β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达

doi:10.16098/j.issn.0529-1356.2022.06.002

解剖学报 ›› 2022, Vol. 53 ›› Issue (6): 698-704.doi: 10.16098/j.issn.0529-1356.2022.06.002

β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达

范娅楠^1,2 李超红¹ 贾祥磊¹ 金家豪¹刘玉珍^1*

1.新乡医学院第一附属医院河南省神经修复重点实验室，河南卫辉 453100; 2.郑州市第六人民医院检验科，郑州 450000

收稿日期:2021-01-16 修回日期:2021-02-16 出版日期:2022-12-06 发布日期:2022-12-06
通讯作者: 刘玉珍 E-mail:yuzhenliu@xxmu.edu.com
基金资助:
国家自然科学基金面上项目;国家自然科学基金面上项目;新乡医学院第一附属医院青年基金项目;新乡医学院研究生科研创新支持计划

Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues

FAN Ya-nan^1,2 LI Chao-hong¹ JIA Xiang-lei¹ JIN Jia-hao¹ LIU Yu-zhen^1*

1.He’nan Key Laboratory of Neural Regeneration, the First Affiliated Hospital of Xinxiang Medical University, He’nan Weihui 453100, China; 2.Department of Clinical Laboratory, Zhengzhou Sixth People’s Hospital, Zhengzhou 450000, China

Received:2021-01-16 Revised:2021-02-16 Online:2022-12-06 Published:2022-12-06
Contact: LIU Yu-zhen E-mail:yuzhenliu@xxmu.edu.com
Supported by:
National Natural Science Foundation Project;National Natural Science Foundation Project;Youth Foundation Project of the First Affiliated Hospital of Xinxiang Medical College;Research Innovation Support Program for Graduate Students of Xinxiang Medical College

摘要/Abstract

摘要：

目的观察β淀粉样前体蛋白裂解酶1（BACE1）在大鼠颈上神经节的表达和定位，及慢性间歇性低氧（CIH）对BACE1表达水平的影响。方法采用RT-PCR、Western blotting和免疫组织化学法检测颈上神经节BACE1的表达及分布。16只雄性SD大鼠随机分为对照（control）组和CIH组，每组8只，模型制作2周，RT-PCR检测CIH对BACE1、过氧化物酶体增殖物激活受体γ辅激活因子1α（PGC-1α）mRNA水平的影响。结果 BACE1表达于大鼠颈上神经节，主要分布于卫星胶质细胞和神经纤维，而在血管、神经元和小强荧光（SIF）细胞不表达。CIH下调BACE1 mRNA水平，上调PGC-1α mRNA水平（P<0.01）。结论 BACE1定位于大鼠颈上神经节卫星胶质细胞和神经纤维；BACE1水平降低可能参与调节CIH诱导的颈上神经节突触的可塑性。

关键词: β淀粉样前体蛋白裂解酶1, 颈上神经节, 慢性间歇性低氧, 免疫印迹法, 大鼠

Abstract:

Objective To observe the expression and localization of β-site amyloid precursor protein cleaving enzyme 1 (BACE1) in rat superior cervical ganglion and the effect of chronic intermittent hypoxia (CIH) on BACE1 level. Methods The expression and distribution of BACE1 in superior cervical ganglion were detected by RT-PCR, Western blotting and immunohistochemistry. Totally 16 male SD rats were randomly divided into control group and CIH group, 8 rats in each group. After 2 weeks of modeling, the effect of CIH on BACE1 and peroxisome proliferators activated receptor gamma co-activator 1 alpha (PGC-1α) mRNA level was detected by RT-PCR.

Results BACE1 was expressed in rat superior cervical ganglion, and mainly distributed in satellite glial cells and nerve fibers, but not in blood vessels, neurons and small intesely fluorecent(SIF) cells. CIH down-regulated BACE1 mRNA level, but up-regulated PGC-1α mRNA level (P<0.01). Conclusion BACE1 is located in satellite glial cells and nerve fibers in the superior cervical ganglion of rats. The decreased level of BACE1 may be involved in the regulation of CIH-induced synaptic plasticity of superior cervical ganglion.

Key words: β-site amyloid precursor protein cleaving enzyme 1, Superior cervical ganglion, Chronic intermittent hypoxia, Western blotting, Rat

中图分类号:

范娅楠李超红贾祥磊金家豪刘玉珍. β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达[J]. 解剖学报, 2022, 53(6): 698-704.

FAN Ya-nan LI Chao-hong JIA Xiang-lei JIN Jia-hao LIU Yu-zhen. Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues[J]. Acta Anatomica Sinica, 2022, 53(6): 698-704.

参考文献

［1］Ahmad SS, Kamal MA. Current updates on the regulation of beta-secretase movement as a potential restorative focus for management of Alzheimer’s disease ［J］. Protein Pept Lett, 2019, 26(8):579-587.

［2］Gerald K. BACE1 Function and inhibition: implications of intervention in the amyloid pathway of Alzheimer’s disease pathology ［J］. Molecules, 2017, 22(10):1723-1742.

［3］Salman LA, Shulman R, Cohen JB. Obstructive sleep apnea, hypertension, and cardiovascular risk: epidemiology, pathophysiology, and management ［J］. Curr Cardiol Rep, 2020, 22(2):6-15.

［4］Wszedybyl-Winklewska M, Wolf J, Szarmach A, et al. Central sympathetic nervous system reinforcement in obstructive sleep apnoea［J］. Sleep Med Rev, 2018, 39:143-154.

［5］Kameda Y. Signaling molecules and transcription factors involved in the development of the sympathetic nervous system, with special emphasis on the superior cervical ganglion ［J］. Cell Tissue Res, 2014, 357(3):527-548.

［6］Liu Y, Ji ES, Xiang S, et al. Exposure to cyclic intermittent hypoxia increases expression of functional NMDA receptors in the rat carotid body ［J］. Appl Physiol, 2009, 106(1):259-267.

［7］St-Pierre J, Drori S, Uldry M, et al. Suppression of reactive oxygen species and neurodegeneration by the PGC-1 transcriptional coactivators［J］. Cell, 2006, 127(2):397-408.

［8］Katsouri L, Parr C, Bogdanovic N, et al. PPARγ co-activator-1α (PGC-1α) reduces amyloid-β generation through a PPARγ-dependent mechanism［J］. Alzheimers Dis, 2011, 25(1):151-162.

［9］Sadleir KR, Kandalepas PC, Buggia-Prévot V, et al. Presynaptic dystrophic neuritis surrounding amyloid plaques are sites of microtubule disruption, BACE1 elevation, and increased Aβ generation in Alzheimer’s disease［J］. Acta Neuropathol, 2016, 132(2):235-256.

［10］Fisette JF, Montagna DR, Mihailescu MR, et al. A G-rich element forms a G-quadruplex and regulates BACE1 mRNA alternative splicing ［J］. J Neurochem, 2012, 121(5):763-773.

［11］Hung CO, Coleman MP. KIF1A mediates axonal transport of BACE1 and identification of independently moving cargoes in living SCG neurons ［J］. Traffic, 2016, 17(11):1155-1167.

［12］Fazliogullari Z, Kilic C, Karabulut AK, et al. A morphometric analysis of the superior cervical ganglion and its surrounding structures ［J］. Surg Radiol Anat, 2016, 38(3):299-302.

［13］Takaki F, Nakamuta N, Kusakabe T, et al. Sympathetic and sensory innervation of small intensely fluorescent (SIF) cells in rat superior cervical ganglion ［J］. Cell Tissue Res, 2015, 359(2):441-451.

［14］Hanani M. Satellite glial cells in sympathetic and parasympathetic ganglia: In search of function ［J］. Brain Res Rev, 2010, 64(2):304-327.

［15］Farah MH, Pan BH, Hoffman PN, et al. Reduced BACE1 activity enhances clearance of myelin debris and regeneration of axons in the injured peripheral nervous system ［J］. Neuroscience, 2011, 31(15):5744-5754.

［16］Prabhakar NR, Dick TE, Nanduri J, et al. Systemic, cellular and molecular analysis of chemoreflex-mediated sympathoexcitation by chronic intermittent hypoxia［J］. Exp Physiol, 2007, 92(1):39-44.

［17］Hu H, Hicks CW, He W, et al. BACE1 modulates myelination in the central and peripheral nervous system ［J］. Nat Neurosci, 2006, 9(12):1520-1525.

［18］Tallon C, Farah MH. Beta secretase activity in peripheral nerve regeneration ［J］. Neural Regen Res, 2017, 12(10):1565-1574.

［19］Lu W, Kang J, Hu K, et al. The role of the Nox4-derived ROS-mediated RhoA/Rho kinase pathway in rat hypertension induced by chronic intermittent hypoxia ［J］. Sleep Breath, 2017, 21(3):667-677.

［20］Irrcher I, Ljubicic V, Hood DA. Interactions between ROS and AMP kinase activity in the regulation of PGC-1alpha transcription in skeletal muscle cells ［J］. Am J Physiol Cell Physiol, 2009, 296(1):C116-C123.

［21］Wang R, Li JJ, Diao S, et al. Metabolic stress modulates Alzheimer’s β-secretase gene transcription via SIRT1-PPARγ-PGC-1 in neurons ［J］. Cell Metab, 2013, 17(5):685-694.

［22］Gong B, Pan Y, Vempati P, et al. Nicotinamide riboside restores cognition through an upregulation of proliferator-activated receptor-γ coactivator 1α regulated β-secretase 1 degradation and mitochondrial gene expression in Alzheime’s mouse models［J］. Neurobiol Aging, 2013, 34(6):1581-1588.

［23］Christensen MA, Zhou W, Qing H, et al. Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by SP1［J］. Mol Cell Biol, 2004, 24(2):865-874.

［24］Lu W, Ma YY, Shao QQ, et al. ROS/p53/miR-335-5p/Sp1 axis modulates the migration and epithelial to mesenchymal transition of JEG-3 cells［J］. Mol Med Rep, 2020, 21(3):1208-1216.

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β淀粉样前体蛋白裂解酶1在大鼠颈上神经节组织中的表达

Expression of β-site amyloid precursor protein cleaving enzyme 1 in rat superior cervical ganglion tissues

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