›› 2011, Vol. 42 ›› Issue (3): 340-344.doi: 10.3969/j.issn.0529-1356.2011.03.010

• 论著 • 上一篇    下一篇

靛红衍生物IF203通过线粒体途径诱导人肝癌HepG2细胞凋亡

尚应辉;孟书聪;王明群;董晓敏;张页*;肖军军*   

  1. 北京大学基础医学院细胞生物学系,北京市100191
  • 收稿日期:2010-11-05 修回日期:2010-11-24 出版日期:2011-06-06
  • 通讯作者: 张页;肖军军

IF203 induced apoptosis in human liver HepG2 cells through mitochondrial pathway

  1. Department of Cell Biology, Basic Medical College, Peking University, Beijing 100191, China
  • Received:2010-11-05 Revised:2010-11-24 Online:2011-06-06
  • Contact: ZHANG Ye;XIAO Jun-jun

关键词: 吲哚满酮类化合物, 线粒体膜电位, 免疫印迹法, HepG2细胞

Abstract: Objective To study the involvement of mitochondrial pathway in IF203 induced apoptosis in human liver HepG2 cells. Methods HepG2 cells were cultured in DMEM containing 10% fetal bovine serum at 37 ℃ in a humidified atmosphere with 5% COSUB>2/SUB>Inverted phase contrast microscope was used to detect the morphologic changes; Acid phosphatase assay(APA), flow cytometry(FCM)and Western blotting were used to detect the changes of cell viability, apoptosis ratio, mitochondrial transmembrane potential, the expressions of apoptosis-related protein Caspase-9, Caspase-3 and cytochrome C. Results Cell growth was inhibited after HepG2 cells were co-cultured with different concentration of IF203 for 24 hours and 48 hours. HepG2 cells showed round and shrank after they were co-cultured with IF203 of 10 mg/L. Apoptosis ratios caused by IF203 were increased. Mean fluorescence intensity (MFI) of mitochondrial transmembrane potentials in HepG2 cells decreased. The expressions of apoptosis-related protein Caspase-9 and Caspase-3 decreased, but the cytochrome C expression increased. Conclusion IF203 could significantly restrain cell growth of liver cancer cell line HepG2- IF203 can activate mitochondrial signaling pathway which would induce apoptosis, b

Key words: IF203, Mitochondrial transmembrane potential, Western blotting, HepG2 cell 

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