丙戊酸钠诱导神经干细胞分化过程中肝配蛋白A3的表达

doi:10.16098/j.issn.0529-1356.2018.03.004

解剖学报 ›› 2018, Vol. 49 ›› Issue (3): 294-298.doi: 10.16098/j.issn.0529-1356.2018.03.004

丙戊酸钠诱导神经干细胞分化过程中肝配蛋白A3的表达

李雯何辉彭敏秦建兵施金洪金国华*

南通大学医学院人体解剖学系，江苏省神经再生重点实验室，神经再生协同创新中心，江苏南通 226001

收稿日期:2017-09-19 修回日期:2017-11-16 出版日期:2018-06-06 发布日期:2018-09-18
通讯作者: 金国华 E-mail:jguohua@ntu.edu.cn
基金资助:
江苏省自然科学基金青年基金项目;江苏省高等学校自然科学研究面上项目;南通大学研究生科技创新计划项目;南通大学引进人才科研启动基金项目;江苏省高等学校大学生创新创业训练计划项目;江苏高校优势学科建设工程资助项目

Expression of ephrin A3 during valproate induced neural stem cells differentiation

LI Wen HE Hui PENG Min QIN Jian-bing SHI Jin-hong JIN Guo-hua*

Department of Human Anatomy, Medical College of Nantong University, Jiangsu Key Laboratory of Neuroregeneration, Collaborative Innovation Center of Neuroregeneration, Jiangsu Nantong 226001, China

Received:2017-09-19 Revised:2017-11-16 Online:2018-06-06 Published:2018-09-18
Contact: JIN Guo-hua E-mail:jguohua@ntu.edu.cn

摘要/Abstract

摘要：

目的探讨丙戊酸钠（VPA）促进神经干细胞分化过程中肝配蛋白A3（Efna3）的表达以及Efna3在神经系统里的表达模式。方法分离培养SD大鼠海马神经干细胞，在VPA诱导分化后24 h和48 h，利用实时定量聚合酶链反应（Real-time PCR）、免疫印迹法（Western blotting）和免疫荧光技术检测Efna3的动态表达；Real-time PCR检测Efna3在成年SD大鼠各组织以及神经干细胞、星形胶质细胞和神经元中的表达水平。结果 VPA处理组与对照组相比，Efna3的表达量明显上升； Efna3在端脑和海马中呈现优势表达；Efna3在神经元中表达较高，而在星形胶质细胞中表达较低。结论 VPA促进神经干细胞分化可能与Efna3的表达上调有关。

关键词: 肝配蛋白A3, 丙戊酸钠, 神经干细胞, 神经元, 实时定量聚合酶链反应, 大鼠

Abstract:

Objective To investigate the expression of ephrin A3 (Efna3) in the process of neural stem cells differentiation induced by valproate (VPA) and the expression pattern of Efna3 in the nervous system. Methods The hippocampal neural stem cells of SD rats were isolated and cultured. At 24 hours and 48 hours after VPA inducement, the dynamic expression of Efna3 was detected by Real-time PCR, Western blotting and immunofluoresence assay. Real-time PCR was used to detect the expression of Efna3 in various tissues, as well as neural stem cells, astrocytes and neurons of adult SD rats. Results The expression of Efna3 increased significantly in the VPA group compared to the control group. Efna3 showed dominant expression in the cerebrum and hippocampus, and its expression was higher in neurons and lower in astrocytes. Conclusion The promotion of VPA to the differentiation of neural stem cells may be related to the upregulation of Efna3.

Key words: Ephrin A3, Valproate, Neural stem cell, Neuron, Real-time PCR, Rat

李雯何辉彭敏秦建兵施金洪金国华. 丙戊酸钠诱导神经干细胞分化过程中肝配蛋白A3的表达[J]. 解剖学报, 2018, 49(3): 294-298.

LI Wen HE Hui PENG Min QIN Jian-bing SHI Jin-hong JIN Guo-hua. Expression of ephrin A3 during valproate induced neural stem cells differentiation[J]. Acta Anatomica Sinica, 2018, 49(3): 294-298.

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丙戊酸钠诱导神经干细胞分化过程中肝配蛋白A3的表达

Expression of ephrin A3 during valproate induced neural stem cells differentiation

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