心肌梗死动物模型的建立和应用

doi:10.16098/j.issn.0529-1356.2024.02.018

摘要/Abstract

摘要：

心肌梗死是严重的心血管疾病之一，患者最终死于心力衰竭或心律失常。近年来，心肌梗死治疗的研究取得了很大进展，特别是临床前实验研究。心肌梗死的实验研究常依赖于动物模型，故心肌梗死模型的成功建立对于探索修复梗死心肌的新技术和新措施具有重要的应用价值。本文中我们对心肌梗死模型建立的技术和应用策略作了综述。

关键词: 冠状动脉, 心肌梗死, 心肌再生, 血管新生, 动物模型

Abstract:

Myocardial infarction is one of the severe cardiovascular diseases. The patients with myocardial infarction die of heart failure or arrhythmia. In recent years, the studies in myocardial infarction therapies have advanced greatly, especially the preclinical experimental studies. The experimental studies of myocardial infarction often rely on animal models. Therefore, successful establishment of the myocardial infarction models has important application value in exploring the new techniques and measures for repairing the infarcted myocardium. In this paper, the techniques in establishment of the myocardial infarction models and strategies of their application are summarized.

Key words: Coronary artery, Myocardial infarction, Myocardial regeneration, Angiogenesis, Animal model

中图分类号:

R541.1
R322

王海杰谭玉珍. 心肌梗死动物模型的建立和应用[J]. 解剖学报, 2024, 55(2): 247-252.

WANG Hai-jie TAN Yu-zhen. Establishment and application of animal models of myocardial infarction[J]. Acta Anatomica Sinica, 2024, 55(2): 247-252.

参考文献

［1］Lindsey ML, Bolli R, Canty JM Jr, et al. Guidelines for experimental models of myocardial ischemia and infarction［J］. Am J Physiol Heart Circ Physiol, 2018, 314(4): H812-H838.

［2］Martin TP, MacDonald EA, Elbassioni AAM, et al. Preclinical models of myocardial infarction: from mechanism to translation［J］. Br J Pharmacol, 2022, 179(5):770-791.

［3］Jiang ZL, Hu HT. Anatomical observation on the coronary arteries of the rat heart［J］. Acta Anatomica Sinica, 1984, 15(2):136-142. (in Chinese)

姜宗来,胡海涛.大鼠冠状动脉的解剖观察［J］.解剖学报，1984，15（2）：136-142.

［4］Wu JH, Wang HJ, Tan YZ, et al. Characterization of rat very small embryonic-like stem cells and cardiac repair after cell transplantation for myocardial infarction［J］. Stem Cells Dev, 2012, 21(8):1367-1379.

［5］Wang H, Paulsen MJ, Hironaka CE, et al. Natural heart regeneration in a neonatal rat myocardial infarction model［J］. Cells, 2020, 9(1):229.

［6］Wang QL, Wang HJ, Li ZH, et al. Mesenchymal stem cell-loaded cardiac patch promotes epicardial activation and repair of the infarcted myocardium［J］. J Cell Mol Med, 2017, 21(9):1751-1766.

［7］Salto-Tellez M, Lim SY, El Oakley RM, et al. Myocardial infarction in the C57BL/6J mouse: A quantifiable and highly reproducible experimental model［J］. Cardiovasc Pathol, 2004, 13(2):91-97.

［8］Gao E, Lei YH, Shang X, et al. A novel and efficient model of coronary artery ligation and myocardial infarction in the mouse［J］. Circ Res, 2010, 107(12):1445-1453.

［9］Reichert K, Colantuono B, McCormack I, et al. Murine left anterior descending (LAD) coronary artery ligation: An improved and simplified model for myocardial Infarction［J］. J Vis Exp, 2017, (122): e55353.

［10］Lugrin J, Parapanov R, Krueger T, et al. Murine myocardial infarction model using permanent ligation of left anterior descending coronary artery［J］. J Vis Exp, 2019, (150): e59591.

［11］Zhang NK, Gao LR, Zhao L, et al. Study on the rapid establishment of myocardial infarction model in mice［J］. Transl Med J, 2020, 9(6):373-377. (in Chinese)

张宁坤，高连如，赵力，等.小鼠心肌梗死模型的快速制作方法研究［J］.转化医学杂志，2020，9（6）：373-377.

［12］Crick SJ, Sheppard MN, Ho SY, et al. Anatomy of the pig heart: comparisons with normal human cardiac structure［J］. J Anat, 1998, 193 ( Pt 1):105-119.

［13］Wang HJ, Tan YZh. Practical Heart Anatomy［M］. Shanghai: Fudan University Press, 2007:116-129. (in Chinese)

王海杰，谭玉珍.实用心脏解剖学［M］.上海：复旦大学出版社，2007：116-129.

［14］Weaver ME, Pantely GA, Bristow JD, et al. A quantitative study of the anatomy and distribution of coronary arteries in swine in comparison with other animals and man［J］. Cardiovasc Res, 1986, 20(12):907-917.

［15］Krombach GA, Kinzel S, Mahnken AH, et al. Minimally invasive close-chest method for creating reperfused or occlusive myocardial infarction in swine［J］. Invest Radiol, 2005, 40(1):14-18.

［16］Pérez de Prado A, Cuellas-Ramón C, Regueiro-Purriños M, et al. Closed-chest experimental porcine model of acute myocardial infarction-reperfusion［J］. J Pharmacol Toxicol Methods, 2009, 60(3):301-306.

［17］Bikou O, Watanabe S, Hajjar RJ, et al. A pig model of myocardial infarction: Catheter-based approaches［J］. Methods Mol Biol, 2018, 1816:281-294.

［18］Schüttler D, Tomsits P, Bleyer C, et al. A practical guide to setting up pig models for cardiovascular catheterization, electrophysiological assessment and heart disease research［J］. Lab Animal, 2022, 51(2):46-67.

［19］Koudstaal S, Jansen of Lorkeers S, Gho JM, et al. Myocardial infarction and functional outcome assessment in pigs［J］. J Vis Exp, 2014, (86):51296.

［20］Ellenbroek GH, van Hout GP, Timmers L, et al. Primary outcome assessment in a pig model of acute myocardial infarction［J］. J Vis Exp, 2016, (116):54021.

［21］Silvis MJM, van Hout GPJ, Fiolet ATL, et al. Experimental parameters and infarct size in closed chest pig LAD ischemia reperfusion models; lessons learned［J］. BMC Cardiovasc Disord, 2021, 21(1):171.

［22］Suzuki Y, Lyons JK, Yeung AC, et al. In vivo porcine model of reperfused myocardial infarction: in situ double staining to measure precise infarct area/area at risk［J］. Catheter Cardiovasc Interv, 2008, 71(1):100-107.

［23］Abdelhafez MM, Shaw J, Wilbs J, et al. Improvement of a closed chest porcine myocardial infarction model by standardization of tissue and blood sampling procedures［J］. J Vis Exp, 2018, (133): e56856.

［24］Skyschally A, Hagelschuer H, Kleinbongard P, et al. Larger infarct size but equal protection by ischemic conditioning in septum and anterior free wall of pigs with LAD occlusion［J］. Physiol Rep, 2019, 7 (19):e14236.

［25］Wang YL, Zhang GT, Wang HJ, et al. Preinduction with bone morphogenetic protein-2 enhances cardiomyogenic differentiation of c-kit+ mesenchymal stem cells and repair of infarcted myocardium［J］. Int J Cardiol 2018, 265:173-180.

［26］Zhou P, Yu SN, Zhang HF, et al. c-kit+VEGFR-2+ mesenchymal stem cells differentiate into cardiovascular cells and repair infarcted myocardium after transplantation［J］. Stem Cell Rev Rep, 2023, 19(1): 230-247.

［27］Zhang HF, Wang YL, Tan YZ, et al. Enhancement of cardiac lymphangiogenesis by transplantation of CD34+VEGFR-3+ endothelial progenitor cells and sustained release of VEGF-C［J］. Basic Res Cardiol, 2019, 114(6):43.

［28］Liu Z, Mikrani R, Zubair HM, et al. Systemic and local delivery of mesenchymal stem cells for heart renovation: challenges and innovations［J］. Eur J Pharmacol, 2020, 876:173049.

［29］McCall FC, Telukuntla KS, Karantalis V, et al. Myocardial infarction and intramyocardial injection models in swine［J］. Nat Protoc, 2012, 7(8):1479-1496.

［30］Guo HD, Wang HJ, Tan YZ, et al. Transplantation of marrow-derived stem cells carried in fibrin improves cardiac function after myocardial infarction［J］. Tissue Eng Part A, 2011, 17(1-2): 45-58.

［31］Li ZH, Wang YL, Wang HJ, et al. Rapamycin-preactivated autophagy enhances survival and differentiation of mesenchymal stem cells after transplantation into infarcted myocardium［J］. Stem Cell Rev Rep, 2020, 16(2):344-356.

［32］Zhang GT, Tan YZh, Wang HJ, et al. Effects of marrow-derived cardiac stem cell transplantation after myocardial infarction in rats［J］. Chinese Journal of Cardiology, 2007, 35(10):940-944. (in Chinese)

张贵焘，谭玉珍，王海杰，等.骨髓源性心肌干细胞移植治疗心肌梗死的实验研究［J］.中华心血管病杂志，2007，35（10）：940-944.

［33］Wang YL, Yu SN, Shen HR, et al. Thymosin β4 released from functionalized self-assembling peptide activates epicardium and enhances repair of infarcted myocardium［J］. Theranostics, 2021, 11(9):4262-4280.

［34］Tan YZ, Shen HR, Wang YL, et al. Retinoic acid released from self-assembling peptide activates cardiomyocyte proliferation and enhances repair of infarcted myocardium［J］. Exp Cell Res, 2023, 422(1):113440.

［35］Borrelli MA, Turnquist HR, Little SR. Biologics and their delivery systems: Trends in myocardial infarction［J］. Adv Drug Deliver Rev, 2021, 173:181-215.

［36］Wang GD, Tan YZ, Wang HJ, et al. Autophagy promotes degradation of polyethyleneimine-alginate nanoparticles in endothelial progenitor cells［J］. Int J Nanomed 2017, 12: 6661-6675.

[1]	李允争孙秋颖唐中生朱世杰 . 穴位埋线对血管性痴呆大鼠学习记忆功能及海马微血管新生的影响[J]. 解剖学报, 2024, 55(2): 150-157.
[2]	宋慧芳谈佳音刘阳张卫国郭蕊 . 低氧预处理通过激活低氧诱导因子1α增强老年人骨髓间充质干细胞促血管新生能力#br# #br# [J]. 解剖学报, 2022, 53(1): 35-41.
[3]	张苏欣孙艳荣王文娟孙昊哲胡耀文秦丽华. Nogo在心脏中的分布及其在心血管疾病中的作用[J]. 解剖学报, 2021, 52(3): 495-498.
[4]	刘敏曾云祝珊珊巫佳翠高洪泉伊雪. 蛋白激酶CβⅡ诱导上皮-间质转化及血管新生促进肝癌发展[J]. 解剖学报, 2020, 51(6): 912-918.
[5]	王立友张冬颖 . 过表达肝/骨/肾型碱性磷酸酶对小鼠急性心肌梗死后心室重塑的影响[J]. 解剖学报, 2020, 51(3): 431-436.
[6]	张铁山娄丽芳郑琳琳马忠良徐影王政徐高磊张振华 . 冠状动脉的起源与意义[J]. 解剖学报, 2019, 50(1): 87-90.
[7]	杨黎晓任明芬郭志坤. 大鼠急性心肌梗死后干细胞抗原-1和Nanog阳性细胞的动态变化[J]. 解剖学报, 2017, 48(4): 457-462.
[8]	蔡新华* 蔡庆慧张永春田香勤. 异丙肾上腺素诱导大鼠左心室心肌损伤的自发再生[J]. 解剖学报, 2015, 46(6): 824-831.
[9]	左红波李辞霞郭志坤*. 大鼠心肌梗死后心功能变化与新生血管的关系[J]. 解剖学报, 2014, 45(6): 835-840.
[10]	张丹王海杰* 谭玉珍王强利伍金红李志华权哲 . 纤维蛋白凝胶承载内皮祖细胞移植梗死心肌后细胞自噬变化[J]. 解剖学报, 2013, 44(2 ): 194-198.
[11]	田国忠* 魏罡李梅秀钟震亚朱金玲李艳君杨宇魏伟. 葛瑞林对心肌梗死致心力衰竭大鼠保护作用及生长分化因子-15的影响[J]. 解剖学报, 2013, 44(2 ): 274-278.
[12]	王晓冰;郭志坤 . 乳鼠心肌细胞移植对兔急性心肌梗死区微血管密度的影响 [J]. , 2009, 40(5): 803-806.
[13]	范卫君;刘远健;袁知东;江锦赵;刘晓杰;王成林;刘鹏程. 成人冠状动脉解剖的三维CT研究[J]. , 2009, 40(2): 332-336.
[14]	陈艳;韩萍;梁波;史河水;雷子乔;刘永华. 成年国人冠状动脉管径的CT解剖学研究[J]. , 2008, 39(6): 936-940.
[15]	尹洪萍;朱有法;林国华. 弹性纤维参与大鼠心肌梗死后重构的形态学观察[J]. , 2008, 39(4): 607-610.