不良心理应激对人卵巢癌荷瘤裸鼠皮下移植瘤生长及表皮生长因子受体、磷酸化蛋白激酶B、血管内皮生长因子蛋白表达的影响

doi:10.16098/j.issn.0529-1356.2018.05.009

摘要/Abstract

摘要：

目的通过观察不良心理应激人卵巢癌荷瘤裸鼠皮下移植瘤的体积、重量和瘤体组织中表皮生长因子受体（EGFR）、磷酸化蛋白激酶B（p-Akt）、血管内皮生长因子（VEGF）蛋白表达情况的差异，探讨其对卵巢癌生长的影响及可能的分子机制。方法购置4～6周龄的雌性BALB/c裸鼠，称重，随机分两组，每组12只。A组：荷瘤组皮下移植人卵巢癌组织瘤块，不给予应激；B组：荷瘤+应激组皮下移植人卵巢癌组织瘤块，给予应激。定期测量皮下移植瘤的体积，结束应激实验后的第2天，处死全部组别（A组、B组）裸鼠，剥离肿瘤予以称重，利用免疫组织化学及Western blotting方法对A、B两组皮下移植瘤组织中的EGFR、p-Akt、VEGF蛋白表达情况进行检测。结果荷瘤+应激组皮下移植瘤体积及其重量显著高于荷瘤组（P＜0.01）；免疫组织化学实验提示，荷瘤+应激组中皮下移植瘤组织中的EGFR、p-Akt、VEGF蛋白表达水平高于荷瘤组（P<0.05）；Wester blotting提示，荷瘤+应激组皮下移植瘤组织中EGFR、p-Akt、VEGF蛋白表达水平显著高于荷瘤组（P<0.01）。结论不良心理应激可能通过EGFR及其介导的下游PI3K/Akt信号通路来促进卵巢癌的发生、发展。

关键词: 不良心理应激, 卵巢癌, 表皮生长因子受体, 磷酸化蛋白激酶B, 血管内皮生长因子, 免疫印迹法, 小鼠

Abstract:

Objective To explore the effect of adverse psychological stress on the growth and epithelial growth factor receptor(EGFR),phosphorylated protein kinase B(p-Akt) and vascular endothelial growth factor(VEGF) expressions of human ovarian cancer cells transplanted into nude mice. Methods Twenty-four female BALB/c(nu/nu)nude mice, aged 4 to 6 week-old, were randomly divided into two groups (12 mice per group): the tumor bearing group in which the human ovarian cancer tissue block was transplanted into nude mice subcutaneously without stress treatment, and the tumor bearing with stress treatment group in which the human ovarian cancer tissue block was transplanted into nude mice subcutaneously with stress treatment. After mice were sacrificed, tumors were separated and weighted. The protein expressions of EGFR, p-Akt and VEGF in the two groups were detected by immunohistochemistry and Western blotting method . Results The intensities of the immunohistochemical positive staining for EGFR,p-Akt,VEGF proteins in the tumor bearing with adverse stimulation group were higher than those in tumor bearing group. The results of Western blotting demonstrated that the intensities of the protein expressions for EGFR,p-Akt,VEGF in human bearing +stress group were higher than those in tumor bearing group. Conclusion The adverse psychological stress may promote the human ovarian cancer occurrence and development through by EGFR and its downstream PBK/AKT signal pathway.

Key words: Adverse psychological stress, Ovarian cancer, Epithelial growth factor receptor, Phosphorylated protein kinase B, Vascular endothelial growth factor, Western blotting, Mouse

金玲燕王弯弯张红陈琦. 不良心理应激对人卵巢癌荷瘤裸鼠皮下移植瘤生长及表皮生长因子受体、磷酸化蛋白激酶B、血管内皮生长因子蛋白表达的影响[J]. 解剖学报, 2018, 49(5): 617-623.

JIN Ling-yan WANG Wan-wan ZHANG Hong CHEN Qi.

Effect of adverse psychological stress on the growth and epithelial growth factor receptor, phosphorylated protein kinase B and vascular endothelial growth factor expressions of human ovarian cancer cells transplanted into nude mice

[J]. Acta Anatomica Sinica, 2018, 49(5): 617-623.

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