解剖学报

解剖学报 ›› 2021, Vol. 52 ›› Issue (5): 681-685.doi: 10.16098/j.issn.0529-1356.2021.05.002

• 神经生物学 • 上一篇    下一篇

右美托咪定通过抑制脊髓星形胶质细胞活化改善甲醛诱导的急性炎性痛

曾学晴1 杨彬2 周鑫3 袁婧3 谢志艳3 李威3 万炜3 刘志文2* 曹文宇3*   

  1. 1.南华大学衡阳医学院2018级临床医学专业，湖南 衡阳 421001; 2.南华大学附属第二医院麻醉科，湖南 衡阳 421001; 3.南华大学衡阳医学院应用解剖学与生殖医学研究所，湖南 衡阳 421001
     
  • 收稿日期:2020-02-24 修回日期:2020-04-29 出版日期:2021-10-06 发布日期:2021-10-06
  • 通讯作者: 刘志文;曹文宇 E-mail:1242461483@qq.com
  • 基金资助:
    湖南省教育厅优秀青年项目;湖南省自然科学基金省市联合项目;南华大学大学生研究性学习和创新性实验计划项目;南华大学大学生研究性学习和创新性实验计划项目;南华大学大学生研究性学习和创新性实验计划项目

Dexmedetomidine alleviates formaldehyde induced pain by inhibiting astrocytes activation in spinal cord

ZENG Xue-qing1 YANG Bin2 ZHOU Xin3 YUAN Jing3 XIE Zhi-yan3 LI Wei3 WAN Wei3 LIU Zhi-wen2* CAO Wen-yu3*   

  1. 1.Grade 2018 Clinical Medicine, Hengyang Medical School, University of South China, Hu’nan Hengyang 421001, China; 2.Depatment of Clinical Anesthesiology, the Second Affiliated Hospital of University of South China, Hu’nan Hengyang 421001, China;  3.Clinical Anatomy and Reproductive Medicine Application Institute, Hengyang Medical School, University of South China, Hu’nan Hengyang 421001, China
  • Received:2020-02-24 Revised:2020-04-29 Online:2021-10-06 Published:2021-10-06
  • Contact: LIU Zhi-wen;CAO Wen-yu E-mail:1242461483@qq.com

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摘要：

目的  探讨右美托咪定（DEX）在0.1%甲醛诱导的急性炎性痛中的作用。   方法  将30只ICR雌性小鼠随机分成生理盐水对照（NS）组、甲醛（F）组、右美托咪定+甲醛（DEX+F）组。向右后爪足底注射25 μl 0.4%甲醛建立小鼠急性炎性痛模型，在甲醛注射前1 h，DEX+F组小鼠腹腔注射DEX，NS组和F组小鼠腹腔注射等体积生理盐水。以小鼠舔咬足累计时间评价自发痛行为。采用免疫组织化学法和Western blotting检测脊髓星形胶质细胞标记物胶质纤维酸性蛋白（GFAP）的表达。   结果  自发痛评分结果显示，与NS组相比， F组小鼠出现典型的双相痛反应；与F组相比，DEX+F组小鼠的第Ⅰ时相痛（P<0.001）和第Ⅱ时相痛（P<0.001）累计时间明显减少。免疫组织化学结果显示，与NS组相比，F组小鼠脊髓后角GFAP阳性细胞数目明显增多（P<0.001）；与F组相比，DEX+F组小鼠脊髓后角GFAP阳性细胞数目明显减少（P<0.001）。Western blotting结果显示，与NS组相比， F组小鼠脊髓GFAP表达明显增加（P<0.05）；与F组相比，DEX+F组小鼠脊髓GFAP表达明显减少（P<0.05）。   结论  DEX可能是通过抑制小鼠脊髓星形胶质细胞的活化从而改善甲醛诱导的急性炎性痛。

关键词: 右美托咪定, 甲醛, 星形胶质细胞, 急性炎性痛, 免疫印迹法, 小鼠

Abstract:

Objective To investigate the effect of dexmedetomidine（DEX）on formaldehyde-induced acute inflammatory pain.    Methods  Thirty female ICR mice were randomly divided into three groups, including normal saline control (NS) group, formaldehyde（F）group and dexmedetomidine + formaldehyde (DEX+F) group. 0.1% formaldehyde solution was injected into subcutaneous tissue of mice right hind paw to establish acute inflammatory pain model. An hour before formalin injection, mice were intraperitoneal injected with DEX in DEX+F group, and mice were intraperitoneal injected with equal volume saline in NS group and F group. The expression of spinal cord glial fibrillary acidic protein (GFAP）, which is a astrocytes marker, was detected by immunohistochemistry and Western blotting.    Results  Spontaneous pain score showed that compared with the NS group, the F group had typical biphasic pain response; while compared with the F group, the DEX+F group showed a significant decrease in the first (P<0.001) and second phase pain (P<0.001). Immunohistochemical result  showed that compared with the NS group, the F group had an increase number of GFAP positive cells in the posterior horn of the spinal cord (P<0.001). However, the DEX+F group showed a decrease number of GFAP positive cells in the posterior horn of spinal cord compared with the F group (P<0.001). Western blotting result  showed that compared with the NS group, the F group had increased expression of GFAP in the spinal cord (P<0.05). Compared with the F group, the DEX+F group showed decreased GFAP expression in the spinal cord (P<0.05).    Conclusion  DEX may improve formaldehyde-induced acute inflammatory pain in mice by inhibiting the activation of astrocytes in the spinal cord.

Key words: Dexmedetomidine, Formaldehyde, Astrocyte, Acute inflammatory pain, Western blotting, Mouse

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