青海省妊娠高血压综合征患者血清瘦素水平及瘦素受体的基因多态性

doi:10.16098/j.issn.0529-1356.2018.06.020

解剖学报 ›› 2018, Vol. 49 ›› Issue (6): 814-818.doi: 10.16098/j.issn.0529-1356.2018.06.020

青海省妊娠高血压综合征患者血清瘦素水平及瘦素受体的基因多态性

庄文婷¹ 赵得雄²王香林¹李红荣³ 李长兴¹ 李福鑫¹ 孔得霞⁴ 李建华^1*

1.青海大学医学院基础医学部解剖学教研室，西宁 810001; 2.青海省红十字医院产科，西宁 810001; 3.甘肃省武威肿瘤医院外科，甘肃武威 733000; 4.西宁市第二人民医院产科,西宁 810001

收稿日期:2018-05-08 修回日期:2018-06-10 出版日期:2018-12-06 发布日期:2019-02-28
通讯作者: 李建华 E-mail:1184666039@qq.com
基金资助:
多因子基因多态性与青海藏族、汉族妇女妊娠高血压疾病相关性研究

Serum leptin and polymorphism of leptin receptor gene Gln223Arg of Qinghai patients with pregnancy-induced hypertension syndrome

ZHUANG Wen-ting¹ ZHAO De-xiong² WANG Xiang-lin¹ LI Hong-rong³ LI Chang-xing¹LI Fu-xin¹ KONG De-xia⁴ LI Jian-hua ^1*

1.Department of Human Anatomy, Basic Medicine, Medical College of Qinghai University, Xining 810001,China; 2.Department of Obstetrics, Qinghai Red Cross Hospital, Xining 810001,China; 3.Department of Surgery, Gansu Wuwei Tumor Hospital, Gansu Wuwei 733000,China; 4.Department of Obstetrics, the Second People’s Hospital of Xining City, Xining 810001,China

Received:2018-05-08 Revised:2018-06-10 Online:2018-12-06 Published:2019-02-28
Contact: LI Jian-hua E-mail:1184666039@qq.com
Supported by:
Association of Polymorphism Gene Polymorphisms with Pregnancy-induced Hypertension Disorders in Qinghai Tibetan and Han Women

摘要/Abstract

摘要：

目的探讨瘦素受体（LEPR）Gln223Arg（rs1137101）基因多态性与青海省妊娠高血压综合征之间的相关性。方法选取妊娠高血压综合征（PIH）患者107例，正常妊娠组116例，用ELISA法检测血清瘦素水平。以外周静脉血DNA为模板，采用聚合酶链反应限制性内切酶长度片段多态性（PCR-RFLP）对LEPR 基因Gln223Arg位点基因型进行分析。结果 PIH组血清瘦素水平高于对照组（P<0.05）。PIH组基因型频率GG型、GA型和AA型分别为49.5%、43.9%和6.5%，对照组分别为63.8%、32.8%和3.4%。PIH组和对照组LEPR基因Gln223Arg 位点等位基因G和A频率分布具有差异性。PIH组A等位基因频率高于正常妊娠组（χ²=4.60，P<0.05，OR=0.62，95%CI=0.40～0.96）。结论 LEPR基因Gln223Arg的A等位基因是PIH遗传易感基因之一，可能是PIH的潜在危险因素。

关键词: 妊娠高血压综合征, 瘦素, 瘦素受体, 基因多态性, 酶联免疫吸附测定, 人

Abstract:

Objective To investigate the relationship between polymorphism of leptin receptor(LEPR) gene Gln223Arg (rs1137101) and pregnancyinduced hypertension syndrome. Methods The study consisted of 107 patients of pregnancy-induced hypertension syndrome (PIH) subjects, 116 normal pregnancy subjects were studied to detect their sera level of leptin by ELISA. The LEPR Gln223Arg genotype was detected by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Results The PIH group sera leptin level was higher than control group(P<0.05).The frequences of GG,GA,AA genotype of LEPR Gln223Arg in PIH group were 49.5%,43.9% and 6.5%, in control group were 63.8%,32.8% and 3.4%. There was difference in frequency distribution of LEPR genotype between PIH group and control group. The frequency of A gene in PIH group was higher than the control group（χ²=4.60，P<0.05，OR=0.62，95% CI=0.40-0.96）.Conclusion The A allele of LEPR Gln223Arg might be a genetic marker and a latent risk factor for PIH recurrence.

Key words:

Pregnancy-induced hypertension syndrome, Leptin, Leptin receptor, Gene polymorphism, Enzyme-linked immunosorbent assay, Human

庄文婷赵得雄王香林李红荣李长兴李福鑫孔得霞李建华. 青海省妊娠高血压综合征患者血清瘦素水平及瘦素受体的基因多态性[J]. 解剖学报, 2018, 49(6): 814-818.

ZHUANG Wen-ting ZHAO De-xiong WANG Xiang-lin LI Hong-rong LI Chang-xing LI Fu-xin KONG De-xia LI Jian-hua . Serum leptin and polymorphism of leptin receptor gene Gln223Arg of Qinghai patients with pregnancy-induced hypertension syndrome[J]. Acta Anatomica Sinica, 2018, 49(6): 814-818.

参考文献

［1］Le J. Obstetrics and Gynecology［M］. 7th ed.Beijing: People’s Health Publishing House,2008:92-101.（in Chinese）

乐杰.妇产科学［M］.第7版.北京:人民卫生出版社,2008:92-101.

［2］Cao ZY. Chinese Obstetrics and Gynecology［M］. 3th ed. Beijing: People’s Medical Publishing House, 2014:503-521.(in Chinese)

曹泽毅. 中华妇产科学［M］.第3版. 北京: 人民卫生出版社，2014:503-521.

［3］Sheppard SJ, Khalil RA. Risk factors and mediators of the vascular dysfunction.associated with hypertension in pregnancy［J］. Cardiovasc Hematol Disord Drug Targets, 2010,10(1): 33-52. 

［4］Kamani HT,Wijesekara LI,Rohan S,et al. LEPR c.668A>G polymorphism in a cohort of Sri. Lankan women with pre-eclampsia/pregnancy induced hypertension: a case control study［J］. BMC Res Notes, 2012, 5:308.

［5］Jiang LY. The relationship between leptin receptor polymorphism Gln223Arg, leptin and hypertensive disorder complicating pregnancy［D］. Huazhong University of Science and Technology, 2006:1-35.(in Chinese)

姜李媛.瘦素、瘦素受体基因多态性与妊娠期高血压疾病发病关系的研究［D］.华中科技大学, 2006:1-35.

［6］Tateishi A, Ohira S, Yamamoto Y,et al. Histopathological findings of pregnancy-induced hypertension:histopathology of early-onset type reflects two-stage disorder theory［J］ Virchows Arch,2018,472(4):635-642.

［7］Steegers EA, Dadelszen P, Duvekot JJ, et al. Pre-eclampsia［J］. Lancet,2010, 376 (9741):631-644.

［8］Roberts JM, Hubel CA.The two stage model of preeclampsia: variations on the theme ［J］. Placenta, 2010, S32-37.

［9］Frank TS, Ana CP, Joey PG. Immune Mechanisms Linking Obesity and Preeclampsia［J］. Biomolecules, 2015, 5 (4): 3142-3176.

［10］Su ChF, Zhang YH. Leptin and cardiovascular diseases［J］. Shanxi Medical Journal, 2011, 40(4):368-370.(in Chinese)

苏春芳，张颜华.瘦素与心血管疾病［J］.山西医药杂志, 2011, 40(4)：368-370.

［11］Muy-Rivera M, Ning Y, Frederic IO, et al.Leptin，soluble leptin receptor and leptin gene polymorphism in relation to preeclampsia risk［J］. Physiol Res, 2005, 54 (2):167-174.

［12］Miehle K, Stepan H, Fasshauer M. Leptin, adiponectin and other adipocytes in gestational diabetes mellitus and preeclampsia［J］. Clin Endocrinol Oxf, 2012, (76):2-11.

［13］Wang YN. Research progress on the relationship between leptin receptor and pregnancy［J］. Chinese Journal of Birth Health & Heredity, 2013, 21(4): 8-10.(in Chinese)

王亚男. 瘦素受体与妊娠相关性研究进展［J］. 中国优生与遗传杂志, 2013, 21(4): 8-10.

［14］Abelenda M, Ledesma A, Rial E, et al. Leptin administration to cold-acclimated rats reduces both food intake and brown adipose tissue thermogenesis ［J］. J Ther Biol, 2003, 28: 525-530.

［15］Wang S, Qiao FY, Feng L. High leptin level and leptin receptor Lys656Asn variant are risk factors for preeclampsia［J］.Genet Mol Res, 2013, 12(3): 2416-2422.

［16］Guan LX, Gao L, Gao Y, et al. Association of leptin and leptin receptor gene polymorphisms with severe preeclampsia［J］. Chinese Journal of Medical Genetics, 2011, 28(5): 562-567.(in Chinese)

管立学, 高丽, 高颍,等. 瘦素及瘦素受体基因多态性与重度子痫前期发病的相关性［J］. 中华医学遗传学杂志, 2011, 28(5): 562-567.

［17］BoumaizaⅠ, Omezzine A, Rejeb J, et al. Relationship between leptin G2548A and leptin receptor Q223R gene polymorphisms and obesity and metabolic syndrome risk in Tunisian volunteers［J］. Genet Test Mol Biomarkers,2012, 16(7):726-733.

［18］Ptak A, Kolaczkowska E, Gregoraszczuk EL.Leptin stimulation of cell cycle and inhibition of apoptosis gene and protein expression in OVCAR-3 ovarian cancer cells［J］. Endocrine,2013,43(2):394-403.

［19］Jin N, Li G, Li H, et al. Association of leptin receptor gene polymorphisms with hypertension in Chinese population: a meta-analysis［J］.Journal of Southern Medical University, 2014,32(2):265-269.

金楠，李革，李会,等.瘦素受体基因多态性与中国人群高血压相关性的分析［J］. 南方医科大学学报, 2014, 34(2): 265-269.

［20］Pena Gd, Guimares AL, Veloso RR, et al. Leptin receptor Gln223Arg polymorphism is not associated with hypertension:a preliminary population-based cross-sectional study［J］.Cardiol Res Pract,2014,2014:879037.

［21］Rigó J, Szendei G, Rosta K, et al. Leptin receptor gene polymorphisms in severely pre-eclamptic women［J］. Gynecol Endocrinol, 2006， 22(9): 521-525.

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青海省妊娠高血压综合征患者血清瘦素水平及瘦素受体的基因多态性

Serum leptin and polymorphism of leptin receptor gene Gln223Arg of Qinghai patients with pregnancy-induced hypertension syndrome

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