解剖学报

解剖学报 ›› 2021, Vol. 52 ›› Issue (5): 675-680.doi: 10.16098/j.issn.0529-1356.2021.05.001

• 神经生物学 •    下一篇

早期活体判断新生小鼠缺氧-缺血性脑损伤程度

颜琳1 张如秋1 华海蓉1 王茜1 曾祥菲1 李娟娟2 江萍1* 李凡1,3*   

  1. 1.昆明医科大学基础医学院病理学与病理生理学系，昆明 650500; 2.昆明医科大学基础医学院人体解剖学与组织学胚胎学系，昆明 650500;3.云南大学医学院病理学与病理生理学系，昆明 650500
  • 收稿日期:2020-04-09 修回日期:2020-05-28 出版日期:2021-10-06 发布日期:2021-10-06
  • 通讯作者: 江萍;李凡 E-mail:leefan623@sina.com
  • 基金资助:
    CD200R对HIBD后脑内MC及PBMCs突触修剪的调控机制研究;天麻素通过Sirt3-PI3k/Akt 通路保护新生大鼠缺血缺氧性脑损伤的研究;MC活化与OPCs交互作用在新生鼠HI相关性脑损伤致髓鞘形成障碍中的影响及其机制研究;MDMs介导的突触修剪在HIBD所致行为异常中的作用机制研究

Judging the degree of brain injury after hypoxia-ischemia in living neonatal mice in the early stage

YAN Lin1 ZHANG Ru-qiu1 HUA Hai-rong1 WANG Qian1 ZENG Xiang-fei1 LI Juan-juan2 JIANG Ping1*  LI Fan1, 3*   

  1. 1.Department of Pathology and Pathophysiology School of Basic Medical Science, Kunming Medical University, Kunming 650500, China;  2.Department of Human Anatomy and Histology and Embryology,  School of Basic Medical Science, Kunming Medical University, Kunming 650500, China; 3.Department of Pathology and Pathophysiology, Medical College, Yunnan University, Kunming 650500, China
  • Received:2020-04-09 Revised:2020-05-28 Online:2021-10-06 Published:2021-10-06
  • Contact: JIANG Ping;LI Fan E-mail:leefan623@sina.com

PDF

摘要：

目的 探讨早期活体区分缺氧-缺血性脑损伤（HIBD）程度的方法，为后续研究不同程度HIBD的分子机制奠定基础。   方法 采用改良Rice-Vannucci法构建C57BL/6 J小鼠HIBD模型。模型建立后1 d和3 d经活体剪开小鼠头皮行脑组织大体观察，区分轻重损伤。其后运用2, 3, 5-氯化三苯基四氮唑（TTC）染色、激光散斑脑血流成像、HE染色、Fluoro-Jade B（FJB）染色及术前、术后体重差，验证活体观察脑损伤程度的可靠性。   结果 HIBD后1 d和3 d经活体剪开小鼠头皮行脑组织大体观察，可将其区分为轻度损伤（HI-M）组和重度损伤（HI-S）组。HI-S组损伤侧出现明显的梗死灶，脑血流量显著减少，大量神经元坏死，体重较术前显著降低。HI-M组损伤侧脑血流量仅在HIBD后3 d减少，形态学观察到损伤侧皮层和海马区神经元排列疏松，体重较术前降低。   结论 剪开头皮行脑组织大体观察是早期活体情况下区分HIBD轻度及重度脑损伤的可靠方法。

关键词: 早期, 活体, 缺氧-缺血性脑损伤, 大体观察, HE染色, 小鼠

Abstract:

Objective  To explore the method  of distinguishing the degree of hypoxic-ischemic brain damage (HIBD) in living mice in early stage, so as to lay a foundation for the follow-up study of the molecular mechanism of different degrees of HIBD.    Methods  The modified Rice-Vannucci method  was used to duplicate the HIBD model of C57BL/6 J mice. On the 1 day and 3 days after the model, the scalp of mice were cut and the brain tissue were observed to distinguish between mild and severe lesions in living mice, and then 2, 3, 5-triphenyltetrazolium chloride (TTC) staining, laser speckle cerebral blood flow imaging, HE staining, Fluoro-Jade B (FJB) staining and body weight difference before and after operation were used to verify the reliability of observation in living mice.
  Results  Through the gross observation of brain tissue in living mice, HIBD could be divided into mild injury (HI-M) group and severe injury (HI-S) group. On day 1 and day 3 after HIBD, a significant decrease in cerebral blood flow, obvious gray infarction and a large number of necrotic neurons were observed in the HI-S group, and the body weight was significantly lower than that before operation. In the HI-M group, the cerebral blood flow of the injured side decreased only on the 3rd day after HIBD, and the loose arrangement of neurons in the cortex and hippocampus of the injured side was observed morphologically. The body weight was lower than that before operation.    Conclusion  Gross observation of brain tissue by cutting the scalp is a reliable method  to distinguish mild and severe brain injury in the early stage of HIBD in living mice.

Key words: Early stage, Living body, Hypoxic-ischemic brain damage, General observation, HE staining, Mouse

中图分类号: