P<0.05或P<0.01)；而实验组仔鼠肝组织T-AOC、GST活性升高，MDA含量降低，血浆ALT活性降低，肝组织损伤有所改善，肝血窦扩张现象明显减轻，肝组织TGF-β1表达降低(P<0.05或P<0.01)。结论母鼠灌胃中药混合液可提高仔鼠肝细胞抗氧化酶活性，减少TGF-β1蛋白的表达，有效减轻母鼠因注射麻黄素对仔鼠肝组织的损伤。

Abstract:

Objective To explore effects of ephedrine injection followed by the stomach filling of Chinese medicine compound in the pregnant mouse on the development of the filial mice. Methods Totally 30 pregnant mice were used in the present study and divided into 3 groups，the natural control group，experimental control group and experimental group.The pregnant mice of experimental control group and experimental group were continuously intraperitoneally injected 6.0 g/L ephedrine (twice a day， 0.2ml each)，the natural control group was injected with the same amount of saline.The pregnant mice of experimental group were irrigated to stomach with 30.0 g/L Chinese medicine compound 0.2ml 1 hour after ephedrine injection.The natural control group and experimental control group were irrigated to stomach with same amount of saline.On day 5，10 and 15 after delivery，the activities of filial mice hepatic histological total antioxidant capacity（T-AOC），glutathione S-transferase（GST）and plasma alanine aminotransferase（ALT)，as well as content of malondialdehyde（MDA）MDA were determined by colorimetry，and the liver tissue structures of filial mice were observed by optical microscopy，the expression changes of TGF-β1 protein were measured by immunohistochemical staining. Results Compared with natural control group the activity of filial mice’s hepatic histological T-AOC，and GST of experimental control group were significantly reduced，while the content of MDA was increased significantly，the activity of plasma ALT was significantly increased，the filial mice’s liver tissue appeared varying degrees of damage，the expression intensity of TGF-β1 protein in liver tissue was significantly increased (P<0.05 or P<0.01).But the activity of T-AOC，GST of the filial mice’s liver tissue in the experimental group were increased significantly，the content of MDA was significantly decreased，and the activity of plasma ALT was significantly reduced.The pathological damage of liver was significantly improved，the expression of TGF-β1 protein was decreased (P<0.05 or P<0.01). Conclusion Ephedrine affects the histological structure and antioxidant enzymes activity and TGF-β1 expression of the liver.The female mice with stomach irrigation of Chinese medicine compound may enhance filial mice liver cells antioxidant enzymes activity，reduce TGF-β1 protein expression of liver tissue，and lighten the damage of filial mice liver tissue by ephedrine injecting of female mice.

李重阳杨亚飞赵文成俞诗源*. 母鼠注射麻黄素再灌胃中药混合液对仔鼠肝组织结构和抗氧化酶活性的影响[J]. 解剖学报, 2016, (3): 368-373.

LI Chong-yang YANG Ya-fei ZHAO Wen-cheng YU Shi-yuan*. Effects of the ephedrine injection followed by a stomach irrigation of Chinese medicine compound in the pregnant mouse on hepatic tissue structure and antioxidant enzymes activity of the filial mice[J]. AAS, 2016, (3): 368-373.

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母鼠注射麻黄素再灌胃中药混合液对仔鼠肝组织结构和抗氧化酶活性的影响

Effects of the ephedrine injection followed by a stomach irrigation of Chinese medicine compound in the pregnant mouse on hepatic tissue structure and antioxidant enzymes activity of the filial mice

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