人包皮组织脱细胞基质的制备与生物相容性

doi:10.16098/j.issn.0529-1356.2020.03.023

摘要/Abstract

摘要：

目的制备人包皮组织脱细胞基质移植物（AMGs），探讨其浸提液对兔尿道黏膜细胞毒性。方法制备人包皮组织脱细胞基质后，通过Masson 染色观察脱细胞后基质的表征，扫描电子显微镜观察其孔径大小。体外培养尿道黏膜细胞，采用免疫荧光鉴定其AE3表达。尿道黏膜细胞与AMGs 基质浸提液体外共培养，MTT法检测 AMGs 浸提液对尿道黏膜细胞毒性。结果 Masson 染色显示细胞去除干净，只有胶原蛋白构成的支架，扫描电镜显示支架的孔径为20～50 μm，分布均匀，形态良好。适当浓度的AMGs 基质浸提液对尿道黏膜细胞无明显毒性。结论人包皮组织脱细胞基质为理想的 AMGs 支架材料，制备的 AMGs 材料细胞毒性小，有一定的实用价值。

关键词: 组织脱细胞基质移植物, 尿道黏膜细胞, 生物相容性, 扫描电子显微术, 人

Abstract:

Objective To prepare human foreskin tissue acellular matrix graft(AMGs), and explore the toxicity of the extracts on rabbit urethral mucosal cells. Methods After preparing the decellularized matrix of human foreskin tissue, the characteristics of decellularized matrix were observed by Masson staining, and the pore size was observed by scanning electron microscope.

Results Masson staining showed that the cells were removed, and the scaffold was composed of collagen. Scanning electron microscopy showed that the pore size of the scaffold was about 20-50 μm, which was uniformly distributed. The appropriate concentration of AMGs matrix extract had no obvious toxicity to urethral mucosal cells. Conclusion The acellular matrix of human foreskin tissue is an ideal AMGs scaffold material. The prepared AMGs material has low cytotoxicity and has practical value.

Key words: Acellular matrix graft, Urethral mucosa cell, Biocompatibility, Scanning electron microscopy, Human

中图分类号:

R726.9

林俊山赵星李笃妙许雅丽. 人包皮组织脱细胞基质的制备与生物相容性[J]. 解剖学报, 2020, 51(3): 446-449.

LIN Jun-shan ZHAO Xin LI Du-miao XU Ya-li. Preparation and biocompatibility of acellular matrix grafts of human foreskin[J]. Acta Anatomica Sinica, 2020, 51(3): 446-449.

参考文献

［1］ Jang H, Ma LM, Zhou J, et al. Human foreskin acellular matrix graft: a good scaffold for urethral tissue engineering［J］. National Journal of Andrology, 2009，15（5）:409-412.（in Chinese）

姜华，马利民，周娟，等. 人包皮脱细胞基质组织工程化尿道的可行性研究［J］. 中华男科学杂志，2009，15（5）:409-412.

［2］ Wu YG, Liu CL. Application of acellular matrix in urethral repair［J/CD］. Chinese Journal of Clinician(Electronic), 2011，5(19): 5734-5737.（in Chinese）

吴意光，刘萃龙. 脱细胞基质在尿道修复中的应用［J/CD］.中华临床医师杂志(电子版)，2011，5(19): 5734-5737.

［3］ Zheng H, Xie W L, Huang J, et al. Study on the replacement of urethra with no cell matrix in heterogeneous bladder［J］. Chinese Journal of Experimental Surgery, 2005,22（5）:565-566.（in Chinese）

郑浩，谢文练，黄健，等. 异种膀胱无细胞基质替代尿道的研究［J］. 中华实验外科杂志，2005，22（5）:565-566.

［4］ Menetrey J，Unno-Veith F，Madry H，et al.Epidemiology and imaging of the subchondral bone in articular cartilage repair［J］.Knee Surg Sports Traumatol Arthrosc,2010,18(4): 463-471.

［5］ Knapik DM,Harris JD,Pangrazzi G,et al. The basic science of continuous passive motion in promoting knee health: a systematic review of studies in a rabbit model［J］. Arthroscopy,2013,29(10):1722-1731.

［6］ Kim JS, Choi JS, Cho YW. Cell- free hydrogel system based on a tissue- specific extracellular matrix for in situ adipose tissue regeneration［J］. ACS Appl Mater Interfaces, 2017,9(10):8581-8588.

［7］ Ma R, Li M, Luo J, et al. Structu ral integrity, ECM components and immunogenicity of decellularized laryngeal scaff old with preserved cartilage［J］. Biomaterials, 2013, 34(7): 1790-1798.

［8］ Crapo PM, Medberry CJ, Reing JE, et al. Biologic scaff olds composed of central nervous system extracellular matrix［J］. Biomaterials, 2012,33(13): 3539-3547.

［9］ Wang L, Johnson JA, Chang DW, et al. Decellularized musculofascial extracellular matrix for tissue engineering［J］. Biomaterials, 2013, 34(11):2641-2654.

［10］ Zhao YG, Li MM. Preparation of acellular matrix material and its application in the repair of articular cartilage injury［J］. Tissue Engineering Research in China, 2016,20(34):5051-5056.（in Chinese）

赵玉果，李明明. 脱细胞基质材料制备方法及在骨关节软骨损伤修复中的应用［J］. 中国组织工程研究, 2016,20(34):5051-5056.

［11］ Guo W, Liu S, Zhu Y, et al. Advances and prospects in tissue-engineered meniscal scaffolds for meniscus regeneration［J］. Stem Cells Int, 2015, 2015:517520.

［12］ Gilbert TW. Strategies for tissue and organ decellularization［J］. J Cell Biochem, 2012, 113(7): 2217-2222.

[1]	李游徐丽明张琦敏杨素芳卢春花 . Copine-3-增强型绿色荧光蛋白融合蛋白在细胞中的表达和定位[J]. 解剖学报, 2021, 52(3): 384-390.
[2]	孔吟皓徐龙飞王琪韩晶洪艳. 神经元素3过表达对诱导人脐带间充质干细胞向胰岛素分泌细胞分化的影响[J]. 解剖学报, 2021, 52(3): 398-404.
[3]	吴超李咏兰. 内蒙古蒙古族察哈尔部人体组成成分分析[J]. 解剖学报, 2021, 52(2): 300-305.
[4]	高秀丽岳丽玲赵月生刘立琨刘得水朱文斌周丽. 乳腺癌中蛋白高乙酰化/琥珀酰化水平及其同组蛋白2AX高表达的相关性[J]. 解剖学报, 2021, 52(2): 244-250.
[5]	杨秀琳海向军马力扬马斌窦春江. 甘肃汉族成人骨强度及其与体成分的关系[J]. 解剖学报, 2020, 51(6): 940-944.
[6]	李晶傅宁薇范宁马威徐飞. 云南省纳西、普米、傈僳族肥胖状况分析及受试者工作特征曲线对高血压的预警作用[J]. 解剖学报, 2020, 51(6): 945-950.
[7]	张咸鹏曲泉颖布尔兰 ·叶尔肯别克李文慧温有锋. 哈萨克族成人6种肥胖评价指标准确性比较[J]. 解剖学报, 2020, 51(6): 951-958.
[8]	鲁广建狄文玉张群妹焦路阳. 微小RNA-186-5p对肾癌细胞增殖与侵袭能力的影响[J]. 解剖学报, 2020, 51(6): 877-881.
[9]	杨得草刘程马集王梦远战军张宏权. 细胞分裂周期相关蛋白7通过增强细胞增殖和干性促进人乳腺癌进程[J]. 解剖学报, 2020, 51(6): 888-896.
[10]	王婷婷刘超怡李秀芬李娜刘安丽. 芸香苷逆转人大肠癌LoVo/5-氟尿嘧啶细胞耐药性及其机制[J]. 解剖学报, 2020, 51(6): 906-911.
[11]	张志宏张瑛孙静娴丁帅文马国军迟彦艳于胜波郑楠隋鸿锦 . 犬肌硬膜桥的扫描电子显微镜观察[J]. 解剖学报, 2020, 51(6): 929-933.
[12]	刘文芳. 微小RNA-423-5p靶向乙醛脱氢酶2减轻脂多糖诱导的人脐静脉血管内皮细胞损伤[J]. 解剖学报, 2020, 51(6): 868-876.
[13]	庄晗刘学玲秦春丽王辉李郁欣李文生史勇红. 早期帕金森病相关脑结构的定量磁化率成像[J]. 解剖学报, 2020, 51(5): 738-744.
[14]	栾宁安凤杨孙健斌王玉辉李丽娟骆岩林李涛朱丽 . 中鼻甲连续冠状断面解剖及3D数字重建[J]. 解剖学报, 2020, 51(5): 637-643.
[15]	张珂柯嘉陶立元潘滔马芙蓉. 颞骨高分辨率CT的圆窗解剖学特征及在人工耳蜗植入术中的意义[J]. 解剖学报, 2020, 51(5): 670-676.