解剖学报

解剖学报 ›› 2017, Vol. 48 ›› Issue (6): 758-760.doi: 10.16098/j.issn.0529-1356.2017.06.021

• 技术方法 • 上一篇    下一篇

球旁细胞光学显微镜下4种染色法结果观察

臧贵勇1* 张祥令2 胡蓉2   

  1. 1. 贵州省黔南民族医学高等专科学校解剖学与组织学胚胎学教研室， 贵州 都匀 558000; 2.贵州医科大学组织学胚胎学教研室，贵阳 550004
  • 收稿日期:2017-01-20 修回日期:2017-04-28 出版日期:2017-12-06 发布日期:2017-12-06
  • 通讯作者: 张祥令 E-mail:zhangxlyy@sina.com
  • 基金资助:
    贵州省科技厅联合基金项目资助

Four staining methods for observation of juxtaglomerullar cells under a light microscope

ZANG Gui-yong 1* ZHANG Xiang-ling2 HU Rong2   

  1. 1.Department of Anatomy and Histology，Qiannan Medical College for Nationalities of Guizhou，Guizhou Duyun 558000, China; 2.Department of Histology and Embryology，Guizhou Medical University， Guiyang 550004, China
  • Received:2017-01-20 Revised:2017-04-28 Online:2017-12-06 Published:2017-12-06
  • Contact: ZANG Gui-yong E-mail:zhangxlyy@sina.com

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摘要：

目的 探讨显示小鼠球旁细胞的适宜染色方法，更好地为教学和科研服务。 方法 取 20 只小鼠肾组织做石蜡切片，随机分为 4 组，分别用HE染色、高碘酸-Schiff 氏反应染色、改良结晶紫染色和免疫组织化学染色。 结果 HE染色和高碘酸-Schiff 氏反应染色不能显示肾球旁细胞；改良结晶紫染色和免疫组织化学均能较好地显示肾球旁细胞（胞质内有特征性的被染色的分泌颗粒）。 结论 改良结晶紫染色和免疫组织化学染色对球旁细胞有较好的染色效果，但改良的结晶紫染色更为简便、经济、稳定。

关键词: 肾球旁细胞, HE染色, 高碘酸-Schiff染色, 改良结晶紫染色, 免疫组织化学, 小鼠

Abstract:

Objective To explore an optimal staining method for observation of mouse kidney juxtaglomerullar cells. Methods Kidney tissues were harvested from 20 mice and prepared as paraffin sections. The sections were randomly divided to 4 groups for 4 different stainings. HE, PAS, modification crystal violet and immunohistochemistry staining, were used for the renal juxtaglomerular cellular secretive granula. Results Neither HE nor PAS methods was able to demonstrate the epithelium-like juxaglomerular cells. Both modification crystal violet method and immunohitochemistry staining methods demonstrated the epithelium-like juxaglomerular cells containing secretive granula. Conclusion Although both modification crystal violet and immunohistochemistry staining methods are able to show better staining result, the modification crystal violet method is more practical than the immunohistochemistry staining method , because of its convenience, easiness, low-cost and stability.

Key words: Renal juxtaglomerular cell, HE staining, Periodic acid-Schiff staining, Modification crystal violet staining, Immunohistochemistry, Mouse