解剖学报

›› 2010, Vol. 41 ›› Issue (03): 339-343.doi: 10.3969/j.issn.0529-1356.2010.03.002

• 论著 • 上一篇    下一篇

碱性成纤维细胞生长因子和表皮生长因子联合诱导骨髓基质细胞分化形成神经球

杨琴1;李琦1;曾志磊2 ;李傲3 ;谢鹏1*;杨军1;任俊伟1   

  1. 1.重庆医科大学附属第一医院神经内科，重庆 400016; 2. 郑州市中心医院神经内科，郑州 450007;3.重庆工学院生物工程学院，重庆 400050
  • 收稿日期:2009-03-13 修回日期:2009-05-19 出版日期:2010-06-06
  • 通讯作者: 谢鹏

Differentiation of bone marrow stromal cells into neurospheres induced by basic fibroblast growth factor combined with epidermal growth factor in rats

  1. 1.Department of Neurology, the First Hospital Affiliated, Chongqing Medical University, Chongqing 400016, China;2.Department of Neurology, Zhengzhou Central Hospital, Zhengzhou 450007, China;3.College of Bioengineer, Chongqing Institute of Technology, Chongqing 400050, China
  • Received:2009-03-13 Revised:2009-05-19 Online:2010-06-06
  • Contact: XIE Peng

关键词: 骨髓基质细胞, 碱性成纤维细胞生长因子, 表皮生长因子, 神经球, 分化, 免疫荧光, 免疫印迹法, 大鼠

Abstract: P>Objective To observe whether bone marrow stromal cells(MSCs) can be induced to form neurospheres by basic fibroblast growth factor(bFGF) combined with epidermal growth factor (EGF) in rats. Methods MSCs were cultured with whole bone morrow. Effect of bFGF and EGF, inducing MSCs to differentiated into neurospheres was studied by morphology, immunofluorescence, and Western blotting. Results MSCs can be effectively induced to form neurospheres by bFGF and EGF.The neurospheres can passage and differentiated into neural cells.Immunofluorescence and Western blotting showed these neurospheres expressed nestin protein. Cells differentiated from neurospheres expressed marker proteins of neurons, glial cells and oligodendrocytes. Conclusion The results indicate that MSCs can be effectively induced to form neurospheres by bFGF and EGF. The neurospheres may amplify and differentiate into neurons, glial cells and oligodendrocytes./P>

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