解剖学报

解剖学报 ›› 2020, Vol. 51 ›› Issue (1): 128-131.doi: 10.16098/j.issn.0529-1356.2020.01.022

• 生物工程学 • 上一篇    下一篇

人体脱细胞脂肪组织的制备及组织学评价

赵宇1 范军2 柏树令2*   

  1. 1.中国医科大学附属盛京医院整形美容外科，沈阳 110004; 2.中国医科大学公共基础学院组织工程教研室，沈阳 110122
  • 收稿日期:2018-12-18 修回日期:2019-01-23 出版日期:2020-02-06 发布日期:2020-04-21
  • 通讯作者: 柏树令 E-mail:baishuling@qq.com
  • 基金资助:
    国家自然基金

Preparation and histological evaluation of human decellularized adipose tissue

ZHAO Yu1 FAN Jun2 BAI Shu-ling2*   

  1. 1.Department of Plastic Surgery, Shengjing Hospital, Affiliated Hospital of China Medical University, Shenyang 110004, China; 2.Department of Tissue Engineering, School of Fundamental Science, China Medical University, Shenyang 110122, China
  • Received:2018-12-18 Revised:2019-01-23 Online:2020-02-06 Published:2020-04-21
  • Contact: BAI Shu-ling E-mail:baishuling@qq.com
  • Supported by:
    National Natural Science Foundation of China

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摘要：

目的 通过为人体吸脂来源的脂肪组织脱细胞，评估脱细胞脂肪组织的组织学成分，探讨其作为组织工程支架材料的可能性。 方法 采用改良的脱细胞方法为5例人吸脂来源的脂肪组织脱细胞，观察制备过程的大体形态，对脱细胞脂肪进行HE染色、Masson染色、Gomori染色、油红O染色、DAPI染色，并对脱细胞组织中残留的DNA、胶原蛋白及糖胺聚糖含量进行定量检测。 结果 本实验制备的脱细胞脂肪，外观呈不透明的乳白色，无固定形态。HE染色和DAPI染色无可见细胞成分残留，Masson染色可见胶原纤维，Gomori染色可见网状纤维存在，油红O染色无可见脂质残留。组织中残留的DNA含量微小，胶原和糖胺聚糖含量均有所保留。 结论 应用结合物理化学和酶学的脱细胞方法，可以为人体的吸脂来源脂肪组织脱细胞，得到的脱细胞组织去除了脂肪组织中的细胞及脂质成分，保留了脂肪组织细胞外基质的组成成分。

关键词: 脂肪组织, 脱细胞, 支架, 细胞外基质, 组织学染色, 人

Abstract:

Objective To decellularize adipose tissue derived from human liposuction, evaluate the histological composition of human decellularized adipose tissue, and explore the possibility for it as tissue engineering scaffold materials.  Methods The adipose tissue derived from five human liposuction was decellularized by a series of physical chemistry and enzymology method . The general morphology of the decellularization process was observed. HE staining, Masson staining, Gomori staining, oil red O staining and DAPI staining were performed and the content of DNA, collagens and glycosaminoglycans remaining in the decellularized adipose tissue were quantitatively detected.  Results The decellularized adipose tissue was some kind of milky, soft, variable insoluble material. HE staining and DAPI staining showed no residual cell components. Masson staining showed the presence of collagen fibers. Gomori staining showed the presence of reticular fibers, and oil red O staining showed no visible lipid residues. The residual DNA content in the tissue is very tiny and the collagen and glycosaminoglycan contents were retained.  Conclusion The adipose tissue derived from the body’s liposuction can be decellularized by physical, chemical, and enzymatic method . The cells and lipid in the decellularized adipose tissue can be effectively removed, and the components of the extracellular matrix of the adipose tissues are mostly retained.

Key words: Adipose tissue, Decellularization, Scaffold, Extracellular matrix, Histological staining, Human

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