解剖学报

解剖学报 ›› 2020, Vol. 51 ›› Issue (1): 132-134.doi: 10.16098/j.issn.0529-1356.2020.01.023

• 技术方法 • 上一篇    下一篇

采用紫BL与蓝B2R对组织切片染色的观察

邵金辉* 冯桂花   

  1. 湖北文理学院医学院形态学部，湖北 襄阳 441053
  • 收稿日期:2019-02-22 修回日期:2019-05-30 出版日期:2020-02-06 发布日期:2020-04-21
  • 通讯作者: 邵金辉 E-mail:jhshao666@163.com

Observation of tissue slice staining with purple BL and blue B2R

SHAO Jin-hui* FENG Gui-hua   

  1. Department of Morphology, School of Medicine, Hubei University of Art and Science, Hubei Xiangyang 441053, China
  • Received:2019-02-22 Revised:2019-05-30 Online:2020-02-06 Published:2020-04-21
  • Contact: SHAO Jin-hui E-mail:jhshao666@163.com

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摘要：

目的 寻找制备简单、效果良好、来源丰富的组织切片染色剂。 方法 应用27种直接服装染料对小鼠肝脏组织切片进行染色效果观察，发现其中的紫BL和蓝B2R染色能够清晰显示中央静脉和肝细胞核等结构，再用紫BL和蓝B2R对心脏、肾脏和睾丸组织切片进行染色效果观察。 结果 紫BL和蓝B2R用蒸馏水溶解后即可使用，配制简单，在5 min内完成。它们对心脏、肾脏和睾丸组织切片染色后，心肌横纹、肾小囊腔、生精细胞等结构清晰可辨，与HE染色结果类似。 结论 紫BL与蓝B2R比苏木素制备简单，同时它们的组织切片染色效果良好，来源丰富。因此，可能部分替代苏木素。

关键词: 服装染料, 肝, 心, 肾, 睾丸, 组织切片, 染色, 小鼠

Abstract:

 Objective To find out much more simply prepared, effective and abundant tissue slice dye.  Methods Totally 27 kinds of directly used dress dye were tested to make mouse liver slice stained. Among them purple BL and blue B2R made hepatic structure including central vein and hepatic nucleus shown clearly. Then, the heart, kidney and testis slices of mouse were stained by purple BL and blue B2R.  Results The solution preparation of Purple BL and blue B2R was just dissolved in distilled water in five minutes, so they were very much simple and timesaving.After the heart, kidney and testis slices of mouse were stained by purple BL and blue B2R,the stained tissue structure including cardiac muscle cross striation,kidney capsule cavity and spermatogenic cells is clear and similar to tissue stained by HE.  Conclusion The effective dress dye purple BL and blue B2R are much more simply prepared and abundant than hematoxylin. So, it can be partly replaced by purple BL and blue B2R.

Key words: Dress dye, Liver, Heart, Kidney, Testis, Tissue slice, Staining, Mouse

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