解剖学报 ›› 2018, Vol. 49 ›› Issue (6): 846-851.doi: 10.16098/j.issn.0529-1356.2018.06.026

• 综述 • 上一篇    下一篇

现代显微成像技术及其在细胞生物学中的应用

杨治河1 闫丽2 李红林3 蔡亚非4*   

  1. 1.滁州城市职业学院解剖学教研室,安徽 滁州 239000; 2.临沂市人民医院肿瘤科,山东 临沂 276003; 3.上海市第十人民医院中心实验室,上海 200072; 4.南京农业大学动物科技学院,南京 210095
  • 收稿日期:2018-05-07 修回日期:2018-08-05 出版日期:2018-12-06 发布日期:2019-02-28
  • 通讯作者: 蔡亚非 E-mail:caiyafeinau@163.com
  • 基金资助:
    “十一五”国家科技支撑计划项目

Progress of advanced microscopic imaging technology and its applications in cell biology

YANG Zhi-he1 YAN Li2 LI Hong-lin3 CAI Ya-fei 4*   

  1. 1.Department of Anatomy, ChuZhou City Vocation College, Anhui Chuzhou 239000, China; 2.Department of Radiation Oncology, Linyi People Hospital, Shandong Linyi 276003, China; 3.Experiment Center, Shanghai 10th Hospital, Shanghai 200072,China; 4.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2018-05-07 Revised:2018-08-05 Online:2018-12-06 Published:2019-02-28
  • Contact: CAI Ya-fei E-mail:caiyafeinau@163.com

摘要:

具有高分辨率、能活体观察和三维成像的现代显微成像技术的产生和发展,为细胞生物学研究提供了有效的工具。我们在本文中阐述了几个重要的现代显微成像技术原理和技术要点以及在细胞生物学中应用的现状,包括光学显微术、电子显微术和荧光探针技术及相关技术,并对现代显微成像术的发展进行了展望。

关键词: 显微成像术, 分辨率, 活体观察, 三维成像,

Abstract:

Advanced microscopic imaging technology is a powerful tool in cell biology research. It can be used to achieve three-dimensional imaging in vivo with high resolution. This paper introduces the principles and key points of these techniques and the current situation of their applicaions in cell biology, including optical microscopy, electron microscopy, fluorescent probes and their related techniques.Moreover, the prospect of advanced microscopic imaging technology is also discussed.

Key words: Microscopic imaging technology, Resolution, Live observation, Three-dimensional imaging, Human