两种冷冻方法冻融人卵巢组织异种移植后血管生成素-2表达变化的比较

doi:10.16098/j.issn.0529-1356.2018.06.022

解剖学报 ›› 2018, Vol. 49 ›› Issue (6): 825-829.doi: 10.16098/j.issn.0529-1356.2018.06.022

两种冷冻方法冻融人卵巢组织异种移植后血管生成素-2表达变化的比较

张娜^1* 李微¹乜照燕¹ 张辉²郝雅丽¹ 唐岚¹

1.河北医科大学第四医院生殖医学科，石家庄 050011; 2.河北医科大学第四医院妇产科，石家庄 050011

收稿日期:2018-02-12 修回日期:2018-04-21 出版日期:2018-12-06 发布日期:2019-02-28
通讯作者: 张娜 E-mail:13833116806@163.com
基金资助:
河北省重点研发计划项目

Comparison of angiopoietin-2 expression on thawed and heteroplastic human ovarian tissues between two freezing methods

ZHANG Na ^1* LI Wei¹ NIE Zhao-yan¹ ZHANG Hui² HAO Ya-li¹ TANG Lan¹

1.Department of Reproductive Medicine, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011，China; 2.Department of Obstetrics and Gynecology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China

Received:2018-02-12 Revised:2018-04-21 Online:2018-12-06 Published:2019-02-28
Contact: ZHANG Na E-mail:13833116806@163.com

摘要/Abstract

摘要：

目的通过检测血管生成素-2（Ang-2）的表达，探讨两种冷冻方法对人卵巢组织的冻融及异种移植后的效果。方法共收集16例人卵巢组织标本，每例分成2份，其中1份应用程序化冷冻方法进行冷冻，另1份应用玻璃化冷冻方法进行冷冻。解冻后分别移植到去卵巢的雌性裸鼠颈部皮下。32只SCID裸鼠随机分为两组：A组16只，移植以程序化方法冷冻复苏的人卵巢组织；B组16只，移植以玻璃化方法冷冻复苏的人卵巢组织。解冻后及移植6周后观察两组卵巢组织中始基卵泡的存活及颗粒细胞Ang-2的表达情况。结果解冻后，A组Ang-2的阳性率明显高于B组（P<0.05），始基卵泡正常率两组相比差别不明显（P＞0.05）。移植后，始基卵泡正常率及Ang-2的阳性率两组相比均无明显差别（P＞0.05）。解冻后和移植后相比，两组的Ang-2阳性率及A组的始基卵泡正常率差别均不明显（P＞0.05），B组移植后始基卵泡正常率明显低于解冻后（P<0.05）。结论与玻璃化冷冻相比，程序化冷冻对人卵巢组织始基卵泡颗粒细胞Ang-2的表达影响更小。将解冻后的人卵巢组织进行异种移植不影响Ang-2的表达。

关键词: 卵巢组织冷冻, 程序化冷冻, 玻璃化冷冻, 血管生成素-2, 免疫组织化学, 裸鼠

Abstract:

Objective To investigate the effect of thawing and xenotransplantation of two kinds of cryopreservation method on human ovarian tissue by detecting the expression of angiopoietin-2 (Ang-2). Methods Sixteen cases of human ovarian tissue specimens were collected, and each case was divided into two parts. One part was cryopreserved by programmed freezing and the other one was cryopreserved by vitrification. Thirty-two SCID nude mice were divided into two groups: group A were transplantated the thawed human ovarian tissue frozen by programming freezing, and group B, by vitrification. After thawing and 6 weeks after transplantation, the survival rate of the primordial follicles and the expression of Ang-2 in the granulosa cells were observed in the two groups. Results After thawing, the positive rate of Ang-2 in group A was significantly higher than that in group B (P<0.05), and there was no significant difference in the normal follicular rate between the two groups (P>0.05). After transplantation, there were no significant differences in the survival rate of primordial follicles and the positive rate of Ang-2 between the two groups (P>0.05). There were no significant differences in the positive rate of Ang-2 (P>0.05) in two groups and there was no But in group B, the survival rate of primordial follicles after transplantation was lower than after thawing (P<0.05). Conclusion Programmed freezing had less effect on the expression of Ang-2 in the primordial follicle granulosa cells of the human ovarian tissue than vitrification. The heterograft of thawed human ovarian tissue does not affect the expression of Ang-2.

Key words: Ovarian tissue cryopreservation, Programmed freezing, Vitrification, Angiopoietin-2, Immunohistochemistry, Nude mouse

张娜李微乜照燕张辉郝雅丽唐岚. 两种冷冻方法冻融人卵巢组织异种移植后血管生成素-2表达变化的比较[J]. 解剖学报, 2018, 49(6): 825-829.

ZHANG Na LI Wei NIE Zhao-yan ZHANG Hui HAO Ya-li TANG Lan. Comparison of angiopoietin-2 expression on thawed and heteroplastic human ovarian tissues between two freezing methods[J]. Acta Anatomica Sinica, 2018, 49(6): 825-829.

参考文献

［1］Rosendahl M, Schmidt KT, Ernst E, et al. Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique［J］. Reprod Bio Med Online, 2011, 22(2): 162-171.

［2］Wang X, Fang C, Di ChG, et al. Effect of vitrification on morphology and apoptosis of human ovarian cortex tissue［J］. Journal of Sun Yat-sen University(Medical Sciences), 2015, 36(4): 545-550. (in Chinese)

汪翔, 方丛, 狄春光，等. 玻璃化冷冻对人类卵巢皮质组织形态学和凋亡的影响［J］. 中山大学学报（医学科学版）, 2015, 36(4): 545-550.

［3］Gougeon A. Dynamics of follicular growth in the human: a model from preliminary results［J］. Hum Reprod, 1986, 1(2): 81-87.

［4］Chang HJ, Moon JH, Lee JR, et al. Optimal condition of vitrification method for cryopreservation of human ovarian cortical tissues［J］. J Obstet Gynaecol Res, 2011, 37(8): 1092-1101.

［5］Tait CR, Jones PF. Angiopoietins in tumours: the angiogenic switch［J］. J Pathol, 2004, 204(1): 1-10.

［6］Vates GE, Hashimoto T, Young W, et al. Angiogenesis in the brain during development: the effects of vascular endothelial growth factor and angiopoietin-2 in an animal model［J］. J Neurosurg, 2005, 103(1): 136-145.

［7］Nishigaki A, Okada H, Tsuzuki T, et al. Angiopoietin 1 and angiopoietin 2 in follicular fluid of women undergoing a long protocol［J］. Fertil Steril, 2011, 96(6): 1378-1383.

［8］Kim D, Lee J, Johnson AL. Vascular endothelial growth factor and angiopoietins during hen ovarian follicle development［J］. Gen Comp Endocrinol, 2016, 232(1): 25-31.

［9］Hayashi KG, Acosta TJ, Tetsuka M, et al. Involvement of angiopoietin-tie system in bovine follicular development and atresia: messenger RNA expression in theca interna and effect on steroid secretion［J］. Biol Reprod，2003, 69(6): 2078-2084.

［10］Choi WJ, Seok JS, Choi IY, et al. Expression of angiogenic factors in cryopreserved mouse ovaries after heterotopic autotransplantation［J］. Obstet Gynecol Sci, 2015, 58(5): 391-396.

［11］Sanfilippo S, Canis M, Smitz J, et al. Vitrification of human ovarian tissue: apractical and relevant alternative to slow freezing［J］. Reprod Biol Endocrinol, 2015, 13(1):67(p1-7).

［12］Herraiz S, Novella-Maestre E, Rodríguez B, et al. Improving ovarian tissue cryopreservation for oncologic patients: slow freezing versus vitrification, effect of different procedures and devices［J］. Fertil Steril, 2014, 101(3): 775-784.

［13］Liebenthron J, Dittrich R, Toth B, et al. Orthotopic ovarian tissue transplantation-results in relation to experience of the transplanting centers, overnight tissue transportation and transplantation into the peritoneum［J］. Hum Reprod, 2015, 30(1): i97-i98.

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两种冷冻方法冻融人卵巢组织异种移植后血管生成素-2表达变化的比较

Comparison of angiopoietin-2 expression on thawed and heteroplastic human ovarian tissues between two freezing methods

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